Method for producing rabies virus antigens for animals at a large scale
A rabies virus, large-scale technology, applied in the field of veterinary biological products, can solve the problems of no special and effective treatment for rabies, large differences between batches, unstable quality, etc., to save costs and labor, easy control, and reduce production. cost effect
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[0032] 1. Viruses and cell lines
[0033] The virus strain used to prepare the rabies virus antigen is the LEP-Flury strain, which has no pathogenicity after pathogenicity test. The hamster kidney cell (BHK21), a cell line with good sensitivity to the virus strain, is used as the cell line for vaccine production, so as to infect and multiply the rabies virus in large quantities.
[0034] 2. Preparation method
[0035] (1) Inoculate the cell line hamster kidney cells (BHK21) for the preparation of antigen into DMEM liquid medium (including 5% bovine serum, 0.01mol / L NaHCO 3 , 0.1mg / ml kanamycin sulfate (kanamycinsulfate), 100,000IU penicillin G sodium salt (Penicillin G Sodium), pH value is 7.2) and in the carrier tank of BioNoc II polyester fiber; Add 5.5 g of microcarriers, and the initial inoculum size of cells is 2.7 × 10 7 cells / g microcarriers.
[0036] (2) At 37°C, 5% CO 2 Under the culture environment, the cells are attached for 4 hours, the above-mentioned cells f...
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