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Method for preparation of oligopeptides by solid state fermentation cake

A solid-state fermentation and oligopeptide technology is applied in the field of bioengineering to achieve the effects of uniform and convenient turning of materials, simple production process and cost reduction.

Active Publication Date: 2015-02-25
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the same microorganism has slight differences in its structure during the growth and reproduction process, it is difficult to effectively stimulate all microbial cells with fixed-frequency ultrasonic waves

Method used

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  • Method for preparation of oligopeptides by solid state fermentation cake
  • Method for preparation of oligopeptides by solid state fermentation cake

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Take 100 kg of soybean meal and 100 kg of rapeseed meal, grind them through a 60-mesh sieve, and mix them for later use. Prepare seed liquid culture medium, its composition is peptone 1.0% (w / v), sodium chloride 0.5% (w / v), beef extract 0.3% (w / v), mix well, sterilize from the attached figure 1 Add the sterilized swept-frequency ultrasonic auxiliary culture tank 1 into the feeding port 5 and inoculate the activated Bacillus subtilis from the feeding port 5 Bacillus subtilis to the culture tank, control the culture temperature at 35°C, and adjust the attached figure 1In the ultrasonic working mode of the ultrasonic generator 3, the ultrasonic vibrator 2 is controlled by the wire 6, and the frequency-sweeping ultrasonic is used to assist cultivation for 24 hours, the ultrasonic frequency is 28kHz, and the sweeping frequency range is ±2kHz. Feed port 7 emits seed culture solution for subsequent use. Following the same procedure and medium recipe, use another swept-fre...

Embodiment 2

[0044] Take 100 kg of peanut meal and 100 kg of sunflower seed meal, grind them through an 80-mesh sieve, and mix them for later use.

[0045] Prepare seed liquid culture medium, its composition is the same as embodiment 1, after mixing, sterilizing, pour into sterilized frequency-sweeping ultrasonic auxiliary culture tank, inoculate activated Bacillus subtilis Bacillus subtilis Into the culture tank, 35 ℃ frequency sweep ultrasonic assisted cultivation for 36 hours, the ultrasonic frequency is 40kHz, and the sweep frequency range is ±0.5kHz. According to the same procedure and medium formula, use another frequency-sweeping ultrasonic-assisted culture tank for Aspergillus niger Aspergillus niger Ultrasound-assisted culture was carried out for 24 hours, the ultrasonic frequency was 20 kHz, and the scanning range was ±1.5 kHz. Inoculate and ferment after mixing at a volume ratio of 1:1. Inoculate the two seed culture solutions into the mixture of soybean meal and rapeseed me...

Embodiment 3

[0050] Take 100 kg of sesame meal and 100 kg of linseed meal, grind them through a 70-mesh sieve, and mix them for later use.

[0051] Prepare seed liquid culture medium, its composition is the same as that of Example 1, mix evenly, pour into sterilized frequency-sweeping ultrasonic-assisted culture tank after sterilization, inoculate activated Bacillus subtilis into the culture tank, 35 ℃ frequency-sweeping ultrasonic-assisted culture for 24 hours , Ultrasonic frequency 68kHz, sweep frequency range ± 2kHz. According to the same procedure and medium formula, another ultrasonic-assisted culture tank was used to conduct ultrasonic-assisted culture of Saccharomyces cerevisiae for 36 hours, with an ultrasonic frequency of 40 kHz and a frequency sweep range of ±1 kHz. .

[0052] Inoculate the two seed culture solutions into the mixture of soybean meal and rapeseed meal respectively according to the mass ratio of culture solution and cake 1:40, adjust the moisture content of the ca...

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Abstract

The invention relates to a method for preparation of oligopeptides by solid state fermentation cake, and relates to the field of biological engineering. One or more cakes as a raw material is crushed for pretreatment, a sweep frequency ultrasonic assisted cultured microbial fermentation liquid seed is inoculated, the cake moisture content is adjusted to 50%-60% (w / w), field assisted fermentation is performed for 60-80 hours at 30 to 35 DEG C in a multi-layer conveyer belt type fermentation bed, after the fermentation is completed, water is added for extracting the oligopeptides, and a cake oligopeptide product is obtained by centrifugal slag removal, decolorization, drying and other steps of extracting solution. The method can significantly reduce the cost of preparation of the oligopeptides by the solid state fermentation cake, and improve the production efficiency. The oligopeptides prepared by the method can be used for human health and nutrition products, animal daily diets and the like.

Description

[0001] Technical field: [0002] The present invention relates to the field of bioengineering, in particular to polypeptide production and bio-fermentation technology, in particular to the use of oil cake as the main raw material, the use of frequency-sweeping ultrasonic-assisted seed production, and the preparation of oligomers by magnetic field-assisted solid-state fermentation on a multi-layer conveyor belt type fermentation bed. peptide method. Background technique: [0003] my country's oil cake resources are extremely rich, and most of the cakes are generally directly added to the feed as feed. The processing methods are relatively extensive, resulting in low production efficiency, and some cake proteins are severely denatured by heat during oil processing, causing animals The absorption and utilization rate of the food is not high; some cakes contain anti-nutritional factors such as plant lectin, trypsin inhibitor (soybean meal) or glucosinolates, erucic acid (rapes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P21/06C12R1/125C12R1/84C12R1/865C12R1/685
Inventor 何荣海王治平邢欢马海乐蒋边朱培培丁文慧
Owner JIANGSU UNIV
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