Method for rapidly preparing man-made pit mud through functional microbial group of high-yield butyric acid and caproic acid
A functional microorganism and butyric acid production technology, applied in the field of wine making, can solve the problems of prolonging the preparation time and quality of artificial pit mud, limiting the inoculum amount of pit mud, etc., and achieve the effect of increasing the initial content, shortening the time, and reducing the inoculum amount
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[0043] Embodiment one, adopt the method of the present invention to prepare artificial pit mud
[0044]1. Select the bacterial strain Clostridium butyricum (Clostridium butyricum, preservation number: ATCC19398) and Clostridium tyrobutyricum (Clostridium tyrobutyricum, preservation number: ATCC25755) with the ability to produce butyric acid and the bacterial strain Clostridium kluyveri (Clostridium kluyveri) with the ability to produce caproic acid , preservation number: ATCC8527) were inoculated and fermented in semi-synthetic yellow water medium in a 50L fermenter, and the formula of the used semi-synthetic yellow water medium was sodium acetate 5gg / L, ammonium sulfate 0.5gg / L, hydrogen phosphate Potassium 0.4gg / L, magnesium sulfate 0.2gg / L, yeast extract 1gg / L, cysteine hydrochloride 0.5gg / L, add 1:1 sterile yellow water (yellow water diluted with equal volume of water) to 1000mL , adjust the pH value to 6.0, and sterilize at 115°C for 20min. The liquid volume of the fer...
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