A method for rapid preparation of artificial pit mud by using functional microbial flora with high production of butyric acid and caproic acid
A technology of functional microorganisms and pit mud, applied in the field of winemaking, can solve the problems of prolonging the preparation time and quality of artificial pit mud, limiting the inoculation amount of pit mud, etc., and achieves the effects of increasing initial content, shortening time and reducing inoculation amount.
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[0043] Embodiment one, adopt the method of the present invention to prepare artificial pit mud
[0044]1. Select Clostridium butyricum (Clostridium butyricum, storage number: ATCC19398) and Clostridium tyrobutyricum (Clostridium tyrobutyricum, storage number: ATCC 25755) with the ability to produce butyric acid and the bacterial strain Clostridium butyricum with the ability to produce caproic acid Bacteria (Clostridium kluyveri, preservation number: ATCC 8527) were inoculated and fermented in a semi-synthetic yellow water medium in a 50L fermenter, and the formula of the semi-synthetic yellow water medium used was sodium acetate 5g g / L, ammonium sulfate 0.5g g / L, dipotassium hydrogen phosphate 0.4g g / L, magnesium sulfate 0.2g g / L, yeast extract 1g g / L, cysteine hydrochloride 0.5g g / L, add 1:1 sterile yellow water (equal volume of water Diluted yellow water) to 1000mL, adjust the pH to 6.0, and sterilize at 115°C for 20min. The liquid volume of the fermentation tank is 20L,...
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