Probiotic bacterium preparation for chicken and preparing method of preparation
A technology of probiotics and bacterial preparations, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems that the use of probiotics needs to be further studied, and achieve the effect of improving resistance and reducing usage
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[0019] Carry out the preparation of bacterial preparation according to the following steps:
[0020] 1) Activate the slant surfaces of each strain stored at 4°C, and culture them in shake flasks (500ml-2L). Bacillus subtilis was cultured in LB medium, and the specific formula was as follows: 10 grams of tryptone, 10 grams of yeast extract, 5 grams of sodium chloride, 1000 mL of distilled water, pH 7.3, and sterilized at 121°C for 20 minutes. Incubate at 35°C for 15 hours. Brewer's yeast was cultured in YPD medium, the specific formula was as follows: 10 grams of tryptone, 20 grams of yeast extract, 20 grams of glucose, 1000 mL of distilled water, natural pH, sterilized at 121 ° C for 20 minutes. Incubate at 30°C for 24 hours. 10.0 grams of MRS casein peptone for Lactobacillus casei, 10.0 grams of beef extract, 5.0 grams of yeast extract, 5.0 grams of glucose, 5.0 grams of sodium acetate, 2.0 grams of diamine citrate, 1.0 grams of Tween-80, 2.0 dipotassium hydrogen phosphate ...
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