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Probiotic bacterium preparation for chicken and preparing method of preparation

A technology of probiotics and bacterial preparations, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems that the use of probiotics needs to be further studied, and achieve the effect of improving resistance and reducing usage

Inactive Publication Date: 2015-10-21
SRICK TIANJIN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited level of scientific research and production, its bacterial activity and the combined use of different probiotics still need to be further studied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0019] Carry out the preparation of bacterial preparation according to the following steps:

[0020] 1) Activate the slant surfaces of each strain stored at 4°C, and culture them in shake flasks (500ml-2L). Bacillus subtilis was cultured in LB medium, and the specific formula was as follows: 10 grams of tryptone, 10 grams of yeast extract, 5 grams of sodium chloride, 1000 mL of distilled water, pH 7.3, and sterilized at 121°C for 20 minutes. Incubate at 35°C for 15 hours. Brewer's yeast was cultured in YPD medium, the specific formula was as follows: 10 grams of tryptone, 20 grams of yeast extract, 20 grams of glucose, 1000 mL of distilled water, natural pH, sterilized at 121 ° C for 20 minutes. Incubate at 30°C for 24 hours. 10.0 grams of MRS casein peptone for Lactobacillus casei, 10.0 grams of beef extract, 5.0 grams of yeast extract, 5.0 grams of glucose, 5.0 grams of sodium acetate, 2.0 grams of diamine citrate, 1.0 grams of Tween-80, 2.0 dipotassium hydrogen phosphate ...

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PUM

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Abstract

The invention discloses a probiotic bacterium preparation for chicken and a preparing method of the preparation. The probiotic bacterium preparation is a mixed flora mainly composed of bacillus subtilis, beer yeast and lactobacillus casei; the probiotic bacterium preparation comprises, by weight, 50%-70% of the bacillus subtilis, 10%-30% of the beer yeast and 20%-40% of the lactobacillus casei; and the preparing method of the micro-ecology probiotic bacterium preparation includes the following steps that culture excitation, first-stage liquid enlarging cultivation, second-stage liquid enlarging cultivation and fixation to rice chaff are carried out; drying and smashing are carried out; and the bacillus subtilis, the beer yeast and the lactobacillus casei are mixed according to the proportion that the bacillus subtilis ranges from 50% to 70%, the beer yeast ranges from 10% to 30%, and the lactobacillus casei ranges from 20% to 40%. The micro-ecology dominant flora is formed in the intestines and stomach of the chicken through the probiotic bacterium preparation, nutrition conversion can be effectively improved, growth of the chicken is promoted, and the feed conversion ratio is reduced.

Description

technical field [0001] The invention relates to a poultry probiotic preparation and a preparation method thereof. Background technique [0002] Probiotics refer to microorganisms that have medical and health effects on human and animal bodies by improving the balance of microorganisms and enzymes in the body, stimulating specific or non-specific immune mechanisms. [0003] Animal probiotic preparations, as a new type of biotechnology product, have the advantages of non-toxicity, no side effects, promoting animal growth, improving feed conversion rate, and replacing antibiotics for feeding, and have attracted widespread attention. Types of animal probiotics and their products From the perspective of bacteria species, microorganisms commonly used in compound feeds include some species of Lactobacillus, Bacillus, Saccharomgces and Aspergillus . All countries are screening their own bacterial strains. The U.S. Food and Drug Administration and the American Feed Management Assoc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A23K1/18A23K1/16C12R1/125C12R1/865C12R1/245
Inventor 曲荟芬李琳
Owner SRICK TIANJIN BIO TECH
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