Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Disease-resistant growth-promoting mixed microbial flora

A technology of mixing microorganisms and flora, applied in the field of microorganisms, can solve the problems of polluting the environment, affecting product quality and safety, and high toxicity of chemical agents

Active Publication Date: 2021-07-27
成都云图控股股份有限公司
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, cucumber root-knot nematodes are becoming more and more harmful in the facilities of continuous cropping for many years. Chemical control is mainly used for its prevention and control. Chemical agents are highly toxic and ineffective, and pollute the environment and affect the quality and safety of products.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Disease-resistant growth-promoting mixed microbial flora
  • Disease-resistant growth-promoting mixed microbial flora
  • Disease-resistant growth-promoting mixed microbial flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A disease-resistant and growth-promoting mixed microbial flora, including Streptomyces jute, Bacillus paralicheniformis and cellulolytic rhizobia; the microbial flora is prepared by the following method: reconciling Streptomyces jute, Bacillus paralicheniformis The cellulosic rhizobia species were inoculated on LB solid medium for activation, respectively inoculated in LB liquid medium according to the inoculum amount of 2% to expand the culture, and respectively obtained 1.5×10 10 CFU / mL fermentation broth, mixed in proportion to obtain the mixed bacterial solution of the present invention.

[0024] Further, the composition of the LB medium is: tryptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000mL, pH 7.2-7.4, sterilized at 121°C for 19min, solid medium plus 15g agar powder.

[0025] Further, the conditions for the activation of the strains on the LB solid medium are: constant temperature cultivation at 25° C. for 10 h.

[0026] Further, the conditions for ...

Embodiment 2

[0035] A disease-resistant and growth-promoting mixed microbial flora, including Streptomyces jute, Bacillus paralicheniformis and cellulolytic rhizobia; the microbial flora is prepared by the following method: reconciling Streptomyces jute, Bacillus paralicheniformis The cellulosic rhizobia species were inoculated on LB solid medium for activation, respectively inoculated in LB liquid medium according to the inoculum amount of 2% to expand the culture, and respectively obtained 1.5×10 10 CFU / mL fermentation broth, mixed in proportion to obtain the mixed bacterial solution of the present invention.

[0036] Further, the composition of the LB medium is: tryptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000mL, pH 7.2-7.4, sterilized at 121°C for 19min, solid medium plus 15g agar powder.

[0037]Further, the conditions for the activation of the strains on the LB solid medium are: constant temperature cultivation at 28° C. for 15 hours.

[0038] Further, the conditions f...

Embodiment 3

[0047] A disease-resistant and growth-promoting mixed microbial flora, including Streptomyces jute, Bacillus paralicheniformis and cellulolytic rhizobia; the microbial flora is prepared by the following method: reconciling Streptomyces jute, Bacillus paralicheniformis The cellulosic rhizobia species were inoculated on LB solid medium for activation, respectively inoculated in LB liquid medium according to the inoculum amount of 2% to expand the culture, and respectively obtained 1.5×10 10 CFU / mL fermentation broth, mixed in proportion to obtain the mixed bacterial solution of the present invention.

[0048] Further, the composition of the LB medium is: tryptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000mL, pH 7.2-7.4, sterilized at 121°C for 19min, solid medium plus 15g agar powder.

[0049] Further, the conditions for the activation of the strains on the LB solid medium are: constant temperature cultivation at 28° C. for 18 hours.

[0050] Further, the conditions ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a disease-resistant growth-promoting mixed microbial flora, and belongs to the technical field of microorganisms. The flora provided by the invention comprises streptomyces corchorusii, bacillus paralicheniformis and cellulose nodule bacteria; wherein the microbial flora is prepared by the following method: respectively inoculating streptomyces corchorusii, bacillus paralicheniformis and cellulose rhizobium strains on an LB solid culture medium for activation, respectively inoculating the strains into an LB liquid culture medium according to the inoculum size of 2% for amplification culture to respectively obtain 1.5 * 10<10> CFU / mL fermentation broth, and mixing the fermentation broth according to the proportion. According to the invention, strains with biocontrol and growth promotion functions are screened to form a mixed flora, synergistic interaction is realized, cucumber root knot nematode diseases are effectively prevented and controlled, and the quality and yield of cucumbers are effectively improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a disease-resistant and growth-promoting mixed microbial flora. Background technique [0002] Root-knot nematode (Meloidogyne spp.) is an important class of soil-borne pathogens. There are different degrees of root-knot nematode damage to greenhouse vegetables in my country, and the annual economic losses are very large. At present, the application of chemical pesticides is still the main measure to control nematode diseases, and the resulting environmental and food safety problems have become the main obstacles to the development of organic agriculture. At present, cucumber root-knot nematodes are becoming more and more harmful in continuous cropping facilities. Chemical control is the main way to control them. Chemical agents are highly toxic and ineffective, and they pollute the environment and affect the quality and safety of products. A large number of s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/28A01N63/22A01N63/20A01P5/00A01P21/00C12R1/465C12R1/07C12R1/41
CPCC12N1/20A01N63/28A01N63/22A01N63/20
Inventor 柏万文阎应广黄亦蒋天举
Owner 成都云图控股股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com