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Mixed microorganism flora for promoting growth of plant roots

A technology of mixed flora and microorganisms, applied in the direction of plant growth regulators, plant growth regulators, microorganisms, etc., can solve problems such as increased planting costs, decreased quality of agricultural products, and non-point source pollution, and achieves stable, controllable, and tolerant formulations Capable, Adaptable Effects

Inactive Publication Date: 2019-09-24
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to achieve this goal, technicians in the planting industry often blindly use excessive fertilizers, especially chemical fertilizers, which will not only increase planting costs and waste resources, but also cause problems such as soil compaction and acidification of cultivated land and decline in the quality of agricultural products. Non-point source pollution that seriously affects the quality of surface water and groundwater

Method used

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  • Mixed microorganism flora for promoting growth of plant roots
  • Mixed microorganism flora for promoting growth of plant roots
  • Mixed microorganism flora for promoting growth of plant roots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Microbial mixed flora promotes growth of hydroponic plants

[0020] The specific implementation steps are as follows:

[0021] Bacillus megaterium CGMCC10466 (denoted as bacteria A), Pseudomonas brassicacearum CGMCC10355 (denoted as bacteria B), and Azospirillum brasilense CGMCC5806 (denoted as bacteria C) were inoculated into LB medium respectively, 30 Cultivate with shaking at ℃ until the bacterial concentration is O.D. 600 ≈2.0, mixed according to the volume ratio of 1:1:1, 1:2:1, 1:1:2, 2:1:1 respectively, and at the same time, two kinds of bacteria were selected and mixed in the volume ratio of 1:1, and then Take the single-bacteria culture solution of three kinds of bacteria. Each of the above-mentioned culture solutions was prepared according to the 10 6 The final microbial concentration of CFU / ml was added to the rhizosphere of rapeseed in the hydroponic system, and the culture was continued for 1 week. In the present invention, a blank control group is also...

Embodiment 2

[0026] Microbial mixed flora promotes soil culture plant growth

[0027] The specific implementation steps are as follows:

[0028] Bacillus megaterium CGMCC10466 (denoted as bacteria A), Pseudomonas brassicacearum CGMCC10355 (denoted as bacteria B), and Azospirillum brasilense CGMCC5806 (denoted as bacteria C) were inoculated into LB medium respectively, 30 Cultivate with shaking at ℃ until the bacterial concentration is O.D. 600 ≈2.0, mixed according to the volume ratio of 1:1:1, 1:2:1, 1:1:2, 2:1:1 respectively, and at the same time, two kinds of bacteria were selected and mixed in the volume ratio of 1:1, and then Take the single-bacteria culture solution of three kinds of bacteria. Each of the above-mentioned culture solutions was prepared according to the 10 6 The final microbial concentration of CFU / ml was added to the rhizosphere of rapeseed in the soil culture system, and the culture was continued for 1 week. Under the same conditions, an equal amount of water was...

Embodiment 3

[0034] Microbial strains secreting brassinosteroids

[0035] The specific implementation steps are as follows:

[0036] Bacillus megaterium CGMCC10466 (denoted as bacteria A), Pseudomonas brassicacearum CGMCC10355 (denoted as bacteria B), and Azospirillum brasilense CGMCC5806 (denoted as bacteria C) were inoculated into LB medium respectively, 30 Cultivate with shaking at ℃ until the bacterial concentration is O.D. 600 ≈2.0. Take 100mL of the three bacterial cultures respectively, and use high-performance liquid chromatography-tandem mass spectrometry to determine the content of brassinosteroids therein, as shown in Table 3 below.

[0037] Table 3 The results of the strain secretion of brassinosteroids

[0038]

[0039]

[0040]It can be seen from Example 3 that all three strains have the ability to secrete brassinosteroids, which can promote the growth of plant roots and increase crop yield in the actual environment. As can be seen from the experimental data of Exam...

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Abstract

The invention discloses a mixed microorganism flora for promoting growth of plant roots, and belongs to the technical field of agricultural microorganisms. The mixed flora is composed of bacillus megatherium, pseudomonas brassicacearum and azospirillum brasilense; the mixed microorganism flora is prepared by adopting a following method. The preparation method comprises the steps that the bacillus megatherium, the pseudomonas brassicacearum and the azospirillum brasilense are separately inoculated in an LB culture medium, vibration culture is conducted at 30 DEG C until the bacterial concentration O.D600 is about 2.0, then the bacillus megatherium, the pseudomonas brassicacearum and the azospirillum brasilense are mixed according to the volume ratio of 1:1:1, and the mixed microorganism flora is obtained. The mixed microorganism flora is superior to a single bacterial strain, all the bacterial strains have a synergistic effect, a significant effect of promoting growth of plant root systems is achieved, and the mixed microorganism flora quite highly adapts to actual soil rhizosphere complex environments.

Description

technical field [0001] The invention relates to a mixed flora composed of multiple microbial strains, in particular to a method for the flora to promote the growth of plant roots, and belongs to the technical field of agricultural microorganisms. Background technique [0002] Promoting yield increase is the primary task of modern high-efficiency agriculture. In order to achieve this goal, technicians in the planting industry often blindly use excessive fertilizers, especially chemical fertilizers, which will not only increase planting costs and waste resources, but also cause problems such as soil compaction and acidification of cultivated land and decline in the quality of agricultural products. Non-point source pollution that seriously affects the quality of surface water and groundwater. Therefore, controlling the amount of chemical fertilizers is a practical need to promote cost savings, energy conservation and emission reduction, and is of great significance to ensure ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/11C12R1/38C12R1/01
CPCA01N63/00C12N1/20
Inventor 李强许菡亓文钰韩静
Owner LINYI UNIVERSITY
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