Skin barrier repairing complex, facial cream and preparation method therefor
A skin barrier and complex technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve problems such as skin barrier damage, maintain steady state and basic health, resist external pressure, and improve skin resistance. The effect of stress
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preparation example 1
[0072] This preparation example prepares a sea fennel callus culture filtrate, and the preparation method of the sea fennel callus culture filtrate comprises the following steps:
[0073] (1) Callus induction culture: sea fennel explants were inoculated into agar medium for callus induction culture, the temperature of the induction culture was 20°C, and the time of the induction culture was 14 days, and the callus was obtained. injured tissue;
[0074] (2) Callus particle suspension culture: inoculate the callus obtained in step (1) into MS medium, and carry out shaking culture in a shaking incubator at a speed of 100 r / min, and the temperature of the shaking culture is 20°C , the time of the shaking culture is 14 days to obtain the proliferation callus;
[0075] (3) Expanded culture of callus: Inoculate the proliferated callus obtained in step (2) into a sterile cell bag for expanded culture, the temperature of the expanded culture is 25°C, and the time of the expanded cultu...
preparation example 2
[0078] This preparation example prepares tetrahydromethylpyrimidine carboxylic acid, and the preparation method of described tetrahydromethylpyrimidine carboxylic acid comprises the following steps:
[0079] (A) Cultivation of desert halophilic bacteria: the desert halophilic bacteria were inoculated in SCDLP liquid medium, and cultivated with a rotating speed of 1000r / min in a shaking incubator, and the temperature of the cultivation was 20°C. The time is 48h, and the desert halophilic bacteria cell is obtained;
[0080] (B) Osmotic pressure shock: the desert halophilic bacteria bacterium that step (A) obtains is resuspended in hyperosmotic pressure solution (the hyperosmotic pressure solution comprises: 18% sucrose, 0.1% EDTA, remaining Soak in water) for 1 hour, filter to obtain the precipitated desert halophilic bacterial cell;
[0081](C) Electrodialysis: the desert halophilic bacterial cells obtained in step (B) are treated by electrodialysis to obtain tetrahydromethyl ...
preparation example 3
[0083] This preparation example prepares a kind of hydrolyzed red algae extract, the preparation method of described hydrolyzed red algae extract comprises the following steps:
[0084] (a) Extraction: wash, crush and pass through a 100-200 mesh sieve to obtain the red algae powder; add water to the red algae powder and perform an extraction at 70°C, and the time for the first extraction is 10 hours , the mass ratio of the water and the red algae powder is 20:1, the primary extract is obtained, the primary extract is filtered and then concentrated, then the volume ratio concentration is added to an aqueous ethanol solution of 80% for secondary extraction, and the precipitate is left to stand, Obtain red algae polysaccharide crude material;
[0085] (b) Hydrolysis: after dissolving the crude red algae polysaccharide obtained in step (a) with water, add 30wt% hydrogen peroxide aqueous solution, and mix and stir at 50°C for 1 hour to obtain a hydrolyzed red algae extract;
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