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Microbial electricity generation system comprising mixed bacterial flora and microbial fuel cell

A technology of mixing flora and microorganisms, applied in the fields of biochemical fuel cells, microorganisms, microorganism-based methods, etc., can solve the problems of poor system stability and repeatability, complex system, short duration of electricity generation, etc., to enhance electron transfer efficiency. , the effect of simple system

Active Publication Date: 2017-05-31
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stability and repeatability of the system are poor, the duration of power generation is short, and dozens of mineral and vitamin solutions need to be added, which is costly and complicated

Method used

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  • Microbial electricity generation system comprising mixed bacterial flora and microbial fuel cell
  • Microbial electricity generation system comprising mixed bacterial flora and microbial fuel cell
  • Microbial electricity generation system comprising mixed bacterial flora and microbial fuel cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1 mixed flora

[0058] Construction of Bacillus subtilis with high riboflavin production: Through genetic manipulation, the prs and ywlF genes in Bacillus subtilis were overexpressed, and the Pur operon and PurR regulatory genes (glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO).

[0059] Construct Escherichia coli that produces small molecular acids using five-carbon sugars and six-carbon sugars as carbon sources: use λ-Red homologous recombination technology to knock out the pflB gene in Escherichia coli, and then introduce the ldhE gene (lactic acid-producing gene, derived from Lactobacillus, Synthesized by GENWIZE company), the method is: by EcoRI and PstI digestion, connected to the pSB1C plasmid, and then the plasmid connected with the ldhE gene is introduced into the above-mentioned Escherichia coli with the pflB gene knocked out, and the chloramphenicol resistance is screened, and the correct transformants. Take the constructed Bacillus subtilis with...

Embodiment 2

[0062] Embodiment 2 mixed flora

[0063] Construction of Bacillus subtilis with high riboflavin production: Through genetic manipulation, the prs and ywlF genes in Bacillus subtilis were overexpressed, and the Pur operon and PurR regulatory genes (glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO).

[0064] Construct Escherichia coli that produces small molecular acids using five-carbon sugars and six-carbon sugars as carbon sources: use λ-Red homologous recombination technology to knock out the pflB gene in Escherichia coli, and then introduce the ldhE gene (lactic acid-producing gene, derived from Lactobacillus, Synthesized by GENWIZE company), the method is: by EcoRI and PstI digestion, connected to the pSB1C plasmid, and then the plasmid connected with the ldhE gene is introduced into the above-mentioned Escherichia coli with the pflB gene knocked out, and the chloramphenicol resistance is screened, and the correct transformants.

[0065] Take the constructed Bacillus subt...

Embodiment 3

[0068] Embodiment 3 mixed flora

[0069] Construction of Escherichia coli with high riboflavin production 1: Introducing the ribABDEC gene cluster into Escherichia coli;

[0070] Construction of Escherichia coli 2 that uses five-carbon sugars and six-carbon sugars as carbon sources to produce small molecular acids: the pflB gene was knocked out in Escherichia coli using λ-Red homologous recombination technology, and then the ldhE gene (lactic acid-producing gene, derived from Lactobacillus , synthesized by GENWIZE company), the method is: digested with EcoRI and PstI, connected to the pSB1C plasmid, and then the plasmid with the ldhE gene is introduced into the above-mentioned Escherichia coli with the pflB gene knocked out, and the resistance to chloramphenicol is screened to screen out correct transformants.

[0071] Take the constructed Escherichia coli 1 with high riboflavin production, the constructed Escherichia coli 2 that uses five-carbon sugars and six-carbon sugars ...

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Abstract

The invention relates to the field of microbial electricity generation and especially relates to a microbial electricity generation system comprising a mixed bacterial flora and a microbial fuel cell. In the invention, an engineered mixed bacterial flora is constructed, wherein each of the bacteria has definite effects. A first fermentation bacteria provides a carrier, riboflavin, for electron transfer for the system; a second fermentation bacteria converts the most common and low-cost glucose into small-molecular acids for the electricity generating bacteria, thereby enlarging carbon source spectrum of the electricity generating bacteria. In the invention, escherichia coli or bacillus subtilis, which are model strains of prokaryotes Gram negative bacteria and Gram positive bacteria, are selected, through genetic engineering modification and the like, two fermentation bacteria having different applications are constructed, so that the carbon source spectrum of the microbial fuel cell is enlarged and electron transfer efficiency of the electricity generating bacteria is increased. Meanwhile, from three important angles, including material flow, energy flow and information flow, a stable, reasonable and high-effective functional mixed bacterial symbiotic electricity generation system is constructed.

Description

technical field [0001] The invention relates to the field of microbial electricity generation, in particular to a microbial electricity generation system and a microbial fuel cell containing the mixed flora. Background technique [0002] Microbial Fuel Cell (MFC) is a device that uses microorganisms to directly convert chemical energy in organic matter into electrical energy. Its basic working principle is: in the anaerobic environment of the anode chamber, the organic matter decomposes under the action of microorganisms and releases electrons and protons. It is effectively transmitted between the anode and the electrode, and is transmitted to the cathode through an external circuit to form a current, while the proton is transmitted to the cathode through the proton exchange membrane, and the oxidant gets electrons at the cathode and is reduced and combined with the proton to form water. [0003] Compared with other existing technologies that use organic matter to generate ...

Claims

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Application Information

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IPC IPC(8): C12N1/20H01M8/16C12R1/19C12R1/125C12R1/01
CPCY02E60/50Y02P70/50
Inventor 元英进刘悦宋浩凌威林童
Owner TIANJIN UNIV
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