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Method for increasing lactic acid fermentation yield of kitchen waste employing hydro-thermal treatment

A technology for food waste and hydrothermal treatment, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of high cost of lactic acid fermentation process, difficulty in meeting modern production, low yield of lactic acid fermentation, etc., to achieve Good fermentation effect, low production cost and simple process

Active Publication Date: 2014-04-02
SOUTH CHINA AGRI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, some of the disclosed strains are difficult to obtain and have low bioavailability, resulting in high cost of lactic acid fermentation process; meanwhile, the yield of conventional strains for lactic acid fermentation is not high, which is difficult to meet the needs of modern production
At present, there is no report on the synergistic application of Lactobacillus bulgaricus, Streptococcus thermophilus, and Bacillus coagulans in lactic acid fermentation in the prior art

Method used

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  • Method for increasing lactic acid fermentation yield of kitchen waste employing hydro-thermal treatment

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Experimental program
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Embodiment 1

[0034] 1. Preliminarily sort the collected food waste, and remove the hard-to-ferment debris to obtain 4 kg of food waste, put it into a hydrothermal reactor, add 5 times the volume of water, and conduct a hydrothermal treatment at 150°C for 50 minutes; The motor was turned off after stirring for 20 minutes.

[0035] 2. After the hydrothermal treatment, the food waste is sprayed into the three-phase separator, and the grease and water-containing solid food waste are separated after standing.

[0036] 3. Send the separated water-containing solid kitchen waste to the fermenter, add the stored three-phase separated kitchen waste water to make the solid-liquid volume ratio 1:12; add 8% calcium carbonate by weight of the solid kitchen waste; then Add the compound enzyme of 2% of solid food waste weight, the mass ratio of various enzymes in the compound enzyme is cellulase: lipase: amylase: protease: lactate dehydrogenase=0.8:2:1:1:0.8; The composition of the enzyme preparation is:...

Embodiment 2

[0038]The main steps of this embodiment are the same as in Example 1, except that in step 1, when the food waste contains more water, after removing the impurities that are not easy to ferment, it is directly hydrothermally treated at 190°C for 15 minutes, and the stirring motor is not turned on at the same time. After the hydrothermal treatment, the pressure is released. When the pressure of the hydrothermal reactor is released to 1.3atm, the kitchen waste is sprayed into the three-phase separator, and after standing still, the oil and the water exceeding the range of solid-liquid ratio required for lactic acid fermentation are separated. Make the solid-liquid volume ratio reach 1:14. Remaining solid and water are sent to fermenter, add the compound enzyme of 1% calcium carbonate of solid weight and 10% of solid weight; Wherein, the composition of compound enzyme is cellulase (200000u / g), lipase (200000u / g), amylase (200000u / g), protease (200000u / g), lactate dehydrogenase (1...

Embodiment 3

[0040] The main steps of this embodiment are the same as in Example 1, except that in step 3, 20% of the weight of solid food waste is added to the sorted food waste without hydrothermal treatment before the lactic acid fermentation. Others are the same as in Example 1. After the fermentation is finished, centrifuge and take the supernatant to purify lactic acid by conventional precipitation method. The fermented liquid was analyzed by liquid chromatography, and the concentration of lactic acid obtained reached 22g / L; after conversion, 18.3g of lactic acid was produced per 100g of kitchen waste.

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Abstract

The invention discloses a method for increasing lactic acid fermentation yield of kitchen waste employing hydro-thermal treatment. The kitchen waste is taken as a raw material for producing a high-value-added product, so that recycling of biomass is achieved when the waste is fully utilized. By combining with a hydro-thermal pretreatment technology, the lactic acid fermentation yield of the kitchen waste is increased by common action of microorganisms and complex enzyme. Simple and available microbe bacteria are selected to prepare mixed flora, and meanwhile, a common enzyme is selected to prepare the complex enzyme, so that efficient fermentation to produce lactic acid is achieved. The method is simple and convenient in fermentation technology, good in fermentation effect and especially applicable to industrial application.

Description

technical field [0001] The invention belongs to the field of kitchen waste treatment, and in particular relates to a method for improving the yield of lactic acid fermentation of kitchen waste through hydrothermal treatment. Background technique [0002] Food waste is suitable for microbial lactic acid fermentation because it is rich in organic matter. Lactic acid is purified by dehydration to produce polylactic acid, a thermoplastic aliphatic polyester. The obtained polylactic acid has good mechanical and processing properties, and the polylactic acid product can be rapidly degraded in various ways after being discarded. Therefore, polylactic acid is considered to be a green plastic with good performance. [0003] At present, many strains that can be used for lactic acid fermentation of kitchen waste have been disclosed. For example, Chinese patent (CN102424831 A) discloses the use of Bacillus licheniformis, Bacillus megaterium, Bacillus natto, Bacillus subtilis and Lacto...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/56C12R1/46C12R1/225C12R1/07
Inventor 王春铭李咏方惠青李颖邓博黄应燊
Owner SOUTH CHINA AGRI UNIV
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