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Microbial mixed flora for promoting growth of plant stems

A technology of mixing flora and microorganisms, applied in the directions of plant growth regulators, plant growth regulators, microorganisms, etc., can solve the problems of non-point source pollution, quality decline of agricultural products, soil compaction and acidification of cultivated land, etc., and achieve the effect of strong adaptability

Active Publication Date: 2019-09-17
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to achieve this goal, technicians in the planting industry often blindly use excessive fertilizers, especially chemical fertilizers, which will not only increase planting costs and waste resources, but also cause problems such as soil compaction and acidification of cultivated land and decline in the quality of agricultural products. Non-point source pollution that seriously affects the quality of surface water and groundwater

Method used

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  • Microbial mixed flora for promoting growth of plant stems
  • Microbial mixed flora for promoting growth of plant stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Microbial mixed flora promotes stem growth in hydroponic plants

[0021] The specific implementation steps are as follows:

[0022] Azotobacter beijerinckii CGMCC9044 (denoted as bacteria A), Rhizobium anhuiense CGMCC15545 (denoted as bacteria B), Paenibacillus polymyxaCGMCC15984 (denoted as bacteria C), were inoculated into LB medium respectively, 30 Cultivate with shaking at ℃ until the bacterial concentration is O.D. 600 ≈2.0, mixed according to the volume ratio of 1:1:1, 1:2:1, 1:1:2, 2:1:1 respectively, and at the same time, two kinds of bacteria were selected and mixed in the volume ratio of 1:1, and then Take the single-bacteria culture solution of three kinds of bacteria. Each of the above-mentioned culture solutions was prepared according to the 10 6 The final microbial concentration of CFU / ml was added to the rhizosphere of rapeseed in the hydroponic system, and the culture was continued for 1 week. At the same time, the present invention sets a group of b...

Embodiment 2

[0027] Microbial mixed flora promotes stem growth of soil cultured plants

[0028] The specific implementation steps are as follows:

[0029] Azotobacter beijerinckii CGMCC9044 (denoted as bacteria A), Rhizobium anhuiense CGMCC15545 (denoted as bacteria B), Paenibacillus polymyxaCGMCC15984 (denoted as bacteria C), were inoculated into LB medium respectively, 30 Cultivate with shaking at ℃ until the bacterial concentration is O.D. 600 ≈2.0, mixed according to the volume ratio of 1:1:1, 1:2:1, 1:1:2, 2:1:1 respectively, and at the same time, two kinds of bacteria were selected and mixed in the volume ratio of 1:1, and then Take the single-bacteria culture solution of three kinds of bacteria. Each of the above-mentioned culture solutions was prepared according to the 10 6 The final microbial concentration of CFU / ml was added to the rhizosphere of rapeseed in the soil culture system, and the culture was continued for 1 week. Under the same conditions, an equal amount of water ...

Embodiment 3

[0035] Determination of the ability of microbial strains to secrete gibberellin

[0036] The specific implementation steps are as follows:

[0037] Azotobacter beijerinckii CGMCC9044 (denoted as bacteria A), Rhizobium anhuiense CGMCC15545 (denoted as bacteria B), Paenibacillus polymyxaCGMCC15984 (denoted as bacteria C), were inoculated into LB medium respectively, 30 Cultivate with shaking at ℃ until the bacterial concentration is O.D. 600 ≈2.0. Take 100 mL of the three bacterial cultures respectively, and use high performance liquid chromatography-tandem mass spectrometry to determine the content of gibberellin in it, as shown in Table 3 below.

[0038] Table 3 The results of the strain secretion gibberellin experiment

[0039] Gibberellin secretion capacity (g / L) Bacteria A 10.6 Bacteria B 9.8 Bacteria C 11.2

[0040] It can be seen from Example 3 that all three strains have the ability to secrete gibberellin, which can promote the growt...

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Abstract

The invention discloses a microbial mixed flora for promoting growth of plant stems and belongs to the technical field of agricultural microorganisms. The microbial mixed flora is composed of azotobacter beijerimckii, Anhui rhizobium and paenibacillus polymyxa. The microbial mixed flora is prepared by adopting a following method. The method comprises the steps that the azotobacter beijerimckii, Anhui rhizobium and paenibacillus polymyxa are inoculated to an LB culture medium separately, shaking culture at 30 DEG C is conducted until the concentration of the flora O.D600 is about 2.0, and through mixing according to the volume ratio of 1:1:1, the microbial mixed flora is obtained. The microbial mixed flora has an obvious effect of promoting growth of the plant stems and increasing the yield of crops and has wide application prospects in agricultural production.

Description

technical field [0001] The invention relates to a microbial mixed flora for promoting the growth of plant stems, belonging to the technical field of agricultural microorganisms. Background technique [0002] Promoting yield increase is the primary task of modern high-efficiency agriculture. In order to achieve this goal, technicians in the planting industry often blindly use excessive fertilizers, especially chemical fertilizers, which will not only increase planting costs and waste resources, but also cause problems such as soil compaction and acidification of cultivated land and decline in the quality of agricultural products. Non-point source pollution that seriously affects the quality of surface water and groundwater. Therefore, controlling the amount of chemical fertilizers is a practical need to promote cost savings, energy conservation and emission reduction, and is of great significance to ensure national food security, agricultural product quality and safety, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/065C12R1/01C12R1/41
CPCA01N63/00C12N1/20Y02A40/10
Inventor 李强许菡亓文钰姜莎莎
Owner LINYI UNIVERSITY
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