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Method for producing feed from detoxified corn residue after producing alcohol by mixed bacterial micro-ecological formulation

A technology of micro-ecological preparations and mixed flora, applied in animal feed, animal feed, application, etc., can solve the problems that the adsorbent cannot be digested and absorbed by animals, is not suitable for detoxification of a large number of moldy feeds, and destroys nutrients. Enhance animal intestinal resistance, achieve high-value utilization, and promote digestion

Inactive Publication Date: 2009-04-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The physical detoxification method mainly uses adsorbents to remove mycotoxins. The amount of detoxification is limited, and most of the added adsorbents cannot be digested and absorbed by animals, which has the adverse effect of adsorbing nutrients in the feed.
The removal of mycotoxins through the action of chemical reagents is suitable for the treatment of large grains of moldy feed such as corn, but it is time-consuming and labor-intensive. Uncertainty about health hazards and environmental pollution

Method used

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  • Method for producing feed from detoxified corn residue after producing alcohol by mixed bacterial micro-ecological formulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. The preserved Candida utilis CL Y01 (purchased from the Culture Collection Center of Guangdong Institute of Microbiology, GIM2.9), Bacillus subtilis H008 (purchased from the Culture Collection Center of Guangdong Institute of Microbiology) , GIM1.135) and Lactobacillus casei subsp.rhamnosus 6013 (purchased from China Industrial Microorganism Culture Collection Center, 6013) were respectively used wort solid agar medium (purchased from Guangzhou Huankai Microbiology Co., Ltd.), LB Solid medium (tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, agar 20g / L, pH 7.0) and Lactobacillus solid medium (glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, sodium acetate 2g / L, ammonium citrate 2g / L, Tween 801mL / L, MgSO 4 ·7H 2 O 0.2g / L, K 2 HPO 4 2g / L, MnSO 4 ·H 2 O 0.05g / L, pH 6.5) were activated and cultured for 1 day.

[0027] 2. Pick a single colony and inoculate it in 10mL seed medium in a test tube (seed medium has the same composition as the activati...

Embodiment 2

[0036] Detect the efficiency of the mixed flora probiotics in degrading ZEA in liquid samples. Take the standard product ZEA and prepare 10 mL of a solution with a content of 5.23 μg / mL, and add 0.5 g of the mixed flora microecological preparation made in Step 7 of Example 1 to make the yeast concentration in the reaction system about 2.0×10 7 CFU / mL, while taking the ZEA solution without probiotics as a control. Reaction conditions: 30°C, 200r / min, shake for 5h, take samples at 0.5h, 1h, 1.5h, 3h, 5h, centrifuge at 5000r / min for 20min, take the supernatant and use ELISA to detect its ZEA content. The ZEA content of the experimental group and the control group measured by the time is plotted, as figure 1 As shown, it can be clearly seen that most of the ZEA in the system is efficiently degraded when the mixed flora probiotics are added to react for 0-1 hour, and the degradation rate of ZEA slows down when reacted for 1-5 hours. After 1 hour of reaction, the ZEA content in th...

Embodiment 3

[0038] Take 1 kg of corn alcohol residue sample and pulverize it, soak the sample in 10 mL of sterile water for 2 hours, centrifuge to get the supernatant, detect the ZEA content with ELISA, calculate the ZEA content of the compound feed sample to be about 107.15 μg / kg, mix the pulverized feed sample with 10 g Mix the mixed flora probiotics prepared in step 7 of Example 1 and mix evenly, spray sterile water to keep the humidity of the mixture above 40%, stir with a mixer at a constant speed of 30r / min, react at 30°C for 10h, and then react at 50°C After drying, a sample was taken to detect that the residual ZEA content was 6.43 μg / kg, and the detoxification rate was 94.0%.

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Abstract

The invention discloses a method for producing feedstuff by utilization of mixed flora microecological preparation for detoxication of corn alcohol residue, wherein candida utili, Bacillus subtilis and rhamnose lactobacillus are subjected to high-density fermentation, so that the viable count of the candida utili is more than 4.0X10<8>CFU / g, the viable count of the Bacillus subtilis is more than 3.8X10<8>CFU / g, and the viable count of the rhamnose lactobacillus is more than 3.0X10<8>CFU / g; and the preparation is directly mixed with the corn residue after production of alcohol or other toxic feedstuff polluted by mildew according to the proportion of between 5 and 10 grams per kilogram, and the mixture is sprayed with sterile water to maintain a humidity of more than 40 percent, reacts for 2 to 10 hours at a temperature of between 30 and 35 DEG C, is dried at a temperature of between 45 and 50 DEG C, and can degrade ZEA in the corn alcohol residue or the other toxic feedstuff, wherein the detoxication rate reaches more than 94 percent, so that the corn alcohol residue or the other toxic feedstuff becomes safe animal feedstuff.

Description

technical field [0001] The invention relates to a method for producing feed, in particular to a method for producing feed from detoxified corn alcohol residue with mixed flora probiotics. Background technique [0002] Feed and food mildew is a common problem all over the world. According to the data of the Food and Agriculture Organization of the United Nations (FAO) in 2002, about 25% of the crops and grains in the world are polluted by mycotoxins every year. The loss accounts for more than 10% of the total output, and the main mildew comes from a kind of estrogenic mycotoxin-zearalenone (ZEA) produced by Fusarium. Sampling tests from feed factories in various places found that the detection rate of ZEA in corn, protein feed and compound feed was 100%, with an average concentration of 104.99 μg / kg, which was seriously exceeded. [0003] At present, the detoxification technology of mycotoxins mainly adopts physical detoxification methods (heating, ultraviolet radiation, org...

Claims

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Application Information

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IPC IPC(8): A23K1/06A23K1/16A23K10/18A23K10/37
CPCY02P60/87
Inventor 吴晖唐语谦余以刚李晓凤刘冬梅
Owner SOUTH CHINA UNIV OF TECH
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