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Cotton fabric desizing method based on compounding of crude enzyme and amylase produced by mixed flora

A technology of mixing bacterial flora and crude enzyme liquid, applied in the field of textile, can solve the problems of low enzyme activity, few species and distribution, long cultivation period, etc., and achieves the effect of soft hand feeling and saving cultivation time.

Active Publication Date: 2016-03-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The key to realize the biodegradation of PVA is to be able to screen the microorganisms that can efficiently degrade PVA, but the types and distribution of PVA degrading bacteria are not many, the culture period is long, the enzyme activity is low, and the extraction is not easy. Important factors for actual production application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Mixed flora produces crude enzyme solution (not induced)

[0029] The mixed flora was cultivated on the broth medium to prepare seed liquid, then inoculated into the broth medium, and fermented at 30°C for 3 days; then the cells were collected, the walls were broken, and the supernatant was obtained by centrifugation. Filter with a microporous membrane (pore size 0.45um, diameter 50mm), and then dialyze with 0.1mol / L potassium phosphate buffer (pH8.0) for 24h, which is the crude enzyme solution.

[0030] The broth culture medium contains 10.0-15.0 g / L of peptone, 3.0-5.0 g / L of beef powder, 5.0-6.0 g / L of sodium chloride, and pH 7.2±0.2.

[0031] The crude enzyme solution was determined to have no PVA degrading enzyme activity.

Embodiment 2

[0032] Example 2: Adding a small molecule polyol inducer to induce the enzyme production of mixed bacteria

[0033]The mixed flora was cultured on the broth medium to prepare seed liquid, and then inoculated into the broth medium containing 4-6g / L 1,3-butanediol, and fermented at 30°C for 3 days; then the cells were collected , broken the wall, centrifuged to take the supernatant to measure the enzyme activity.

[0034] The broth medium contains peptone 10.0-15.0g / L, beef meal 3.0-5.0g / L, sodium chloride 5.0-6.0g / L, 1,3-butanediol 4-6g / L, pH 7. 2±0.2.

[0035] The results showed that the total enzyme activity of PVA-degrading enzymes could reach 1.985U / mL.

Embodiment 3

[0036] Embodiment 3: the cotton fabric desizing method based on mixed bacterial colony producing crude enzyme liquid and amylase compound

[0037] Enzymatic desizing of PVA-starch sized cotton fabrics.

[0038] (1) soak the unsized cotton fabric in PVA1799-starch solution (PVA concentration is 30-50g / L, starch concentration is 30-50g / L) for 2-10min, padding is performed, and then dried at 105°C After drying for 10 minutes, the sizing fabric was washed with water at room temperature, and the PVA-starch slurry with poor adhesion to the fabric surface was washed away. The cotton cloth sized by this method had a PVA content of 2% and a starch content of 5.6%.

[0039] (2) During desizing, steam the sizing cotton fabric for 40 min, and then immerse it in a phosphate buffered solution containing the crude enzyme solution produced by mixed bacteria and amylase with a bath ratio of 1:10 (wherein the PVA degrading enzyme is in the system). The final enzyme activity was 40U / L, the amyl...

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PUM

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Abstract

The invention discloses a cotton fabric desizing method based on compounding of crude enzyme and amylase produced by mixed flora and belongs to the technical field of textile. Small-molecule polyalcohol is added to serve as an inductive agent, the PVA degrading enzyme activity of crude enzyme produced by mixed flora is remarkably improved, the use amount of the needed crude enzyme in the process of degrading polyvinyl alcohol slurry is effectively reduced, and culture time and culture cost of preparation of the crude enzyme are saved; meanwhile, no expensive commercial PVA degrading enzyme needs to be purchased, and the method is suitable for industrial application. According to the method, the problems of flocculation and difficult separation of PVA degrading enzyme caused by a high-polymerization polyvinyl alcohol inductive agent are avoided.

Description

technical field [0001] The invention relates to a cotton fabric desizing method based on the compounding of crude enzyme liquid produced by mixed bacterial flora and amylase, and belongs to the technical field of textiles. Background technique [0002] In order to weave cotton and polyester-cotton fabrics smoothly, it is necessary to sizing the warp yarns of cotton and polyester-cotton fabrics to improve the wear resistance and strength of the warp yarns, and to fit the hairiness. The sizing material used is called sizing. The current sizing is mainly starch and PVA, and the sizing amount is generally 8%-12% of the warp quality, of which starch accounts for 70%-100% and PVA accounts for 0%-30%, depending on the variety. After weaving is completed, the slurry on it needs to be removed, otherwise it will affect the appearance and feel of the fabric and the subsequent dyeing, printing, finishing and other processes. This process is generally referred to as desizing. [0003] ...

Claims

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Application Information

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IPC IPC(8): D06L1/14D06M15/11D06M15/333C12N1/20C12N9/04C12N9/14D06M101/06C12R1/38
CPCC12N1/20C12N9/0006C12N9/14C12Y101/0303C12Y307/01007D06L1/14D06M15/11D06M15/333D06M2101/06D06M2200/50
Inventor 范雪荣张颖王强张洁王平袁久刚余圆圆崔莉
Owner JIANGNAN UNIV
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