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A method for degrading polyvinyl alcohol slurry by using mixed bacterial flora to produce crude enzyme solution

A technology of mixing flora and polyvinyl alcohol, applied in the textile field, can solve the problems of poor treatment effect and unstable PVA degrading enzyme effect, and achieve the effects of saving culture time, reducing COD value and improving treatment effect.

Active Publication Date: 2017-09-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing reported PVA carries out the enzymatic desizing method of the cotton fabric of simulated sizing process at present, mainly utilizes pure PVA to carry out simulated sizing to the fabric, and then uses commercial PVA degrading enzyme preparation to carry out its desizing, yet this report adopts simulated sizing, Whether it can really be used in industrial production is unknown, and the data show that it can only achieve better results under a certain implementation condition, which may be due to the unstable effect of the PVA degrading enzyme used
There are also many workers who have studied the combination of various enzymes for the desizing process of mixed pulp, but there is also the problem of poor treatment effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Crude enzyme solution produced by mixed flora

[0029] The mixed flora was cultivated on a broth medium to prepare a seed solution, and then inoculated into a broth medium, and fermented at 30°C for 3 days; then the cells were collected, broken walls, and centrifuged to obtain the supernatant as a crude enzyme solution.

[0030] The broth medium contains peptone 10.0-15.0 g / L, beef powder 3.0-5.0 g / L, sodium chloride 5.0-6.0 g / L, and pH 7.2±0.2. No enzyme activity was detected in the cell debris after fermentation.

Embodiment 2

[0031] Example 2: Adding inducer to induce enzyme production by mixed flora

[0032] The mixed flora is cultivated on a broth medium to prepare a seed solution, and then inoculated into a broth medium with 4-6g / L 1,3-butanediol, fermented at 30°C for 3 days; then the cells are collected , Broken the wall, centrifuge to get the supernatant as crude enzyme solution.

[0033] The broth medium contains peptone 10.0~15.0g / L, beef powder 3.0~5.0g / L, sodium chloride 5.0~6.0g / L, 1,3-butanediol 4-6g / L, pH 7.2 ±0.2.

[0034] The enzyme activity in the crude enzyme solution was determined, and the result showed that it could reach 1.985 U / mL.

Embodiment 3

[0035] Example 3: Using crude enzyme solution to degrade polyvinyl alcohol slurry

[0036] Cotton fabric sizing pure PVA size:

[0037] (1) Immersion treatment solution, 30°C, pH 7.0, bath ratio 1:30, immersion time 5h, the addition amount of crude enzyme solution is 25mL / L (final enzyme activity is about 50U / L);

[0038] (2) Wash in hot water at 90℃ for 5 minutes;

[0039] (3) Wash in cold water.

[0040] The sizing rate of the cotton fabric sizing with the pure PVA size is 8%.

[0041] The PVA used for sizing is PVA1799.

[0042] The results show that the desizing rate of PVA can reach 83.22%, and the degradation rate of PVA can reach 52.13%.

[0043] This may be due to the fact that the crude enzyme solution of the present invention contains PVA secondary alcohol oxidase and PVA β-diketohydrolase. These two enzymes have a synergistic effect on the cotton fabric sizing with PVA size, thereby increasing PVA desizing effect.

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PUM

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Abstract

The invention discloses a method for producing crude enzyme to degrade polyvinyl alcohol sizing by utilizing mixed flora and belongs to the technical field of textile. According to the method, 1,3-butanediol is utilized as an inductive agent to remarkably improve PVA degrading enzyme activity of crude enzyme produced by mixed flora, and then the crude enzyme is utilized for degrading the polyvinyl alcohol sizing. According to the method, the amount of crude enzyme needed when the polyvinyl alcohol sizing is degraded is effectively reduced, and time and cost are saved; meanwhile, the crude enzyme includes PVA secondary alcohol oxidase and PVA beta-diketone hydrolase, the two enzymes carry out desizing on cotton fabric sized with PVA sizing, and can have the synergistic effect, and the treatment effect of a single enzyme or a compound enzyme is effectively improved. By the adoption of the method, the desizing rate of PVA can reach 83.22%, and the PVA degradation rate can reach 52.13%.

Description

Technical field [0001] The invention relates to a method for degrading polyvinyl alcohol slurry by using a mixed flora to produce a crude enzyme solution, and belongs to the technical field of textiles. Background technique [0002] Polyvinyl alcohol (PVA) is a synthetic water-soluble polymer. Because of its excellent properties such as stable pH, good miscibility and strong emulsification ability, it also has good adhesion, film-forming and abrasion resistance to natural fibers and general synthetic fibers. It is currently used in the sizing of polyester-cotton fabric warp yarns. The main slurry. The fabric sizing with PVA must undergo desizing treatment in the pre-processing of textiles to increase its water absorption. [0003] For cotton and polyester-cotton fabrics containing PVA sizing agent, the current desizing still uses the chemical method of hot alkali treatment. This method only dissolves starch and PVA slurry without decomposing. Therefore, the COD value of desizing...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D06L1/14D06M15/333C12N9/04C12N9/14C12R1/01C12R1/38D06M101/06
CPCC12N9/0006C12N9/14C12Y101/03018D06L1/14D06M15/333D06M2101/06
Inventor 张颖范雪荣王强张洁王平袁久刚余圆圆崔莉
Owner JIANGNAN UNIV
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