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A kind of method that promotes microorganism to produce polyvinyl alcohol degrading enzyme

A polyvinyl alcohol and microorganism technology, applied in the textile field, can solve the problems of easy flocculation, affecting microorganisms, reducing enzyme production, etc., to avoid flocculation, improve the enzyme activity of PVA degrading enzymes, and solve separation difficulties.

Active Publication Date: 2019-01-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, when high-polymerization polyvinyl alcohol is used as an inducer, there is a shortcoming that high-polymerization polyvinyl alcohol is very easy to flocculate, which will affect the degradation of polyvinyl alcohol by microorganisms, and then lead to a decrease in enzyme production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Mixed flora produces crude enzyme solution (not induced)

[0028] The mixed flora was cultured on the broth medium to prepare the seed solution, and then inoculated in the broth medium, and fermented at 30°C for 3 days; then the cells were collected, the walls were broken, and the supernatant was centrifuged to obtain a crude enzyme solution.

[0029] The broth medium contains 10.0-15.0 g / L of peptone, 3.0-5.0 g / L of beef powder, 5.0-6.0 g / L of sodium chloride, and a pH of 7.2±0.2.

[0030] The enzyme activity was measured after the cells were collected and broken, and no PVA degrading enzyme activity was detected.

Embodiment 2

[0031] Example 2: Adding 1,3-butanediol inducer to induce mixed flora to produce enzymes

[0032] Cultivate the mixed flora on the broth medium to prepare the seed solution, then inoculate it in the broth medium induced by 4-6g / L 1,3-butanediol, and ferment at 30°C for 3 days; then collect The cells were broken, and the supernatant was centrifuged to obtain the crude enzyme solution.

[0033] The broth medium contains peptone 10.0g / L, beef powder 5.0g / L, sodium chloride 5.0g / L, 1,3-butanediol 4-6g / L, pH 7.2±0.2.

[0034] The enzyme activity in the crude enzyme solution was measured, and the result showed that it could reach 1.985U / mL.

Embodiment 3

[0035] Embodiment 3: adding propylene glycol inducer to induce mixed flora to produce enzyme

[0036] Cultivate the mixed flora on broth medium to prepare seed liquid, then inoculate in broth medium containing 2-5g / L propylene glycol, ferment at 30°C for 3 days, then collect cells, break the wall, and centrifuge The supernatant is the crude enzyme solution.

[0037] The broth medium contains peptone 15.0g / L, beef powder 3.0g / L, sodium chloride 6.0g / L, propylene glycol 2-5g / L, pH 7.2±0.2. The enzyme activity in the crude enzyme solution was measured, and the result showed that it could reach 1.32U / mL.

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PUM

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Abstract

The invention discloses a method for promoting microorganisms to produce polyvinyl alcohol (PVA) degrading enzyme, belonging to the technical field of spinning. By taking micromolecular polyhydric alcohol as an inducer, the enzyme activity of PVA degrading enzyme of crude enzyme produced by a mixed flora is remarkably improved, the use amount of crude enzyme needed when PVA slurry is degraded is effectively reduced, culture time and culture cost for preparing the crude enzyme are saved and lowered, and the method is suitable for being applied to industry. The method avoids the problems of flocculation caused by a high-polymerization-degree PVA inducer and PVA degrading enzyme separation difficulty.

Description

technical field [0001] The invention relates to a method for promoting microorganisms to produce polyvinyl alcohol degrading enzymes, belonging to the technical field of textiles. Background technique [0002] Polyvinyl alcohol (PVA) is a synthetic water-soluble polymer. Because of its excellent properties such as stable pH, good miscibility and strong emulsifying ability, it also has good adhesion, film forming and abrasion resistance to natural fibers and general synthetic fibers. main slurry. Fabrics with PVA sizing must undergo desizing treatment in the pre-textile treatment process to increase their water absorption. The traditional desizing method is to use 70-90°C hot water to elute the cotton fabric, and the eluted PVA is discharged with the wastewater. This not only consumes a lot of water and energy, but also easily damages the cotton fibers; what is more serious is that PVA has a large chemical oxygen consumption and poor biodegradability, causing serious pollu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/04C12N9/14D06M16/00D06L1/14D06M101/06
CPCC12N9/0006C12N9/14C12Y101/0303C12Y307/01007D06L1/14D06M16/003D06M2101/06
Inventor 张颖王强范雪荣张洁王平袁久刚余圆圆崔莉
Owner JIANGNAN UNIV
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