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A method for desizing cotton fabric based on the compounding of crude enzyme solution produced by mixed bacteria and amylase

A technology of mixed bacterial flora and crude enzyme solution, which is applied in the textile field, can solve the problems of low enzyme activity, few types and distributions, and difficult extraction, etc., and achieve the effect of soft hand feeling and saving cultivation time

Active Publication Date: 2017-12-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The key to realize the biodegradation of PVA is to be able to screen the microorganisms that can efficiently degrade PVA, but the types and distribution of PVA degrading bacteria are not many, the culture period is long, the enzyme activity is low, and the extraction is not easy. Important factors for actual production application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Mixed flora produces crude enzyme solution (not induced)

[0029] Cultivate the mixed flora on broth medium to prepare seed liquid, then inoculate in broth medium, and ferment at 30°C for 3 days; then collect the cells, break the wall, centrifuge to get the supernatant, and pass the supernatant through Filter through a microporous membrane (pore size 0.45um, diameter 50mm), and then dialyze with 0.1mol / L potassium phosphate buffer (pH8.0) for 24h to obtain the crude enzyme solution.

[0030] The broth medium contains 10.0-15.0 g / L of peptone, 3.0-5.0 g / L of beef powder, 5.0-6.0 g / L of sodium chloride, and a pH of 7.2±0.2.

[0031] It is determined that the crude enzyme solution has no PVA degrading enzyme activity.

Embodiment 2

[0032] Example 2: Adding a small molecule polyol inducer to induce mixed flora to produce enzymes

[0033]Cultivate the mixed flora on broth medium to prepare seed liquid, then inoculate in broth medium containing 4-6g / L 1,3-butanediol, ferment at 30°C for 3 days; then collect cells , Break the wall, centrifuge and take the supernatant to measure the enzyme activity.

[0034] The broth medium contains 10.0-15.0 g / L of peptone, 3.0-5.0 g / L of beef powder, 5.0-6.0 g / L of sodium chloride, 4-6 g / L of 1,3-butanediol, and pH 7.2 ±0.2.

[0035] The results showed that the total enzyme activity of the PVA degrading enzyme system could reach 1.985U / mL.

Embodiment 3

[0036] Embodiment 3: Cotton fabric desizing method based on mixed flora producing crude enzyme liquid and amylase compound

[0037] Enzymatic desizing of PVA-starch sized cotton fabrics.

[0038] (1) Soak unsized cotton fabric in PVA1799-starch solution (PVA concentration 30-50g / L, starch concentration 30-50g / L) for 2-10min, pad and then dry at 105°C After drying for 10 minutes, wash the sized fabric with water at room temperature to remove the PVA-starch size that has poor binding force with the surface of the fabric. The cotton cloth sized by this method has a PVA content of 2% and a starch content of 5.6%.

[0039] (2) When desizing, steam the sized cotton fabric for 40 minutes, and then immerse it in a phosphate buffer solution containing mixed bacterial flora producing crude enzyme liquid and amylase with a bath ratio of 1:10 (wherein the PVA degrading enzyme is present in the system). The final enzyme activity is 40U / L, the amylase activity is 6U / mL, pH7.0), the control...

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Abstract

The invention discloses a cotton fabric desizing method based on compounding of crude enzyme and amylase produced by mixed flora and belongs to the technical field of textile. Small-molecule polyalcohol is added to serve as an inductive agent, the PVA degrading enzyme activity of crude enzyme produced by mixed flora is remarkably improved, the use amount of the needed crude enzyme in the process of degrading polyvinyl alcohol slurry is effectively reduced, and culture time and culture cost of preparation of the crude enzyme are saved; meanwhile, no expensive commercial PVA degrading enzyme needs to be purchased, and the method is suitable for industrial application. According to the method, the problems of flocculation and difficult separation of PVA degrading enzyme caused by a high-polymerization polyvinyl alcohol inductive agent are avoided.

Description

technical field [0001] The invention relates to a cotton fabric desizing method based on the compounding of crude enzyme solution produced by mixed bacterial flora and amylase, and belongs to the technical field of textiles. Background technique [0002] In order to weave cotton and polyester-cotton fabrics smoothly, it is necessary to sizing the warp yarns of cotton and polyester-cotton fabrics to improve the wear resistance and strength of the warp yarns and to fit the hairiness. The sizing materials used are called sizing materials. The current sizing materials are mainly starch and PVA, and the sizing amount is generally 8%-12% of the warp yarn mass, of which starch accounts for 70%-100%, and PVA accounts for 0%-30%, depending on the variety. After the weaving is completed, the applied size needs to be removed, otherwise it will affect the appearance and feel of the fabric and the subsequent dyeing, printing, finishing and other processes. This process is generally call...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D06L1/14D06M15/11D06M15/333C12N1/20C12N9/04C12N9/14D06M101/06C12R1/38
CPCC12N1/20C12N9/0006C12N9/14C12Y101/0303C12Y307/01007D06L1/14D06M15/11D06M15/333D06M2101/06D06M2200/50
Inventor 范雪荣张颖王强张洁王平袁久刚余圆圆崔莉
Owner JIANGNAN UNIV
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