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Method for producing crude enzyme to degrade polyvinyl alcohol sizing by utilizing mixed flora

A technology of mixing bacteria and polyvinyl alcohol, applied in the textile field, can solve the problems of poor treatment effect and unstable PVA degrading enzyme effect, and achieve the effects of saving culture time, reducing COD value and protecting the ecological environment.

Active Publication Date: 2016-03-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing reported PVA carries out the enzymatic desizing method of the cotton fabric of simulated sizing process at present, mainly utilizes pure PVA to carry out simulated sizing to the fabric, and then uses commercial PVA degrading enzyme preparation to carry out its desizing, yet this report adopts simulated sizing, Whether it can really be used in industrial production is unknown, and the data show that it can only achieve better results under a certain implementation condition, which may be due to the unstable effect of the PVA degrading enzyme used
There are also many workers who have studied the combination of various enzymes for the desizing process of mixed pulp, but there is also the problem of poor treatment effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: mixed flora produces crude enzyme liquid

[0029] The mixed flora was cultured on the broth medium to prepare the seed solution, and then inoculated in the broth medium, and fermented at 30°C for 3 days; then the cells were collected, the walls were broken, and the supernatant was centrifuged to obtain a crude enzyme solution.

[0030] The broth medium contains 10.0-15.0 g / L of peptone, 3.0-5.0 g / L of beef powder, 5.0-6.0 g / L of sodium chloride, and a pH of 7.2±0.2. No enzyme activity was detected in the cell lysate after fermentation.

Embodiment 2

[0031] Example 2: Adding an inducer to induce mixed flora to produce enzymes

[0032] The mixed flora was cultured on the broth medium to prepare the seed solution, and then inoculated in the broth medium added with 4-6g / L 1,3-butanediol, and fermented at 30°C for 3 days; then the cells were collected, Break the wall, centrifuge and take the supernatant as crude enzyme solution.

[0033] The broth medium contains 10.0-15.0 g / L of peptone, 3.0-5.0 g / L of beef powder, 5.0-6.0 g / L of sodium chloride, 4-6 g / L of 1,3-butanediol, and pH7. 2±0.2.

[0034] The enzyme activity in the crude enzyme solution was measured, and the result showed that it could reach 1.985U / mL.

Embodiment 3

[0035]Example 3: Utilize crude enzyme solution to degrade polyvinyl alcohol slurry

[0036] Cotton fabrics sized with pure PVA size:

[0037] (1) Immersion treatment solution, 30°C, pH 7.0, bath ratio 1:30, immersion time 5h, in which the amount of crude enzyme solution added is 25mL / L (final enzyme activity is about 50U / L);

[0038] (2) Wash in hot water at 90°C for 5 minutes;

[0039] (3) Wash in cold water.

[0040] The sizing rate of the cotton fabric sized by the pure PVA size is 8%.

[0041] The PVA used for the sizing is PVA1799.

[0042] The results show that the desizing rate of PVA can reach 83.22%, and the degradation rate of PVA can reach 52.13%.

[0043] This may be due to containing PVA secondary alcohol oxidase and PVAβ-diketone hydrolase in the crude enzyme liquid of the present invention, these two kinds of enzymes have synergistic effect when desizing the cotton fabric of PVA size sizing, thereby improved PVA desizing effect.

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PUM

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Abstract

The invention discloses a method for producing crude enzyme to degrade polyvinyl alcohol sizing by utilizing mixed flora and belongs to the technical field of textile. According to the method, 1,3-butanediol is utilized as an inductive agent to remarkably improve PVA degrading enzyme activity of crude enzyme produced by mixed flora, and then the crude enzyme is utilized for degrading the polyvinyl alcohol sizing. According to the method, the amount of crude enzyme needed when the polyvinyl alcohol sizing is degraded is effectively reduced, and time and cost are saved; meanwhile, the crude enzyme includes PVA secondary alcohol oxidase and PVA beta-diketone hydrolase, the two enzymes carry out desizing on cotton fabric sized with PVA sizing, and can have the synergistic effect, and the treatment effect of a single enzyme or a compound enzyme is effectively improved. By the adoption of the method, the desizing rate of PVA can reach 83.22%, and the PVA degradation rate can reach 52.13%.

Description

technical field [0001] The invention relates to a method for degrading polyvinyl alcohol slurry by using a crude enzyme solution produced by mixed bacteria, belonging to the technical field of textiles. Background technique [0002] Polyvinyl alcohol (PVA) is a synthetic water-soluble polymer. Because of its excellent properties such as stable pH, good miscibility and strong emulsifying ability, it also has good adhesion, film forming and abrasion resistance to natural fibers and general synthetic fibers. main slurry. Fabrics with PVA sizing must undergo desizing treatment in the pre-textile treatment process to increase their water absorption. [0003] For cotton and polyester cotton fabrics containing PVA size, the current desizing still adopts the chemical method of hot alkali treatment. This method only dissolves the starch and PVA size without decomposing it. Therefore, the COD value of the desizing wastewater seriously exceeds the standard, which affects the ecologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06L1/14D06M15/333C12N9/04C12N9/14C12R1/01C12R1/38D06M101/06
CPCC12N9/0006C12N9/14C12Y101/03018D06L1/14D06M15/333D06M2101/06
Inventor 张颖范雪荣王强张洁王平袁久刚余圆圆崔莉
Owner JIANGNAN UNIV
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