In-situ microorganism restoring method for nitrophenol isomeride polluted environment and application
A technology of isomers and bioremediation, which is applied in the field of environmental organic pollutant treatment, can solve the problems of bioremediation without the three isomers of nitrophenol
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Embodiment 1
[0063] A method for utilizing microorganisms to carry out bioremediation of organic pollutants, the steps of which are:
[0064] A. Construction of microbial mixed flora:
[0065] Three kinds of microbial strains are cultured respectively, the preparation method of Alcaligenes sp.NyZ215 (CCTCC: preservation number M206121), Cupriavidus necator JMP134 (DSMZ: preservation number DSMZ4058), Pseudomonas sp.WBC-3 (CCTCC: preservation number M201027), comprises the following steps :
[0066] a. Preparation of inorganic salt medium: the composition is disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 14.3g, potassium dihydrogen phosphate (KH 2 PO 4 ) 3.0g, magnesium sulfate (MnSO 4 ·H 2 O) 0.28mg, ferrous sulfate (FeSO 4 ·7H 2 O) 0.3mg, magnesium sulfate (MgSO 4 ) 0.06mg, calcium chloride (CaCl 2 ) 1mg, copper sulfate (CuSO 4 ) 0.05mg, zinc sulfate (ZnSO 4 ) 0.05mg and boric acid (H 3 BO 3 ) 0.05mg, dilute it to 1000ml with double distilled water, adjust the pH to 7.0, ...
Embodiment 2
[0083] A kind of application utilizing microbial degrading flora in nitrophenol in-situ bioremediation (environmental pollution control), its steps are:
[0084] A. Construction of microbial mixed flora:
[0085] Three kinds of microbial strains are cultured respectively, the preparation method of Alcaligenes sp.NyZ215 (CCTCC: preservation number M206121), Cupriavidus necator JMP134 (DSMZ: preservation number DSMZ4058), Pseudomonas sp.WBC-3 (CCTCC: preservation number M201027), comprises the following steps :
[0086] a. Preparation of inorganic salt medium: the composition is disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O) 14.3g, potassium dihydrogen phosphate (KH 2 PO 4 ) 3.0g, magnesium sulfate (MnSO 4 ·H 2 O) 0.28mg, ferrous sulfate (FeSO 4 ·7H 2 O) 0.3mg, magnesium sulfate (MgSO 4 ) 0.06mg, calcium chloride (CaCl 2 ) 1mg, copper sulfate (CuSO 4 ) 0.05mg, zinc sulfate (ZnSO 4 ) 0.05mg and boric acid (H 3 BO 3 ) 0.05mg, dilute it to 1000ml with double distil...
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