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Aeromonas bestiarum having PHAs synthesizing performance

A technology of Aeromonas veterinosa and Aeromonas veterinary bacteria, which is applied in the field of environmental microorganisms, can solve the problems such as no reports on the synthesis of polyhydroxyalkanoate by Aeromonas veterinary bacteria, and achieve low production cost and easy fermentation and cultivation Effect

Active Publication Date: 2015-05-20
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the current research reports, there have been studies on polyhydroxyalkanoate synthetic strains, but there has been no report on the synthesis of polyhydroxyalkanoate by Aeromonas

Method used

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  • Aeromonas bestiarum having PHAs synthesizing performance
  • Aeromonas bestiarum having PHAs synthesizing performance
  • Aeromonas bestiarum having PHAs synthesizing performance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Screening of Aeromonas animal (Aeromonas bestiarum Ace-12) bacterial strain

[0023] 1. Strain and medium, culture conditions

[0024] (1) Bacterial source

[0025] The source of the bacteria comes from the activated sludge of the urban sewage treatment plant. The inoculum sludge used was the return sludge of Beijing Gaobeidian Sewage Treatment Plant, with sodium acetate, ammonium chloride, and dipotassium hydrogen phosphate as substrates, and the carbon, nitrogen, and phosphorus ratio was adjusted. Sequencing batch reactor (Sequencing Batch Reactor) was used to Reactor, SBR) domesticated activated sludge with high PHAs storage capacity. The effective volume of the reactor is 10L, and the instantaneous water intake mode is adopted, with 2L water intake in each cycle, and the operation is 12 hours, including 10 hours of aeration with a blower pump, 1.5 hours of sedimentation, 0.5 hours of drainage and water intake, and two cycles per day, continuous Accl...

Embodiment 2

[0039] Embodiment 2: Aeromonas animal (Aeromonas bestiarum Ace-12) synthesizes PHAs

[0040] 1. Carbon source and nitrogen source utilization experiments

[0041] The univariate method was used to study the effects of carbon source type and nitrogen source type on the aerobic synthesis of PHAs by the strains in the second step. Glucose, lactose, sucrose, glycerin, sodium acetate, sodium butyrate, ethanol, and soluble starch were used as carbon sources. When the single carbon source utilization experiment was performed on the identified Aeromonas veterinary bacteria, ammonium chloride, ammonium nitrate, sodium nitrate, Yeast powder, peptone, beef extract, ammonium sulfate, and urea were used as nitrogen sources, and a single nitrogen source utilization experiment was carried out for the identified Aeromonas veterinary bacteria.

[0042] All media contain KH 2 PO 4 2.65g / L, MgSO 4 ·7H 2 O 0.2g / L, Na 2 HPO 4 12H 2 O3.8g / L, ZnSO 4 ·7H 2 O 0.01g / L, CaCl 2 2H 2 O 0.05g / ...

Embodiment 3

[0061] Embodiment 3: Extraction and infrared detection of PHAs synthesized by Aeromonas animal (Aeromonas bestiarum Ace-12)

[0062] Take 1L of fermentation broth, centrifuge at 6000r / min at 4°C for 10min, discard the supernatant and add sodium hypochlorite to break the wall, centrifuge for 10min, discard the supernatant, wash with distilled water and centrifuge for 10min, discard the supernatant, add an appropriate amount of acetone and centrifuge for 10min, discard the supernatant For the clear solution, place the bacteria at a constant temperature of 60°C for 30 minutes, wait for the acetone to evaporate completely, add an appropriate amount of chloroform, shake well, put the chloroform that dissolves PHAs in a petri dish, keep the temperature at 70°C for 120 minutes, and then add the chloroform Volatilize completely to obtain PHAs film.

[0063] The extracted PHAs film is analyzed by infrared spectroscopy, and it is generally believed that the carbonyl peak is at 1724cm -...

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Abstract

The invention relates to aeromonas bestiarum having PHAs synthesizing performance and belongs to the field of environmental microorganisms. The aeromonas bestiarum strain is classified and named as aeromonas bestiarum and has the collection number of CGMCC No. 10332. The strain is obtained by screening from an acclimated activated sludge mixed flora system and can be applied in preparation of polyhydroxy alkanoates. By selecting fermentation synthesis conditions and adopting sodium acetate as a carbon source, the total amount of PHAs synthesized by the strain accounts for 63.21% of the dry weight of thalli.

Description

technical field [0001] The invention relates to an Aeromonas veterinosa capable of synthesizing PHAs, in particular to a Aeromonas veterinosa strain screened from activated sludge flora, capable of synthesizing PHAs with a carbon source of sodium acetate, and the synthesis amount reaches thallus 63.21% of the dry weight belongs to the field of environmental microorganisms. Background technique [0002] Polyhydroxyalkanoate (Polyhydroxyalkanoate, referred to as PHAs) is a kind of carbon source and energy storage accumulated by a class of cells when they grow under nutrient non-balanced conditions (excessive carbon source, while nitrogen source, phosphorus, magnesium, sulfur, etc. are relatively deficient). substance. In nature, microorganisms that can produce PHAs are widely distributed, including photo- and chemoautotrophic and heterotrophic bacteria, nearly 300 species of microorganisms, such as Actinomycetes, Alcaligenes, Pseudomonas, and Rhizobium Wait. [0003] Polyhy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/62C12R1/01
CPCC12P7/625C12N1/205C12R2001/01
Inventor 张艳萍仇少静汪苹董黎明秦清
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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