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Preparation method and application of immobilized bacteria for improving denitrification efficiency of constructed wetland

A technology of artificial wetland and denitrification efficiency, applied in chemical instruments and methods, fixed on/in organic carriers, aerobic process treatment, etc., can solve the problem of reducing cell activity, short service life, poor mass transfer performance, etc. problem, to achieve good biocompatibility, long service life and good stability

Inactive Publication Date: 2012-03-28
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since Robertson et al. (Robertson et al. Archives of Microbiology, 1984, 139, 351-354) first isolated aerobic denitrification bacteria in the desulfurization and denitrification treatment system in the 1980s, people have paid attention to aerobic denitrification The denitrification performance of bacteria has been studied to a certain extent, and some research has been carried out on the treatment of nitrogen-containing wastewater by embedding and immobilizing such bacteria, but the aerobic denitrifying bacteria after embedding and immobilization are applied to Not yet reported in wetland systems
[0003] At present, there have been many studies in the literature on the use of sodium alginate and polyvinyl alcohol as immobilized materials for denitrification, but for polyvinyl alcohol, a synthetic polymer gel carrier, it has good anti-microbial decomposition. High mechanical strength, good chemical stability, but poor mass transfer performance, which will reduce cell activity during the process of embedding cells; and sodium alginate, a natural polymer gel carrier, is non-toxic to microorganisms and has good mass transfer performance , but the strength is low, easy to be decomposed by microorganisms under anaerobic conditions, and the service life is short

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  • Preparation method and application of immobilized bacteria for improving denitrification efficiency of constructed wetland
  • Preparation method and application of immobilized bacteria for improving denitrification efficiency of constructed wetland
  • Preparation method and application of immobilized bacteria for improving denitrification efficiency of constructed wetland

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for preparing immobilized bacteria that improves nitrogen removal efficiency in constructed wetlands, the steps of which are:

[0033] 1) Screen the aerobic denitrifying strains from the aerobic composite vertical flow constructed wetland, and carry out screening and cultivation of the strains in an incubator with a constant temperature of 28°C;

[0034] The medium composition is: agar 20g, potassium nitrate 1g, potassium dihydrogen phosphate 1g, ferrous chloride hexahydrate 0.5g, calcium chloride heptahydrate 0.2g, magnesium sulfate heptahydrate 1g, sodium succinate 8.5g, bromine 1 mL of daphneol blue reagent; 1000 mL of distilled water, with 1 mol·L -1 Sodium hydroxide adjusted the pH to 7.0-7.3.

[0035] 2) Preparation of the bacterial suspension of aerobic denitrifying bacteria:

[0036] Select the aerobic denitrifying bacteria preserved on the slopes of 1-2 rings, add them to 750mL enrichment medium that has been sterilized by high temperature and pressu...

Embodiment 2

[0047] The same amount of three kinds of immobilized beads prepared in the preparation steps 1-5 in Example 1 were respectively placed in 0.01mol·L -1 , 0.1mol L -1 , 1mol L -1 HCl and NaOH, 1mol L -1 NaCl, CaCl 2 、KH 2 PO 4 Soak in the solution for 24 hours, and observe the changes in the integrity and strength of the immobilized pellets. The results are shown in Table 1, the sodium alginate-polyvinyl alcohol immobilized beads have better stability in acid-base salt solution.

[0048] Table 1 Stability of three kinds of carrier-embedded immobilized beads in acid, alkali and salt solution

[0049]

[0050] Note: no change (0); slightly softer, slightly foamy, poorer elasticity (+); softer, foamy, poorer elasticity (++); foaming, loose structure, brittle (+++)

[0051] Other steps are the same as in Example 1.

Embodiment 3

[0053] An application of an immobilized bacterium improving the denitrification efficiency of a constructed wetland in a constructed wetland, the steps of which are:

[0054] The sodium alginate-polyvinyl alcohol immobilized pellets prepared in the preparation steps 1-5 in Example 1 were applied to the denitrification research in the simulated column of the constructed wetland. The concentration of nitrate nitrogen wastewater for denitrification treatment is 80mg / L, COD Cr About 400mg / L. The filling height is about 2.5cm, and the device such as figure 2 As shown, among them, the lower layers of A and B systems are filled with 2-3 mm round ceramsite, and the upper layers are respectively immobilized pellets and ceramsite soaked by bacterial suspension. , the same specifications of ceramsite, respectively, 4h, 8h, 12h, 18h, 24h, 36h, 48h, 72h water samples were taken to analyze the nitrification rate and nitrite nitrogen residue, the results are as follows image 3 , 4 show...

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Abstract

The invention discloses a preparation method and an application of immobilized bacteria for improving the denitrification efficiency of a constructed wetland. 1. The preparation steps are as follows: 1) screening and culturing aerobic denitrification strains which are screened out of the constructed wetland in a thermotank; 2) selecting aerobic denitrification bacteria in slope conservation, inoculating into a sterile enrichment culture medium and aerating to get bacterial suspension; 3) using sodium alginate-polyvinyl alcohol to embed so as to get the immobilized aerobic denitrification bacteria; and 4) taking pellets of the aerobic denitrification bacteria out of a refrigerator, washing with double distilled water or physiological saline, soaking in the physiological saline, and aerating to activate the immobilized pellets. 2. An application of the immobilized bacteria in the constructed wetland is as follows: filling the immobilized pellets into a simulation column of the constructed wetland for performing waste water denitrification treatment. The preparation method of the immobilized bacteria is simple, the production cost is low, the application in the industrial waste water treatment is easy, the denitrification efficiency is high, the accumulation amount of nitrite nitrogen is low, and the service life is long.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a method for preparing immobilized bacteria for improving nitrogen removal efficiency in constructed wetlands and an application of prepared immobilized microspheres in constructed wetlands. Background technique [0002] With the rapid development of industry and agriculture, the discharge of industrial "three wastes" has increased, domestic sewage, medical sewage, domestic garbage, and large-scale use of chemical fertilizers and pesticides in agriculture have caused water resources to be polluted to varying degrees, among which nitrate pollution is the main pollution type of water resources one. In recent years, constructed wetlands have played an important role in sewage treatment. Substrate, higher plants, and microorganisms are the three main factors for the purification of constructed wetlands. Many studies have shown that the primary factor for nitrogen remova...

Claims

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Application Information

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IPC IPC(8): C12N11/08C12N11/04C02F3/02C02F3/34
CPCY02W10/10
Inventor 吴振斌周巧红张燕王莹贺锋徐栋
Owner INST OF AQUATIC LIFE ACAD SINICA
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