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Bio-control agent for preventing and controlling root-knot nematodes, and application thereof

A bio-control fungicide, root-knot nematode technology, applied in the directions of nematicides, application, biocides, etc., can solve the problems of low control effect and single disease prevention mechanism, and achieve the promotion of quantity, broad application prospects, and improvement of plant resistance. disease effects

Active Publication Date: 2019-01-11
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of single disease prevention mechanism and low control effect of control plants based on a single strain of nematode, the present invention provides a biocontrol agent for controlling root-knot nematode and its application

Method used

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  • Bio-control agent for preventing and controlling root-knot nematodes, and application thereof
  • Bio-control agent for preventing and controlling root-knot nematodes, and application thereof

Examples

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Effect test

Embodiment 1

[0023] Embodiment 1: a kind of anti-root-knot nematode biocontrol bacterium, consists of bacillus licheniformis ( Bacillus licheniformis ) - Pseudomonas fluorescens ( Pseudomonas fluorescens ) mixed fermentation broth, Streptomyces freundii ( Streptomyces fradiae ) fermentation broth, Phanerochaete chrysosporium ( Phanerochaete chrysosporium Burdsall ) - Myrocystis verrucosus ( Myrothecium verrucaria ) The mixed fermentation liquid was mixed according to the volume ratio of 2:2:6, and after stirring for 10 minutes, the liquid bacterial agent was obtained; the liquid bacterial agent and quinoa husk were mixed evenly at 1kg:6kg, dried at 35°C for 48 hours, and crushed at 200 meshes to prevent and control Root-knot nematode biocontrol agent.

[0024] Of which: Bacillus licheniformis ( Bacillus licheniformis ) - Pseudomonas fluorescens ( Pseudomonas fluorescens ) The mixed fermentation liquid is: Bacillus licheniformis seed liquid and Pseudomonas fluorescens seed...

Embodiment 2

[0030] Embodiment 2: a kind of anti-root-knot nematode biocontrol bacterium, consists of bacillus licheniformis ( Bacillus licheniformis ) - Pseudomonas fluorescens ( Pseudomonas fluorescens ) mixed fermentation broth, Streptomyces freundii ( Streptomyces fradiae ) fermentation broth, Phanerochaete chrysosporium ( Phanerochaete chrysosporium Burdsall ) - Myrocystis verrucosus ( Myrothecium verrucaria ) The mixed fermentation broth was mixed according to the volume ratio of 3:1:7, and after stirring for 10 minutes, the liquid bacterial agent was obtained; the liquid bacterial agent and quinoa husk were mixed evenly at 1kg:8kg, dried at 35°C for 48 hours, and crushed at 200 meshes to prevent and control Root-knot nematode biocontrol agent.

[0031] Of which: Bacillus licheniformis ( Bacillus licheniformis ) - Pseudomonas fluorescens ( Pseudomonas fluorescens ) The mixed fermentation liquid is: Bacillus licheniformis seed liquid and Pseudomonas fluorescens seed ...

Embodiment 3

[0037] Embodiment 3: a kind of anti-root-knot nematode biocontrol bacterium is made of bacillus licheniformis ( Bacillus licheniformis ) - Pseudomonas fluorescens ( Pseudomonas fluorescens ) mixed fermentation broth, Streptomyces freundii ( Streptomyces fradiae ) fermentation broth, Phanerochaete chrysosporium ( Phanerochaete chrysosporium Burdsall ) - Myrocystis verrucosus ( Myrothecium verrucaria ) The mixed fermentation broth was mixed according to the volume ratio of 5:3:1, and after stirring for 10 minutes, the liquid bacterial agent was obtained; the liquid bacterial agent and quinoa husks were mixed evenly at 1kg:7kg, dried at 35°C for 48 hours, and crushed at 200 meshes to prevent and control Root-knot nematode biocontrol agent.

[0038] Of which: Bacillus licheniformis ( Bacillus licheniformis ) - Pseudomonas fluorescens ( Pseudomonas fluorescens ) The mixed fermentation liquid is: Bacillus licheniformis seed liquid and Pseudomonas fluorescens seed l...

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Abstract

The invention belongs to the technical field of microbial pesticides, and provides a bio-control agent for preventing and controlling root-knot nematodes, and an application thereof in order to solvethe problems of single disease prevention mechanism and low prevention and control effect of existing single strains for preventing and controlling root-knot nematodes. The bio-control agent is prepared by mixing a Bacillus licheniformis-Pseudomonas fluorescens mixed fermentation broth, a streptomyces fradiae fermentation broth and a Phanerochaete chrysosporium-Myrothecium verrucaria mixed fermentation broth according to a volume ratio of (2-5):(1-3):(1-7), and uniformly mixing the obtained mixed solution with quinoa bran according to a mass ratio of 1 kg:(6-8) kg. The above five strains do not antagonize each other and cooperate with each other to kill insects, and the preparation method is simple, so the bio-control agent has a broad application prospect. The bio-control agent can prevent and control root-knot nematodes, promote the growth and development of plants and improve the disease resistance of plants.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a biocontrol agent for preventing and controlling root-knot nematodes and an application thereof. Background technique [0002] root-knot nematodes ( Meloidogyne spp. ) disease is one of the important root diseases that seriously affects the yield of vegetable protected cultivation in the world. It has the characteristics of wide distribution, multi-host, strong infectivity and hidden damage. With the rapid development of protected vegetable cultivation, the incidence of root-knot nematodes is increasing year by year, and severe areas can reduce production by 30-40%, or even fail. At present, the most important means of prevention and control in agriculture are still pesticides and soil disinfection, resulting in a large number of pesticide residues and environmental pollution. Therefore, improving the quality and yield of vegetables and seeking safe, e...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N63/04A01P5/00
CPCA01N63/00A01N63/30
Inventor 董海龙路平杜宾王琳徐玉梅张作刚崔克勇王建明王慧杨斌
Owner SHANXI AGRI UNIV
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