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Bacillus methylotrophicus Sanju-04 and its application

A methylotrophic and Bacillus technology, applied in the field of plant disease prevention and control, to achieve the effect of convenient application, high cost performance, and broad application prospects

Inactive Publication Date: 2013-05-01
FUJIAN SANJU BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is far from developed countries in terms of variety, dosage form, quality and quantity, and is still far from meeting the needs of domestic and foreign markets.

Method used

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  • Bacillus methylotrophicus Sanju-04 and its application
  • Bacillus methylotrophicus Sanju-04 and its application
  • Bacillus methylotrophicus Sanju-04 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 strain isolation and identification

[0019] Isolation of Bacillus methylotrophicus Sanju-04CCTCC No.M2012169: collected from Liudou Mountain, Letu Village, Hexi Town, Nanjing County, Fujian Province (117°1ˊ30" east longitude, 24°56ˊ20" north latitude, 360 meters above sea level ) soil, from which a methylotrophic Bacillus strain was isolated by nutrient agar plate, named Sanju-04, which was preserved in the Chinese Center for Type Culture Collection of Wuhan University on May 16, 2012. No. CCTCC No. M2012169. After identification, the bacterium is a methylotrophic bacillus, and has the following characteristics: a single cell is 0.7-0.8×2-3 microns, and the coloring is uniform. Uncapsulated, with periosteum flagella, able to move. Gram-positive bacteria, spores 0.6-0.9×1.0-1.5 microns, elliptical to columnar, located in the center of the bacterium or slightly biased, the bacterium does not expand after spore formation. The surface of the colony is rough an...

Embodiment 2

[0059] Embodiment 2 bacterial agent preparation

[0060] (1) Culture medium: ① Incline medium: 5.0g peptone, 3.0g beef extract, 250ml soil extract, 18g agar, 750ml distilled water, pH7.2-7.5; (soil extract: 50g soil, add 200ml water, sterilize for 1h, Filter water to 250ml.) ②Liquid seed medium: glucose 1.2%, yeast extract 0.75%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, calcium carbonate 0.08%, pH7.2; ③Liquid fermentation medium: sucrose 1.0 %, yeast extract 0.65%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.05%, sodium chloride 0.02%, calcium carbonate 0.05%, pH7.2.

[0061] (2) Production slant preparation: After sterilizing the prepared slant medium in a sterilizing pot at 121°C for 25 minutes, take it out and place it on a slant to cool to room temperature, take out the preserved slant, transfer the tube in the sterile room, 1 preserved slant Transfer to three production slopes, place the inoculated production slopes in an incubator at a...

Embodiment 3

[0066] Embodiment 3 is to capsicum growth-promoting effect experiment

[0067] Take 60 pepper seedlings with the same growth status and health level, and set three treatments when transplanting: adding solid bacterial agent as base fertilizer (one application), liquid bacterial agent mixed with water 400 times to irrigate roots (three applications) and no additional bacterial agent The agent was used as the base fertilizer, and the water was used as the control, and other cultivation and management measures were the same. 30d Investigate the effect of promoting the growth of pepper plants.

[0068] The influence of table 1 bacterial agent on pepper growth

[0069] item (processing) Solid bacteria Liquid bacteria Ck (clear water) Plant height per plant (cm) 79.21 73.58 66.15 Fresh weight of aerial parts of a single plant (g) 29.66 28.72 20.60 Root fresh weight per plant (g) 1.90 1.77 1.02

[0070] It can be seen from Table 1 that th...

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Abstract

The invention relates to a Bacillus methylotrophicus Sanju-04 and its application. The above strain is preserved at China Center for Type Culture Collection (CCTCC) in Wuhan University, has a preservation date of May, 16, 2012, and has a preservation number of CCTCC No:M2012169. The Bacillus methylotrophicus Sanju-04 has very good capsicum bacterial wilt and orange anthracnose control efficacies, and has the advantages of stability, no toxic chemical substance residual, no pollution to agricultural products or the environment, high cost performance, and application convenience. The Bacillus methylotrophicus Sanju-04 plays an important role in the control of the bacterial wilt and the anthracnose of plants, and has a wide application prospect.

Description

technical field [0001] The present invention relates to a strain of methylotrophic bacillus and its specific application, in particular to a strain of methylotrophic bacillus (Bacillus methylotrophicus) Triju-04 and its bacterial agent and the application of the bacterial agent in the prevention and treatment of plant diseases . Background technique [0002] Bacterial wilt is a worldwide disease widely distributed in tropical, subtropical and some temperate regions, and is the main cause of crop yield reduction. In my country, Fujian, Guangdong, Guangxi, Sichuan, Yunnan, Hunan, Jiangxi, Zhejiang, Shanghai , Jiangsu, Anhui and other provinces, cities (districts) are distributed. Pepper bacterial wilt is one of the most serious soil-borne diseases in the pepper planting process. Due to the rapid development of spring early maturity and autumn delayed cultivation of pepper in plastic greenhouses in recent years, pepper bacterial wilt is gradually increasing. Spring early-matur...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/07
Inventor 林克明尤越
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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