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Integrated kit for in-situ molecular diagnosis and molecular diagnosis method using same

A molecular diagnostics, kit technology, applied in the direction of chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of time-consuming and high cost, achieve low-contamination operation, cost-effective effect

Pending Publication Date: 2022-06-21
菲尔梅迪株式会社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, laboratory tests and methods produce highly accurate results but are time consuming and costly

Method used

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  • Integrated kit for in-situ molecular diagnosis and molecular diagnosis method using same
  • Integrated kit for in-situ molecular diagnosis and molecular diagnosis method using same
  • Integrated kit for in-situ molecular diagnosis and molecular diagnosis method using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1: All-in-one kit for on-site molecular diagnostics

[0090] refer to figure 1 and figure 2 , manufactured an integrated kit 10 for on-site molecular diagnosis, the kit includes: a first assembly 10A, the first assembly 10A includes a sample pretreatment part 100, a nucleic acid absorption part 200 and a nucleic acid amplification part 300; the second assembly 10B, the second assembly 10B includes the detection part 400; the third assembly 10C, the third assembly 10C includes two needleless connector lower parts 500A; the fourth assembly 10D, the fourth assembly 10D includes two needleless Connector upper portion 500B; thermal pack 10E; and sample collection tool 10F.

[0091] refer to Figure 4 and Image 6 , the sample pretreatment part 100 includes a transfer member 110 for transferring a lysis buffer in which nucleic acid is dissolved, and preprocesses the sample. The nucleic acid absorption part 200 is positioned on the sample pretreatment part 100 ...

Embodiment 2

[0094] Example 2: Molecular diagnostics after forming an assembly of an all-in-one kit for on-site molecular diagnostics

[0095] refer to Figure 8 , using the all-in-one kit for on-site molecular diagnosis manufactured according to Example 1 for molecular diagnosis. Molecular diagnostic methods are as follows:

[0096] First, by combining the first component 10A and the second component 10B of the all-in-one kit 10 for on-site molecular diagnosis to form the image 3 assembly shown in . Here, the needleless connector 500 is formed by combining the third component 10C and the fourth component 10D.

[0097] Then, by physically breaking the upper end of the lysis buffer chamber 120 with the sample collection tool 10F that has collected the nucleic acid-containing sample and then immersing the sample in the lysis buffer, a lysis buffer in which the nucleic acid is dissolved is generated.

[0098] Then, by physically breaking the lower end of the lysis buffer chamber 120 with...

Embodiment 3

[0103] Example 3: Formation of an assembly of an all-in-one kit for on-site molecular diagnostics during molecular diagnostics

[0104] refer to Figure 9 , using the all-in-one kit for on-site molecular diagnosis manufactured according to Example 1 for molecular diagnosis. Molecular diagnostic methods are as follows:

[0105] First, by physically breaking the upper end of the lysis buffer chamber 120 of the first assembly 10A with the sample collection tool 10F that has collected the nucleic acid-containing sample and then immersing the sample in the lysis buffer, a lysis buffer in which the nucleic acid is dissolved is generated.

[0106] Then, by physically breaking the lower end of the lysis buffer chamber 120 with the sample collection tool 10F, the lysis buffer in which the nucleic acid is dissolved is moved to the transfer member 110 of the sample pretreatment section 100, and the nucleic acid transferred along the transfer member 110 is bound to the Nucleic acid ab...

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Abstract

Proposed are an integrated kit for in-situ molecular diagnosis and a molecular diagnosis method using the same. The integrated kit for on-site molecular diagnosis comprises a first assembly and a second assembly, the first assembly comprises a sample pretreatment part, a nucleic acid absorption part and a nucleic acid amplification part, and the second assembly comprises a detection part. The sample pretreatment section includes a transfer member configured to transfer a lysis buffer in which nucleic acid is dissolved, and is configured to pretreat a sample. The nucleic acid absorption portion is positioned on the sample pretreatment portion and configured to be slidable onto the nucleic acid amplification portion. The nucleic acid amplification part is connected to the sample pretreatment part, and is configured to elute the nucleic acid from the nucleic acid absorption part and amplify the eluted nucleic acid. The detection section is configured to transfer the nucleic acid amplified by the nucleic acid amplification section and detect the nucleic acid. The integrated kit for in-situ molecular diagnosis and the molecular diagnosis method using the same according to the present disclosure are advantageous in that the kit enables ordinary people to perform molecular diagnosis without specialized devices (such as a centrifuge or a pipettor), ensures low-pollution operation, and has cost effectiveness.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS [0002] This application claims priority from Korean Patent Application Nos. 10-2020-0177139 filed on December 17, 2020 and 10-2021-0134058 filed on October 8, 2021, and is hereby incorporated by reference for all purposes Its entire content is incorporated herein. technical field [0003] The present disclosure relates to kits for molecular diagnostics and molecular diagnostic methods using the same, and more particularly, to an all-in-one kit for on-site molecular diagnostics and using the same of molecular diagnostic methods. Background technique [0004] In the United States, more than 1 billion infections occur each year, and many cases are mishandled due to inaccurate or delayed diagnoses. Many point-of-care (POC) tests suffer from low sensitivity (30% to 70%), while more sensitive tests (such as nucleic acid specific detection or molecular detection associated with pathogenic targets) only Available in the lab. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/02C12M1/34C12M1/38C12M1/00C12Q1/6844C12Q1/70C12Q1/689C12R1/93C12R1/32
CPCC12Q1/6844C12Q1/703C12Q1/701C12Q1/708C12Q1/689C12Q2523/308C12Q2527/125C12Q2565/625B01L2200/026B01L2200/0621B01L2200/16B01L2300/044B01L3/5029B01L2300/0816B01L2300/087B01L2300/0825B01L2300/069B01L2400/065B01L2400/0683B01L1/52B01L7/52B01L2300/1855B01L2200/10B01L3/5023B01L3/523B01L7/00B01L2300/18
Inventor 李柄焕金世镇宋隼圭
Owner 菲尔梅迪株式会社
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