Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immunosensor for detecting ZEN (zearalenone) and preparation method thereof

A technology of zearalenone and immunosensor, which is applied in the field of testing or analyzing material safety, can solve the problems of inaccuracy and reliability, large subjective influence, and prone to false positives, etc., and achieves strong practicability, simple synthesis method, and wide application range wide effect

Active Publication Date: 2018-09-28
SHANGHAI ACAD OF AGRI SCI
View PDF1 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the preliminary investigation and research, it was found that 3 parts of the blood of 260 normal people contained ZEN, and 18 parts of their urine contained ZEN, which caused huge potential risks to human health.
[0003] At present, there are mainly two methods for risk assessment of ZEN: one is to measure the content of ZEN in cereals and other foods and combine consumer dietary surveys to obtain the exposure of human pollutants. This assessment method is simple, but due to the variety of sources of ZEN, Including different food, air, water, skin contact, etc., so this method is generally not accurate and reliable; the second is to measure the content of ZEN in blood and urine, and calculate the exposure of pollutants through the human metabolic coefficient
The second method is accurate and reliable, but because the content of ZEN in blood and urine is very low, generally at the ng level, the accuracy, sensitivity and reliability of the detection technology become the bottleneck of this risk assessment method
Thin-layer chromatography is low in cost and easy to operate, but semi-quantitative visual inspection is greatly affected by subjectivity; ELISA has strong specificity, simple sample pretreatment, and short analysis time, but the enzyme activity is easily affected by operating conditions and prone to occurrence False positive; high performance liquid chromatography and mass spectrometry have good sensitivity, accuracy and stability, and are suitable for various samples containing complex components. However, this method is only common in scientific research institutes and testing institutions, and is not suitable for grassroots application and promotion, and requires complex pre-processing methods to ensure the accuracy of results, and cannot achieve rapid detection and on-site instant analysis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunosensor for detecting ZEN (zearalenone) and preparation method thereof
  • Immunosensor for detecting ZEN (zearalenone) and preparation method thereof
  • Immunosensor for detecting ZEN (zearalenone) and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Preparation of immunosensor

[0053] The first step is to synthesize the dispersion of molybdenum disulfide and thionine complex:

[0054] Add 15 mg of thionine and 10 mg of molybdenum disulfide powder to 1 mL of N,N-dimethylformamide (DMF), and sonicate for 4 hours at room temperature. After centrifugation at 2000rpm for 20min, collect the upper layer liquid, centrifuge at 6000rpm for 20min, carefully wash the collected solid with DMF until almost colorless, dry it with nitrogen at 50°C, and redisperse in DMF with a concentration of 2mg / mL;

[0055] The second step is to synthesize ZEN monoclonal antibody modified with platinum nanoparticles:

[0056] Dilute the ZEN monoclonal antibody to 10 μg / mL with 0.01mol / L phosphate buffer (pH 7.4); slowly add 16 μL of chloroplatinic acid (20 mmol / L) to 360 μL of the diluted antibody under stirring conditions for 30 seconds Add 8 μL of sodium hydroxide solution (0.5 mol / L).

[0057] Stir for 13 hours under sealed and dark ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses an immunosensor for detecting ZEN (zearalenone). The immunosensor is prepared by a method with the following steps of (1) pretreatment of a glassy carbon electrode: polishing the glassy carbon electrode by aluminum oxide powder into a mirror surface, sequentially cleaning in anhydrous ethyl alcohol and acetone by ultrasonic waves, and airing; (2) treatment of the glassy carbon electrode: dripping dispersion liquid of molybdenum disulfide and thionine compound onto the glassy carbon electrode, and airing; then, dripping a platinum nanoparticle-modified ZEN monoclonal antibody solution, and airing; soaking in a 0.5% to 10% bovine serum albumin solution, and sealing, so as to obtain the required immunosensor. The immunosensor has the advantages that the sensitivity andrapidity effects are realized, and the application range is broad; the immunosensor is suitable for quickly detecting ZEN in biological samples of blood, urine and the like, and can be applied to hospitals and physical examination organizations, so as to provide quick and reliable basis for the judging of ZEN-related diseases.

Description

technical field [0001] The invention relates to the technical field of testing or analyzing material safety, in particular to an immunosensor for detecting zearalenone and a preparation method thereof. Background technique [0002] Zearalenone (ZEN) is a dihydroxybenzoic acid lactone metabolite with estrogen activity produced by a variety of Fusarium species, which is widely found in corn, wheat, sorghum, rice and other cereals. The structure of ZEN is similar to that of endogenous estrogen, and it can specifically bind to estrogen receptors, thereby inducing a series of reproductive toxicity and teratogenic effects; it can also cause lipid peroxidation in the body, causing damage to the liver and kidneys; In addition, ZEN also has certain toxic effects such as carcinogenicity, immunotoxicity, and teratogenicity. ZEN is stable in nature and is not easy to decompose during food storage, processing and cooking, so it is easy to remain in various processed raw materials. After...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 韩铮聂冬霞赵志辉范凯蒋可秋黄晴雯
Owner SHANGHAI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com