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ELISA for VEGF

A technology for capturing reagents, biological samples, applied in the field of immunoassays, which can solve the problems of lack of sensitivity

Active Publication Date: 2009-09-02
GENENTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While this ELISA is capable of detecting elevated VEGF levels in cancer patients, it lacks the sensitivity required to measure endogenous levels of VEGF in normal individuals

Method used

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  • ELISA for VEGF
  • ELISA for VEGF
  • ELISA for VEGF

Examples

Experimental program
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Embodiment 1

[0110] Vascular endothelial growth factor (VEGF), which is expressed in different isoforms due to alternative RNA splicing, is known to play a key role in tumor angiogenesis. We measured VEGF 165 and total VEGF concentrations, and assessed the VEGF 110 (which is the relative amount of the active fragment produced by plasmin digestion of VEGF). ELISA A (VEGF165-206 ELISA) detects VEGF 165 and longer isoforms but not VEGF 121 . ELISA B (VEGF110-206ELISA) detects VEGF 165 and isoforms VEGF 121 and VEGF 110 . ELISA C (VEGF121-206ELISA) detection of VEGF 165 and the longer isoform, VEGF 121 and molecular weight greater than VEGF 110 but not VEGF 110 Fragments of VEGF (referred to herein as "VEGF 110+ ").

[0111] Materials and methods

[0112] Reagents and cells: Recombinant VEGF 165 (Genentech), VEGF 121 (PeproTech, Rocky Hill, New Jersey), VEGF 8-109 (by VEGF 165 amino acid 8-109 composition) and truncated VEGF 121 (R&D Systems, Minneapolis, MN) produced in E. ...

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Abstract

The vascular endothelial growth factor (VEGF) activity in a patient's bloodstream or other biological sample can serve as a diagnostic and prognostic index for cancer, diabetes, heart conditions, and other pathologies. Antibody-sandwich ELISA methods and kits for VEGF as an antigen are provided to detect types of VEGF levels in biological samples from animal models and human patients and can be used as a diagnostic / prognostic index.

Description

[0001] related application [0002] This application claims priority and benefit to U.S. Provisional Application Serial No. 60 / 828,203, filed October 4, 2006, the entirety of which is incorporated herein into its specification. field of invention [0003] The present invention relates to immunoassays for the detection of certain VEGF populations, which can be used as diagnostic and prognostic methods in patients with cancer, cardiovascular, or other pathologies. Background of the invention [0004] It is now well established that angiogenesis is involved in the pathogenesis of a variety of disorders. These include solid tumors, intraocular neovascular syndromes such as proliferative retinopathy or age-related macular degeneration (AMD), rheumatoid arthritis, and psoriasis (Folkman et al., J. Biol. Chem .267: 10931-10934 (1992); Klagsbrun et al., Annu. Rev. Physiol. 53:217-239 (1991); and Garner A, Vascular diseases. In: Pathobiology of ocular disease.A dynamic approac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74C07K16/22
CPCC07K16/22G01N33/74G01N33/57496G01N2333/475
Inventor 玉茹·G·孟洪圭熙约翰尼·古铁雷斯
Owner GENENTECH INC
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