The invention relates to a method for producing glycyrrhetinic acid monoglucuronide (GAMG) through intermittent feed supplement
fermentation, belonging to the technical field of biological transformation. The method specifically comprises the steps of: firstly, cultivating and inducing microorganisms capable of inducing to generate beta-
glucuronidase in a seed culture medium and a
fermentation culture medium containing glycyrrhizic acid to obtain corresponding beta-
glucuronidase; and secondly, specifically splitting a terminal glucosidic bond in a glycyrrhizic acid molecular structure by using the beta-
glucuronidase generated by the microorganisms through induction, generating the GAMG, and secreting the GAMG to a
fermentation solution and intermittently replenishing glycyrrhizic acid and
inorganic salts as a substrate for multiple times. Through replenishing the glycyrrhizic acid and the
inorganic salts as the substrate,
nutrient substances are replenished,
thallus can grow in a proper environment and promote the synthesis of a target product; and after the fermentation is ended, the yield of the GAMG reaches 13-24g / L. According to the method disclosed by the invention, the fermentation yield of the GAMG can be effectively increased, the transformation period is prolonged, the equipment
utilization rate is increased, and the product production cost is reduced.