Artificial eel propagation method
A technology of artificial breeding and river eel, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of lack, low fertilization rate, and inability to meet mass production, so as to improve the level, reduce the dose of hormone injection, prevent Effects of anesthetics and procedural injuries
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Embodiment 1
[0022] Embodiment 1 broodstock ripening
[0023] Environmental factors: salinity 17-30‰, water temperature 16°C-24°C, pH 7.5-8.2, dissolved oxygen 4-15mg / L, stocking density 50 fish / pond, separate male and female.
[0024] The acclimatization period was carried out on the received parent river eel. Put the collected parent river eel into a cement pool (10m×3m×1.2m) with a half-shade cloth greenhouse, domesticate for 7 days to adapt to the artificial environment, introduce natural seawater (17‰) filtered by sedimentation, and use artificial sea salt Adjust to make the salinity of the pool water gradually rise to 20‰, and then every half month as a cycle, the salinity is increased by 3-4‰ until the salinity rises to 30-35‰. Add tiles, PVR tubes and other coverings under the sunshade net at the bottom of the pool. During the day, the fish hide in the covering, and at night they will move around in the area without the sunshade net, so as to avoid light during the day and see t...
Embodiment 2
[0026] Example 2 Broodstock induce labor
[0027] Environmental factors: salinity 30-35‰, water temperature 19.5-22.5°C, pH 7.0-8.0, dissolved oxygen 5-15mg / L, stocking density 12 fish / pond, male to female ratio 1:3.
[0028] After 2-3 weeks, the female eels that have begun to develop use their own reproductive regulation to reach the final water and action stage of ovary development. According to the state of the genital opening of the female eel (protruding and a little red and swollen), and the degree of change in abdominal circumference (appropriately enlarged but not inflated), the fish body is selected. The female eel is injected with the last injection of ripening agent in the morning of the first day, and injected with induced labor 24 hours later. The oxytocic agent is 17α-hydroxyprogesterone (OHP) (5mg / 500g·BW) mixed with HCG (150IU / 500g·BW). And select the male eels that can produce sperm, and inject a shot of CPE (0.5mg / 500g·BW) mixed with HCG (150IU / 500g·BW). ...
Embodiment 3
[0031] Embodiment 3 fertilized egg hatching
[0032]Environmental factors: strictly control salinity 33±0.5‰, water temperature 21±0.5, pH 7.2-7.5, dissolved oxygen above 6mg / L, ammonia and nitrogen content not exceeding 0.02mg / L and 0.05mg / L, Ca 2+ , Mg 2+ The mass concentration range is 325-330 mg / L, 1160-1165 mg / L.
[0033] The obtained fertilized eggs are immediately transferred to a special 50L circular cone-bottomed incubator for incubation. A small amount of aeration ensures sufficient dissolved oxygen, and the formation of a micro-water flow can drive the eggs to float. Hatching density: 600-800 grains / L. Change the water by siphoning from the bottom, changing half of the water body every four hours, and successfully hatch the fry within 36-38 hours.
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