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Artificial eel propagation method

A technology of artificial breeding and river eel, which is applied in fish farming, application, climate change adaptation, etc., can solve the problems of lack, low fertilization rate, and inability to meet mass production, so as to improve the level, reduce the dose of hormone injection, prevent Effects of anesthetics and procedural injuries

Inactive Publication Date: 2012-06-27
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of artificial reproduction, there are problems such as asynchronous male and female development, poor induction efficiency, low fertilization rate, unstable hatching, and open bait cannot be solved.
And the long-term lack of understanding of the ecological conditions for natural breeding in the ocean, the artificial reproduction of river eels has become a recognized problem in the world
In 2010, after more than 40 years of research, the Japanese Fisheries Research Center announced that it had completed the artificial reproduction of river eels, but the rate of induced birth, fertilization rate, hatching rate, and survival rate during the breeding process were extremely low, and high-cost input ( Seedlings are about 1 million yen per head), and the experimental operating conditions are far from meeting the needs of mass production
However, there is no report about the method of obtaining mature female and male eels under the control of artificial hormones, and obtaining a large number of fertilized eggs and early seedlings of river eels through artificial insemination and natural mating.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 broodstock ripening

[0023] Environmental factors: salinity 17-30‰, water temperature 16°C-24°C, pH 7.5-8.2, dissolved oxygen 4-15mg / L, stocking density 50 fish / pond, separate male and female.

[0024] The acclimatization period was carried out on the received parent river eel. Put the collected parent river eel into a cement pool (10m×3m×1.2m) with a half-shade cloth greenhouse, domesticate for 7 days to adapt to the artificial environment, introduce natural seawater (17‰) filtered by sedimentation, and use artificial sea salt Adjust to make the salinity of the pool water gradually rise to 20‰, and then every half month as a cycle, the salinity is increased by 3-4‰ until the salinity rises to 30-35‰. Add tiles, PVR tubes and other coverings under the sunshade net at the bottom of the pool. During the day, the fish hide in the covering, and at night they will move around in the area without the sunshade net, so as to avoid light during the day and see t...

Embodiment 2

[0026] Example 2 Broodstock induce labor

[0027] Environmental factors: salinity 30-35‰, water temperature 19.5-22.5°C, pH 7.0-8.0, dissolved oxygen 5-15mg / L, stocking density 12 fish / pond, male to female ratio 1:3.

[0028] After 2-3 weeks, the female eels that have begun to develop use their own reproductive regulation to reach the final water and action stage of ovary development. According to the state of the genital opening of the female eel (protruding and a little red and swollen), and the degree of change in abdominal circumference (appropriately enlarged but not inflated), the fish body is selected. The female eel is injected with the last injection of ripening agent in the morning of the first day, and injected with induced labor 24 hours later. The oxytocic agent is 17α-hydroxyprogesterone (OHP) (5mg / 500g·BW) mixed with HCG (150IU / 500g·BW). And select the male eels that can produce sperm, and inject a shot of CPE (0.5mg / 500g·BW) mixed with HCG (150IU / 500g·BW). ...

Embodiment 3

[0031] Embodiment 3 fertilized egg hatching

[0032]Environmental factors: strictly control salinity 33±0.5‰, water temperature 21±0.5, pH 7.2-7.5, dissolved oxygen above 6mg / L, ammonia and nitrogen content not exceeding 0.02mg / L and 0.05mg / L, Ca 2+ , Mg 2+ The mass concentration range is 325-330 mg / L, 1160-1165 mg / L.

[0033] The obtained fertilized eggs are immediately transferred to a special 50L circular cone-bottomed incubator for incubation. A small amount of aeration ensures sufficient dissolved oxygen, and the formation of a micro-water flow can drive the eggs to float. Hatching density: 600-800 grains / L. Change the water by siphoning from the bottom, changing half of the water body every four hours, and successfully hatch the fry within 36-38 hours.

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PUM

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Abstract

The invention relates to an artificial eel propagation method, which includes: a, ripening parent fishes, breeding catadromous parent reels for seven days, gradually increasing salinity of water, and subjecting the parent eels to back intramuscular injection of ripening agent-carp pituitary gland homogenate and HCG (human chorionic gonadotropin); b, performing induced spawning of the parent eels, subjecting female eels to injection of oxytocin 17 alpha-hydroxyl progesterone and HCG, subjecting sperm-producing male eels to injection of mixture of CPE (carp pituitary extract) with HCG, collecting naturally spawned fertilized eggs on the next day, and subjecting the female eels laying no eggs to semi-dry artificial insemination; and c, hatching the fertilized eggs, immediately transferring the fertilized eggs to a special-made hatching barrel for hatching, and slightly aerating, changing half of water every four hours until fry are hatched successfully. The artificial eel propagation method has the advantages that anesthetic loss and operational injury can be prevented, injection dosage of hormones is reduced, a great amount of eels can mate and lay eggs naturally under artificial induced spawning, artificial insemination can be complete securely in a planned way, level of high-quality fertilized eggs and alevin of eels artificially propagated is increased.

Description

technical field [0001] The invention relates to a method for artificial reproduction of river eels, specifically, river eels obtain sexually mature female eels and male eels under the control of artificial hormones, and obtain a large number of fertilized eggs and early seedlings of river eels through artificial insemination and natural mating Methods. Background technique [0002] River eel, scientific name Japanese eel ( Anguilla Japonica ), is one of the most important migratory fish in Asia. It has undergone several metamorphosis processes in its life history, growing and fattening in fresh water, and then spawning and reproducing in the ocean. At present, the seedlings needed for river eel farming are completely dependent on natural fishing. my country has experienced the process of exporting eel fry fishing in the 1970s, relying on imports for some eels in the early 1990s, and finding it difficult to catch seedlings in the Yangtze River Estuary in the late 1990s. Du...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘利平蒋天宝赵长春王磊陈文银吴嘉敏
Owner SHANGHAI OCEAN UNIV
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