In vitro maturation culture method of denuded oocytes of mice
An in vitro mature culture and naked egg technology, which is applied in the field of in vitro mature culture of mouse naked eggs, can solve the problems of the blastocyst stage that cannot be further developed to reach the uterine implantation, oocytes do not have the ability to combine with sperm and fertilize, and achieve The effect of promoting application and promotion, high efficiency of maturation and development, and high quality of embryonic development
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[0017] 1 Experimental method
[0018] 1.1 In vitro maturation and culture of naked eggs
[0019] Take the mouse ovary and use the head of a syringe needle under a microscope to puncture the follicle on the ovary, and release the oocytes in the follicle into the TCM199 oocyte in vitro operating solution containing HEPES and 10% FBS. TCM199 is a product of Therma , Product No. SH30233; After washing the naked eggs in vitro with an egg picking needle, randomly put them into oocyte maturation culture medium containing 50μM forskolin In In-Vitro MaturationMedia, naked eggs were cultured in oocyte maturation medium containing 50μM forskolin for 3h, then naked eggs were incubated in oocyte maturation medium containing 2.5μM PD166285 After washing in In-Vitro Maturation Media, transfer to oocyte maturation medium containing 2.5μM PD166285 In-Vitro Maturation Media continued in vitro culture for 16h. among them In-Vitro Maturation Media is a product of Sage in the United States, and the...
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