Micro fluidic chip for sorting and whole genome amplification of single cell

Abstract

The invention provides a micro fluidic chip for sorting and whole genome amplification of a single cell. The micro fluidic chip comprises a four-layer structure, wherein the four layers are stacked together in order and mutually sealed, and the structure comprises the following layers from top to bottom: a top cover, a micro valve control layer, a valve plate layer and a channel layer; and the bottom of the micro fluidic chip is externally provided with a magnet. The invention also provides a system for screening and identifying cells as well as amplification and analysis of a single cell, and the system is matched with the chip. The micro fluidic chip and the corresponding detection system can be used for sorting target cells from a large amount of cells quickly, simply and cheaply, and amplifying and analyzing whole genome DNA of a single cell therein.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a microfluidic chip used for single cell sorting and single cell whole genome amplification. Background technique [0002] A gene is a functional fragment carrying genetic information on a DNA molecule, and is a material for biological transmission of genetic information. The publication of the fine map of the human genome marks that the development of modern medicine has gradually entered the era of genomic medicine. Especially in the aspect of modern medicine, the important impact of genomic medicine on disease diagnosis, malignant tumor, organ transplantation, mental disease, cardiovascular disease, infectious disease, pharmaceutical, medical ethics and gene therapy has begun to appear. It is mainly reflected in the prediction of disease risk, disease prevention, scientific guidance of lifestyle and individual treatment of diseases. [0003] If cancer cells can be...

AI Technical Summary

Problems solved by technology

In the whole genome amplification technology based on PCR (polymerase chain reaction), during the PCR reaction, the difference in DNA fragments will affect the amplification efficiency of the polymerase and even cause the enzyme to slip or detach from the template, making it incomplete. Cover the genome accurately, and will introduce a lot of errors and non-specific amplification products into the sequence, there is amplification bias, when the initial sample is very rare, the amplification quality is greatly reduced, and only a single gene locus can be paired by specific primers at a time It is impossible to perform multi-site amplification and detection on the same sample, and it is impossible to analyze the whole gene of a single cell

However, the new amplification method based on MDA (Multiple Displacement Amplification) also has the phenomenon of large amplification bias and poor amplification quality under the existing method.

[0005] In the existing technology, there is no complete process integrating single cell capture, identification, whole gene amplification and analysis

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