The invention discloses a genetic marker group and an individual gene identification card and application thereof. The genetic marker group comprises multiple SNP sites, wherein the multiple SNP sitesare tagSNPs located in different haplotype areas, the MAF is between 0.48 to 0.505, the adjacent SNP sites are not in linkage relationships, the distance among the adjacent SNP sites is larger than 1M, and variation information non A / T or C / G and the SNPs are evenly distributed in all chromosomes. By applying the technical scheme, through effective combination of the SNP sites, the set of genetic marker group which is simple in technology, economical and practical is formed, and can be used as the individual DNA identification card, and when the SNP sites are selected, linkage relationships,the MAF, distance, variation situations and the evenness of distribution on the chromosomes of the sites are taken into account, so that the accuracy of judging a certain gene sample is improved.