Reconstructed oocyte of deaf model pig, construction method of reconstructed oocyte, deaf model pig, and construction method and application of deaf model pig

Abstract

The invention discloses a reconstructed oocyte of a deaf model pig and a construction method of the reconstructed oocyte, and also discloses the deaf model pig and a construction method and application of the deaf model pig. According to the reconstructed oocyte of the deaf model pig, the construction method of the reconstructed oocyte and the construction method of the deaf model pig, by knockingout a pig OSBPL2 gene, the reconstructed oocyte of a deaf mini-pig is obtained, and in combination with a CRISPR / Cas9 gene editing technology, a somatic cell nuclear transfer technology and an embryotransfer technology, high efficiency and feasibility of the construction method for the genetically engineered deaf model pig are proven. Through the construction method of the deaf model pig, genotypes and deafness phenotypes of human OSBPL2 gene mutations can be precisely duplicated, the pig of which an OSBPL2 gene is knocked out has typical characteristics of human autosomal dominant hereditary hearing loss (DFNA67), therefore, a reliable big animal model can be provided for disease research of the human hereditary hearing loss, and the deaf model pig can be applied to inner-ear gene repairing, inner-ear hair cell regeneration, hearing reconstruction and the like.

Description

technical field [0001] The invention belongs to the fields of molecular biology and genetic engineering, and in particular relates to a reconstructed egg of a deafness model pig and a construction method thereof, a deafness model pig, a construction method and an application thereof. Background technique [0002] Deafness is the most common sensory impairment disease. According to the latest statistics from the World Health Organization (WHO), there are about 466 million hearing disabled people in the world, of which nearly 34 million are children. The incidence of newborn deafness is 0.1-0.3%. 50% of deaf patients are related to genetic factors. According to whether it is accompanied by abnormalities, lesions or systemic diseases outside the ear, hereditary deafness is divided into syndromic hearing loss (SHL) and non-synthetic deafness (Non- syndromic HearingLoss, NSHL), NSHL accounts for about 70%. Autosomal dominant deafness (DFNA) and autosomal recessive deafness (DFNB...

AI Technical Summary

Problems solved by technology

Although the hearing structure and function of mice and other rodents and the phenotype of the mutants are similar to those of humans, they are quite different from humans in terms of developmental genetics, and cannot completely replicate the deafness phenotype of humans.

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