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Method for producing sex controllable in vitro embryo of buffalo

A technology of buffaloes and embryos, applied in the field of bioengineering, can solve problems such as not being able to meet the needs of the market, and achieve the effects of saving time, increasing quantity, and improving quality

Inactive Publication Date: 2005-08-17
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] Buffalo is the buffalo milk in southern China with high nutritional value, but judging from the current situation of milk buffalo in my country, its quantity and milk output are far from meeting the demand of the market

Method used

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specific Embodiment

[0019] Buffalo ovaries were collected from the slaughterhouse, placed in saline at 25°C, and brought back to the laboratory in a thermos bottle. Wash the ovaries with sterile normal saline for 3-4 times, and extract oocytes from follicles with a diameter of 2-8 mm on the surface of the ovary with a syringe equipped with No. 8. Under a stereo microscope, select oocytes with more than three layers of dense granule cells, bright appearance and uniform cytoplasm, buffered with Hepes and added with 5mmol / L NaHCO 3 (sodium hydroxide,) 3% OCS (estrus bovine serum) TCM199 oocyte collection solution was washed twice, then washed once with maturation solution, transferred to a petri dish for maturation culture. The maturation medium is Hepes-buffered TCM199+5% OCS+0.1 μg / mL FSH (follicle-stimulating hormone)+3% BFF (bovine follicle fluid). The maturation solution of each petri dish is 1mL, put 50-100 oocytes, 39°C, 5% CO 2 Cultivate for 21 to 24 hours under conditions of (carbon dioxi...

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Abstract

The method of producing extracorporeal buffalo embryo in controlled sex includes recovering buffalo egg in butchery or from living buffalo body, extracorporeal culturing to mature, external fertilization with fresh or frozen X or Y sperm of fine breed bull in a flow cytometer; and recovering embryo of the fertilized egg via external culture to blastula stage for transplanting or further freeze maintaining. The embryo is used in generating posterity of the expected sex. The present invention has the advantages of industrial embryo production in controlled sex and raised propagation speed of high yield milk buffalo.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for sex control of animals. Background technique [0002] The sex of mammalian individuals is determined by different chromosome reassignments and combinations during fertilization of sperm and eggs. The genetic basis of sex depends on the phenotype of sperm cells. If the sperm carrying the X chromosome combines with the egg, the chromosome combination of the fertilized egg is XX and develops into a female; if the sperm carrying the Y chromosome combines with the egg, the chromosome combination of the fertilized egg is XX. If it is XY, it develops into a male. Using separated X or Y sperm to artificially inseminate the dam or fertilize the oocyte in vitro, the sex of the offspring can be predicted before fertilization, so as to achieve the purpose of sex control. [0003] The use of sperm separation technology combined with in vitro fertiliza...

Claims

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Application Information

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IPC IPC(8): C12N5/06
Inventor 卢克焕张明陆阳清
Owner GUANGXI UNIV
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