177 results about "Cover slip" patented technology

Apparatus and methods for automatically staining or treating multiple tissue samples mounted on slides are provided, in which the slides and reagent bottles are held in fixed position, and the reagent, wash and coverslipping solutions brought to the slides. Alternatively, the slides are held in fixed position, while the reagent, wash and coverslipping solutions brought to the slides.

The invention discloses a cell sorter micro-fluidic chip based on the immunomagnetic separation technology and application thereof in the aspect of enrichment of rare cells, belonging to the technicalfield of micro-fluidic chips. The micro-fluidic chip, improved on the basis of the existing Y-type micro-fluidic chip, comprises a substrate and a cover-slip, wherein a Y-shaped micro-channel, a sample cell (1) and a buffer cell (2) respectively located at two end points of the upper part of the Y-shaped micro-channel, and an effluent cell (3) located at the end point of the lower part of the Y-shaped micro-channel are arranged on the substrate; and an antigen / antibody immunological reaction chamber (4) located on the micro-channel between the cross point (5) of the Y-shaped micro-channel andthe effluent cell (3) is further arranged on the substrate, wherein the width of the antigen / antibody immunological reaction chamber (4) is 2 to 5 times that of the micro-channel. The chip of the invention can effectively and rapidly fix the magnetic beads, increase the sample size of cells, improve the analysis flux, reduce the dead volume of sample introduction and reduce the retention and cross infection.

A cover slip mixing apparatus having a support and a flexible cover slip positioned over and forming a chamber between the support and the cover slip. A device is positioned with respect to the support and cover slip for applying a force on the cover slip and flexing the cover slip toward the support, the flexing cover slip providing a mixing action of a material located in the chamber. A microfluidic device includes a substrate with a fluid path disposed in the substrate. A flexible cover is positioned over the substrate and the fluid path, and a device is positioned with respect to the substrate and the cover. The device is operable to apply forces to the cover and flex the cover to act on fluid in the fluid path.

A cover slip material and a method of making and using the same are provided. The material comprises (a) a light transmissible polymeric backing having first and second surfaces; (b) a tack free bonding layer disposed on the first surface of the backing, the bonding layer comprising polymers selected from the group consisting of acrylate, methacrylate, and combinations thereof; and (c) a protective coating disposed on the second surface of the backing. Upon exposure to an environmentally friendly activating solvent such as terpene, d-limonene, esters of coconut oil and aliphatic hydrocarbon blends, the bonding layer acquires tacky properties causing the cover slip to adhere to a specimen previously mounted on a microscope slide.

A method of identifying the elements of a blood sample including placing an aliquot of blood on a transparent substrate such as a coverslip. The blood is allowed to stand and the cells to settle to form a layer or matrix. Inverted microscopy is used to identify the elements in the sample. Various forms of illumination may be used alone or in multiple combinations. The method improves the accuracy due to homogenous distribution of formed elements in the wet drop or aliquot, simplifies the method, lowers the cost of the test and results in a shortened analytical cycle time.

An open top microfluidic device comprising a microfluidic slide carrier and one or more multiplexing stations is provided which allows sequential staining and imaging without the need for using or removing a coverslip on a mounted biological sample.

The invention relates to a fiber array coupled with a VSCEL or PIN array and a manufacturing method of the fiber array. The fiber array comprises a cover slip, a carving groove substrate and a fiber micro-strip, the fiber micro-strip comprises an exposed fiber micro-strip portion without a fiber coating, a V-shaped carving groove for holding the exposed fiber micro-strip portion is formed in the carving groove substrate, the cover slip presses the groove of the exposed fiber micro-strip portion, the carving groove substrate, the cover slip and the exposed fiber micro-strip portion are fixedly connected through binding agents, the cover slip is shorter than the V-shaped carving groove, the end face of the exposed fiber micro-strip portion is an optical plane with a 45-degree oblique angle, and the optical plane faces the carving groove substrate. The coupling distance between the 45-degree optical plane of the exposed fiber micro-strip portion and the VSCEL or PIN array is controlled by the aid of the cover slip of the fiber array, the thickness of the cover slip can be customized, and high-efficiency coupling is realized. The method for manufacturing the fiber array is simple, easy to implement and low in cost.

A centrifugal device and method are provided for the separation of buoyant material such as parasitic ova from fecal matter. A rotor assembly, rotatable about its central axis in a centrifuge, includes a housing with a centrally located top opening leading to a centrally located mixing chamber. An annular sediment chamber is provided, also coaxial about the central axis, connected by a passage with the mixing chamber. A coring assembly is used to retrieve and insert a fecal sample into the mixing chamber for mixing with a flotation fluid. During centrifugation, heavier fecal components pass radially outwardly to the sediment chamber while the ova collect on the inward surface of the flotation fluid. After centrifugation, more flotation fluid is added, if needed, until a meniscus forms at the top opening. A coverslip is placed over the top opening and the ova float to the surface of the fluid and adhere to the coverslip. The coverslip is removed and the ova detected using standard microscopy procedures. In another aspect, a centrifugal device is provided in which the ova are delivered through centrifugation to a pipette tip for dispensing onto a microscope slide or coverslip.

The present approach relates to the measure of the planar tilt of a slide on a microscope using an integrated auto-focuser. The tilt of the slide can be used to detect improperly loaded slides (i.e. if the slide is resting on either the cover-slip or a printed barcode), compensate for misalignment between the microscope optical axis and the slide, and reduce subsequent focusing times.

The invention discloses a device and method for analyzing and counting blood cells by lensless holographic diffraction imaging, relating to blood cell detection and counting technology. The invention has a lensless simple structure and wide view, can realize miniaturization, and can monitor thousands of cells with different concentrations in real time. The device comprises a glass slide for placing a sample and a cover slip arranged on the glass slide, and is characterized in that a CCD or CMOS chip is arranged below the glass slide, a light source is arranged above the glass slide, an aperture is arranged between the light source and the glass slide and is arranged on the light extraction route of the light source. The method comprises the following steps: putting a blood cell sample between the glass slide and the cover slip and with well pressing, adjusting the light source above the glass slide, and adjusting the position of the sensitive chip below the glass slide to let the light emitted from the light source pass through the aperture and be vertically incident on the cover slip, and letting the CCD or CMOS ship arranged below the glass slide record the interference images after the light passes through the blood cell sample.

A controller for microscope lenses for correcting spherical aberration and for adjusting particularly difficult to access microscope lenses to an optimum of imaging quality, enabling automatic adjustment of the different actuators of a microscope lens, to effect a simple, cost-effective, user-friendly, and precise balance, particularly of cover slip deviations and different base thicknesses of Petri dishes for the purposes of increasing imaging quality. At least two movable elements of a microscope objective supporting lenses or lens groups, are provided in a movable manner in the axial direction along the optical axis of the microscope objective relative to the housing of the microscope lens in a motor-actuated manner by way of respective adjusting rings. A controller externally controlled and disposed in the microscope lens is provided for storing different characteristic curves for paths of motion of the movable elements.

The invention relates to a preparation method of a pitch microsection, which is a technology for preparing thin sections for microscopic analysis, and is used for solving the problems that the traditional thin section preparation method is troublesome and high in cost. In the method, the characteristics that the heated molten coal pitch has certain cohesive property, thus the volatilization loss does not exist and an internal structure is not damaged when the coal pitch is cooled and then cured are utilized to prepare the thin sections. The preparation method comprises the following specific steps: crushing pitch into particles; putting the pitch particles on a glass slide; heating the pitch particles for dehydrating; after the dehydration, rising the heating temperature so that an analytic sample is softened and melted; putting a coverslip on the analytic sample, and placing a leveling weight above the coverslip to pressurize the analytic sample, so that the analytic sample is in a thin section shape; rising the heating temperature, and stopping after carrying out heat preservation for a time; and naturally cooling the bubble-free analytic sample to obtain the thin section. he preparation method of the thin section, complex steps such as rubber mixing, slicing, grinding, polishing and the like are omitted, and the prepared polished section has the advantages of good planeness, low cost and high efficiency.

The invention discloses a medium accumulation conveyance device, wherein an impeller (5) can be avoided via a space-saving structure so as to prolong the service life of the impeller (5). The medium accumulation conveyance device flaps bank notes (14) via a rotating impeller (5) for the accumulation and sends out the accumulated bank notes (14). The impeller (5) comprises a plurality of flexible blades. The medium accumulation conveyance device comprises a cover slip holder (38) and an impeller cover (18) capable of moving in a direction vertical to the medium conveying direction is arranged on the cover slip holder (38). When the medium is accumulated, the cover slip holder (38) is moved, so that the impeller cover (18) deviates from the position of the impeller (5). When the medium is sent out, the cover slip holder (38) is also moved, so that the impeller cover (18) covers the impeller (5).

The present disclosure is directed to an apparatus and method for studying dissolution of a compact sample. The compact sample is typically a pharmaceutical drug sample. A flow-through apparatus includes a frame defining a flow-through channel, a removable insert having a drug sample, the insert positioned within the frame such that a fluid interacts with the sample when the fluid passes through the flow channel. The frame has an opening on the top side to allow a glass plate, typically a microscope cover slip to be positioned within the frame and allow viewing of the fluid flow and interaction with the drug sample. The hydrodynamics of the fluid flow are either known or computed. Thus, dissolution can be studied and observed in view of hydrodynamic characteristics. Typically, only a small amount of sample is necessary for a study. The flow-through apparatus is designed to fit on a microscopy stage and allow visual observation of the fluid / sample interaction.

A rear-end bearing accommodating portion that has a floored cylindrical shape is formed integrally on a rear bracket. A brush apparatus is mounted to the rear bracket by mounting a slinger portion onto an end portion of a shaft so as to cover slip rings. A rectifier is mounted to the rear bracket in close proximity to the rear-end bearing accommodating portion and the slinger portion. A rolling bearing is configured into a creep preventing bearing in which a resin band is mounted into a ring-shaped recessed groove that is recessed into an outer circumferential surface of an outer ring. A first slit is disposed through the slinger portion so as to be positioned so as to face downward when mounted to a vehicle and so as to communicate between an internal portion and an external portion of the slinger portion.

The invention relates to a cell opto-acoustic microscopic imaging method, which comprises the following steps: placing a cell on the concave surface of microscopic glass, irradiating the cell with laser light permeating the microscopic glass, producing opto-acoustic effect after the cell absorbs the light, transferring acoustic pressure variation produced by the opto-acoustic effect to an opto-acoustic sensor, and detecting the opto-acoustic variation and outputting an opto-acoustic signal by using the opto-acoustic sensor to realize opto-acoustic detection of the single cell; and performing two-dimensional scanning on the cell by using light beam scanning and a micro objective with high resolution to realize the opto-acoustic microscopic imaging of the single cell. The invention also relates to a cell opto-acoustic microscopic imaging device, which comprises a laser light scanning imaging mechanism, the opto-acoustic sensor and a signal processor. In the method and the device, light beam scanning technology without mechanical noise is combined with the micro objective with the high resolution to generate the opto-acoustic signal with high spatial resolution and then the opto-acoustic sensor is adopted for the opto-acoustic detection to perform the opto-acoustic microscopic imaging on the cell, wherein the solution is less than 1 micron.

The invention discloses an observation and density measurement method for glandular hairs on Artemisia annua leaf surfaces, comprising the following steps: taking a eugonic Artemisia annua leaf, and feeding the leaf in a centrifuge tube or a microtiter plate; successively adding petroleum ether or normal hexane, absolute ethyl alcohol or carbinol and glacial acetic acid, evenly mixing, and incubating for 5-20min at room temperature; and taking out the leaf, placing the left on a glass slide, adding a drop of double distilled water, covering a cover slip slightly, compacting by a tablet clip, and observing on an optical microscope or calculating the number of the glandular hairs so as to calculate the density of the glandular hairs. The method is simple and convenient, has low requirement on equipment, greatly simplifies the preprocessing procedure of experimental materials, can complete the whole operation within half a hour, uses a little of reagent, is non-toxic, safe and environment-friendly, lowers the measurement cost and time substantially, and can be used for high pass analysis.

The invention relates to an improved periodic acid Schiff (PAS) rapid staining kit. The kit comprises: a staining liquid, shedding-proof films, and cover slips. The staining liquid is a mixture of active fuchsine liquid and active fast green liquid, which is diluted with 95% of ethanol and 0.5% of sodium binitrite solution. The kit is advantaged in that: fuchsine and fast green are firstly prepared into a rapid biological rapid staining liquid; the staining process is fast, wherein the staining process takes 10 seconds; the detection process is simple, wherein a result can be observed directly, and washing is not required; the application scope is wide, wherein the kit can be used for staining various clinic specimens such as scurf, mucous and vaginal secretion. Periodic acid is attached on object slides, such that packing materials of the product is saved, and the operation process is simplified. The kit is especially suitable for a large clinic specimen amount. Because periodic acid, which is a flammable and combustible product, is attached to the object slides, periodic acid turns from liquid into a dried status. Therefore, danger of periodic acid is greatly reduced, and a standardized operation is easy to achieve. Poor repeatability due to personal operation errors can be avoided.

Methods for treating biological samples on microscope slides are set forth. One aspect of the invention is the use of predried reagents in wells on trays onto which the slides are placed, especially the use of predried reagents which dissolve sequentially. Yet another aspect of the invention is the use of external controls placed directly on a microscope slide in conjunction with a biological sample to be assayed. The external controls can be conveniently placed on a membrane which can be affixed to the slide. A further aspect of the invention is a specially designed tray to allow whole chromosome painting of all chromosomes of a cell sample on a single slide. The invention is also drawn to a coverslip with concave wells which act as reaction chambers when placed against a slide and filled with buffer. Preferably a reagent is predried in the well. A further aspect of the invention is a method of reacting samples on slides by placing them into a reaction chamber together with a coverslip which has a predried reagent on it.

The invention discloses a space transcriptome sequencing technology device based on tissue and chip space position information. The device comprises a cover slip and a silicon-based oligonucleotide chip, wherein an anchoring probe is arranged on the silicon-based oligonucleotide chip; a gap between the cover slip and the silicon-based oligonucleotide chip is used for accommodating a tissue freezing section with the thickness of 10-50 um. Meanwhile, the invention further provides a space transcriptome sequencing technology method adopting the device. According to the device and the method, sufficient RNA (ribonucleic acid) samples can be effectively acquired from a small quantity of cells, uniform or overall characterization of a sequencing result is avoided, further, difference of different phenotypes of cell transcript information in tissue can be distinguished according to cell heterogeneity, and transcript information with low abundance is mined, so that that more accurate transcript information with high specificity is provided for fields of biology, medicine and agriculture.

A clearing agent and mounting solution for microscopy is disclosed comprising (a) trichloroethanol, (b) optionally, trichloroacetic acid, (c) optionally, glycerol and (d) optionally, water, where the refractive index of the solution is greater than or equal to about 1.3810. The solution can further comprise a C1-C6 alcohol, other acids, and / or a stain. The solution can also comprise derivatives and / or analogs of 2,2,2-trichloroethanol and / or trichloroacetic acid. Also disclosed is a method of preparing specimens for microscopy comprising (a) applying a specimen to a microscope slide or a cuvette, (b) applying a quantity of the above solution sufficient to mount the specimen, and (c) optionally applying a cover slip. The solution can be used effectively with stains or dyes, and with fresh, partially dry or dried materials, and for temporary or semi-permanent to permanent mounting. The solution can be used with specimens or tissues / cells / parts originating from animals, poultry, livestock, humans, higher plants, yeasts, molds, microorganisms, insects, mites, or reptiles.

The present invention relates to a system (10) for handling slides, including a first and at least a second module-receiving area (24 through 28) for receiving at least one module (30, 34) for handling slides each. The first module-receiving area (24) accommodates a coverslipper module (30, 32) for coverslipping thin sections on slides with a mounting medium and a cover slip (48).

A plastic culture insert with a concavity replaces glass coverslip for supporting organic culture. It can be freely placed into and removed from a multi-well plate. The concavity anchors a tissue via collagen polymerization. The concavity is then, after organic culture, sealed by a regular slide for data storage and microscopy imaging analysis.

A rear-end bearing accommodating portion that has a floored cylindrical shape is formed integrally on a rear bracket. A brush apparatus is mounted to the rear bracket by mounting a slinger portion onto an end portion of a shaft so as to cover slip rings. A rectifier is mounted to the rear bracket in close proximity to the rear-end bearing accommodating portion and the slinger portion. A rolling bearing is configured into a creep preventing bearing in which a resin band is mounted into a ring-shaped recessed groove that is recessed into an outer circumferential surface of an outer ring. A first slit is disposed through the slinger portion so as to be positioned so as to face downward when mounted to a vehicle and so as to communicate between an internal portion and an external portion of the slinger portion.

The invention belongs to the technical field of fuel cells, and particularly relates to a micro membrane-free fuel cell with a three-dimensional porous carbon electrode and a preparation method thereof. The surface of a metal electrode lead is loaded with a carbon nanotube with a certain-thickness or carbon fiber to form the three-dimensional porous carbon electrode, and then is bonded and packaged by a cover slip to prepare the micro membrane-free fuel cell. The fuel and oxidant of the fuel cell respectively penetrate the three-dimensional porous carbon electrode loaded with a catalyst in a penetrative mode to conduct oxidation and reduction reaction, and a reactant (fuel and oxidant) liquid flow has high-efficient diffusion / convection substance transmission characteristics during penetrating the three-dimensional electrode. Reactants near an electrode consumption boundary layer can obtain sustained and effective supplement, so that the reactant concentration at the electrode can maintain a certain value. Meanwhile, as the carbon nanotube or the carbon fiber are loose and porous and have large specific surface area and more reactivity areas, the micro fuel cell has high fuel availability and a high power density.

The invention relates to a method of processing a powder sample for argon ion beam cutting. The method comprises steps: resin and a hardener are mixed to obtain a first mixture, the first mixture is mixed with the powder sample to obtain a second mixture, and the second mixture is placed on a slide and put in a vacuum drier for vacuum pumping; a cover slip is taken to sweep the second mixture on the slide on the cover slip, the second mixture is laid in a semicircle shape at the edge at one side of the cover slip, the cover slip is then put on a temperature control heating table for being cured, and a cover slip with first cured resin is obtained; the cover slip with the first cured resin is fully coated with the first mixture, the coating thickness is flush with the highest point of the first cured resin on the cover slip, the cover slip is buckled on a silicon wafer upside down and cured on the temperature control heating table, the first mixture fully coating the cover slip is cured into second resin, and an embedded sample is obtained; and the end surface of the embedded sample is polished and smoothed, the embedded powder sample is exposed, and the powder sample can then be put in an ion beam cutter for cutting.

A crystallization device for in situ x-ray diffraction of biological samples includes a flat plate made of single crystal quartz, silicon or sapphire and having an array of wells or lanes defined on the plate. The crystallization device may be in the form of a cover slide used with a crystallization plate in a vapor diffusion setup. Also disclosed is a method for preparing biological samples for crystallization and x-ray diffraction, including: obtaining a crystallization device made of single crystal quartz, silicon or sapphire; loading a solution containing biological molecules onto the crystal support device; maintaining the solution in a crystal growth environment to grow crystals of the biological molecules; removing the crystal support device carrying the crystals from the crystal growth environment; exposing the crystals carried on the crystal support device to an X-ray beam; and recording X-ray diffracted by the biological crystals.

The invention belongs to the technical field of fuel cells, and particularly relates to a micro membrane-free fuel cell with carbon-nanotube three-dimensional electrodes and a preparation method thereof. A metal electrode lead is arranged on the surface of a substrate material of the micro membrane-free fuel cell, and a plurality of carbon-nanotube three-dimensional electrodes and a plurality of carbon-nanotube diversion screen plates are grown on the metal electrode lead in a positioned mode. Catalysts are loaded on the carbon-nanotube three-dimensional electrodes. The substrate material and a cover slip with a groove are bonded and packaged to obtain the micro membrane-free fuel cell. The micro membrane-free fuel cell utilizes the carbon-nanotube three-dimensional electrodes loaded with the catalysts so as to increase the contact area of a working substance and the electrodes, the perturbation action of the three-dimensional electrodes on laminar substances strengthens the local diffusion of substances, and reactants near an electrode consumption boundary layer can obtain sustained and effective supplement, so that the cell has a high power density.

The invention belongs to the technical field of sensors, and particularly relates to a packaged light micro flow micro cavity biochemical sensor with a radial higher order mode kept, which comprises a section of tapered fiber, a light micro flow micro bubble cavity, a cover slip, an ultraviolet adhesive for packaging and fixing and a low-refractive index polymer, wherein the main component of the light micro flow micro cavity is silicon dioxide, the diameter is 100-500 micrometer and the wall thickness is 2-20 micrometer; the main component of the tapered fiber is silicon dioxide, the diameter is 2-5 micrometer, and the tapered fiber is used for exciting an echo wall mode in the micro cavity; and the low-refractive index polymer has a refractive index of 1-1.4 for fixing two ends of the tapered fiber and preventing light leakage. The device carries out sensing through monitoring the displacement condition of an optical mode resonance wavelength, and has the advantages of compact structure, small size, strong anti-interference performance, good stability, simple manufacturing process and low cost and the like. Besides, the device can keep the radial higher order mode in a coupled micro cavity, extremely low detection limit and extremely high sensitivity are realized, and the application prospect in the biochemical sensing field is good.