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Improved periodic acid Schiff reaction rapid staining kit

A periodic acid Schiff's, rapid dyeing technology, applied in biological testing, material inspection products, etc., can solve the problems of difficult to distinguish bacteria from background, difficult to control temperature, complicated operation, etc., to solve the problem of unsuitable for conventional long-distance storage and transportation. , to avoid the effect of poor repeatability and simplify the operation process

Inactive Publication Date: 2011-11-16
北京世济合力生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hexamine silver staining can display most fungi, and can display fungal characteristics very well. It is a method with the best effect to display fungi, but this method is cumbersome to operate, has more technical requirements, is not easy to control temperature, and the effect is unstable. , It is not easy to obtain the staining effect of bacteria and clear background, etc.
However, in clinical laboratory work, these two methods inevitably have disadvantages such as the need for special and dangerous chemical reagents, cumbersome operations, many technical points that are difficult to master, unstable effects, and difficult to distinguish between bacteria and backgrounds.

Method used

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  • Improved periodic acid Schiff reaction rapid staining kit

Examples

Experimental program
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Effect test

preparation example Construction

[0036] 1. Preparation of staining solution:

[0037] 1-1 Preparation of active fuchsin solution:

[0038] 1). Add 4-4.5g of basic fuchsin to 1 liter of deionized water, heat in a water bath at 95-100°C for 10-15min, and when cooled to 48-50°C, filter with filter paper with a pore size of 80-120 microns ; 2). Add HCL (1Mol / L) 90-100ml to the above-mentioned filtrate, adjust the pH value to <5, and cool to room temperature;

[0039] 3). Then add 9.5-10.5 g of sodium metabisulfite, stir for 5-10 minutes, put in a dark place overnight, add activated carbon powder, stir and filter to obtain a colorless and transparent dye solution, and store it in the dark at room temperature for later use.

[0040] 1-2 Preparation of Fast Green Working Solution:

[0041] 1). Take 1.8-2.2 g of solid chlorogen powder and add it to 10 ml of distilled water, stir for 30 minutes until completely dissolved, and obtain liquid A with a concentration of 180-220 g / L for later use.

[0042] 2). Take 0.5-0...

Embodiment 1

[0055] Embodiment 1: tinea pedis patient The experimental diagnosis process is as follows:

[0056] 1). Materials: 3 pieces of small dandruff from different parts between the toes were collected from a patient with tinea pedis in the Dermatology Department of Peking University Third Hospital;

[0057] 2). Apply these 3 pieces of small skin flakes on the anti-peeling film respectively;

[0058] 3). Add reactive fuchsin with a concentration of 4.3g / L and fast green working solution with a concentration of 1.6g / L, and mix it with 95% ethanol and 0.5% sodium bisulfite at a volume ratio of 2:1:4:2 The prepared staining solution is added to the cover glass until it completely infiltrates the specimen, and observed under a microscope after 10 seconds;

[0059] 4). The dyeing result is as follows: figure 1 and figure 2 As shown, there are a large number of hyphae and spores, combined with the analysis of mycelium and spore morphology, it is speculated that the patient is tinea ped...

Embodiment 2

[0061] Embodiment 2: tinea capitis patient

[0062] The experimental diagnosis process is as follows:

[0063] 1). Materials: Collected from patients with tinea capitis in the Department of Dermatology, Peking University Third Hospital, 3 pieces of small dandruff on different parts of the head;

[0064] 2). Apply these 3 pieces of small skin flakes on the anti-peeling film respectively;

[0065] 3). Add reactive fuchsin with a concentration of 4.3g / L and fast green working solution with a concentration of 1.6g / L, and mix it with 95% ethanol and 0.5% sodium bisulfite at a volume ratio of 2:1:4:2 The prepared staining solution is added to the cover glass until it completely infiltrates the specimen, and observed under a microscope after 10 seconds;

[0066] 4). The staining results are shown in Table 3 and Table 4. There are a large number of spores. Combined with the analysis of spore morphology, it is speculated that the patient is tinea capitis caused by the infection of th...

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Abstract

The invention relates to an improved periodic acid Schiff (PAS) rapid staining kit. The kit comprises: a staining liquid, shedding-proof films, and cover slips. The staining liquid is a mixture of active fuchsine liquid and active fast green liquid, which is diluted with 95% of ethanol and 0.5% of sodium binitrite solution. The kit is advantaged in that: fuchsine and fast green are firstly prepared into a rapid biological rapid staining liquid; the staining process is fast, wherein the staining process takes 10 seconds; the detection process is simple, wherein a result can be observed directly, and washing is not required; the application scope is wide, wherein the kit can be used for staining various clinic specimens such as scurf, mucous and vaginal secretion. Periodic acid is attached on object slides, such that packing materials of the product is saved, and the operation process is simplified. The kit is especially suitable for a large clinic specimen amount. Because periodic acid, which is a flammable and combustible product, is attached to the object slides, periodic acid turns from liquid into a dried status. Therefore, danger of periodic acid is greatly reduced, and a standardized operation is easy to achieve. Poor repeatability due to personal operation errors can be avoided.

Description

technical field [0001] The invention relates to a rapid staining kit for periodic acid Schiff reaction (PAS) method, in particular to an improved rapid staining kit for periodic acid Schiff reaction (PAS) method Background technique [0002] Fungi refer to microfibrils that have true nuclei, produce spores, do not contain chlorophyll, absorb nutrients in a parasitic or saprophytic manner, can reproduce sexually and / or asexually, and have cellulose (or other glucans) or chitin Or an organism with a cell wall that has both. Fungal invasion of the human body is often secondary to other diseases, such as chronic wasting diseases such as malignant tumors, TB, and diabetes; large doses of X-ray irradiation; immunosuppressants, etc. lead to low immune function and resistance; patients who use a large number of adrenal cortex hormones, Its inflammatory response is mostly suppressed, and it is also prone to fungal infection; in addition, organ transplantation, catheter intubation, r...

Claims

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Application Information

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IPC IPC(8): G01N33/52
Inventor 张丹韩亚京王雷明付红伟
Owner 北京世济合力生物科技有限公司
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