The invention relates to a culture method for efficiently obtaining adipose mesenchymal stem cells, and solves the problem that the cell viability obtained in the prior art is low. The culture method comprises the following steps: separation of the adipose mesenchymal stem cells: washing D.Hanks with fat tissues, detecting to guarantee no pollution, adding a solution mixture of I-type collagenase and trypLETM digestive enzyme with several times of volume, and stopping enzymolysis after carrying out rotary vibration digestion for dozens of minutes at the temperature of 37 DEG C, centrifuging to remove a liquid supernatant, and filtering with screen cloth to obtain an adipose mesenchymal stem cell suspension; culture of the adipose mesenchymal stem cells: inoculating a culture bottle bloodless culture medium with the adipose mesenchymal stem cell suspension, carrying out primary cell culture, observing with an inverted phase contrast microscope in the culture period, and changing a fresh culture medium, when the cells are cultured to 80% to 90% fusion, extensively inoculating a new culture bottle with the cells for culture after digestion with trypLETM, and subculturing for several generations to obtain the purified mesenchymal stem cells. The culture method has the advantages that the digestion is more sufficient, the cell yield is higher, the mesenchymal stem cells are protected from being damaged, and the viability is high.