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Method for preparing xylem sample of haloxylon ammodendron for scanning electronic microscope

A scanning electron microscope and sample preparation technology, applied in scanning probe technology, instruments, etc., can solve the problems of structural deformation, deformation and fragility of sliced ​​cells, and achieve the effect of complete sample structure, strong image stereoscopic effect and clear tissue structure.

Inactive Publication Date: 2015-12-16
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the hard material of Haloxylon xylem, the specific gravity of wood is greater than 1; the wood contains a lot of crystals (probably silicide), and the parenchyma junction between two adjacent growth zones in wood is particularly fragile
When using the above traditional method to cut the hard xylem of Haloxylon, because the xylem structure is compact and contains a large number of crystals, the tissue structure of the sample is deformed by the extrusion of the blade when slicing, and the fine structure of the section cells is greatly damaged, and easily broken
The cell structure of the prepared slices is deformed and damaged, which ultimately affects the quality of the scanning electron microscope images of the sample

Method used

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  • Method for preparing xylem sample of haloxylon ammodendron for scanning electronic microscope
  • Method for preparing xylem sample of haloxylon ammodendron for scanning electronic microscope
  • Method for preparing xylem sample of haloxylon ammodendron for scanning electronic microscope

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: a kind of Haloxylon xylem scanning electron microscope sample preparation method is characterized in that comprising the following steps:

[0021] a. Sample preparation: Cut Haloxylon xylem into 1×1×1 blocks, dry naturally for 1 month and polish until the surface is smooth;

[0022] b. Sample dehydration: use alcohol for gradient dehydration 3 times, each dehydration time is 40min, and the alcohol concentration is 50%, 60%, and 70% in each dehydration;

[0023] c. Sample drying: use liquid CO 2 Drying by critical point drying method;

[0024] d. Sample corrosion: soak the sample in 98% concentrated sulfuric acid for 5 minutes;

[0025] e. Sample sticking table: paste carbon-coated conductive adhesive tape on the sample table, trim the dried sample into a flat section of 3mm×3mm, and paste it on the conductive adhesive tape;

[0026] f. Sample coating: Spray the bonded sample with gold for 200s at a current of 15mA with an ion sputtering coater, and even...

Embodiment 2

[0027] Embodiment 2: a kind of Haloxylon xylem scanning electron microscope sample preparation method is characterized in that comprising the following steps:

[0028] a. Sample preparation: Cut Haloxylon xylem into 2cm×2cm×3cm blocks, dry naturally for 1 month and polish until the surface is smooth;

[0029] b. Sample dehydration: use alcohol for gradient dehydration 10 times, each dehydration time is 20min, and the alcohol concentration during each dehydration is 55%, 57%, 65%, 67%, 75%, 80%, 85%, 90% %, 95%, 100%;

[0030] c. Sample drying: use liquid CO 2 Drying by critical point drying method;

[0031] d. Sample corrosion: soak the sample in 98% concentrated sulfuric acid for 15 minutes;

[0032] e. Sample sticking table: paste carbon-coated conductive adhesive tape on the sample table, trim the dried sample into a flat section of 8mm×8mm, and paste it on the conductive adhesive tape;

[0033] f. Sample coating: Spray the bonded sample with gold for 200s at a current ...

Embodiment 3

[0034] Embodiment 3: a kind of Haloxylon xylem scanning electron microscope sample preparation method is characterized in that comprising the following steps:

[0035] a. Sample preparation: Cut Haloxylon xylem into 1cm×1cm×2cm blocks, dry naturally for 1 month and polish until the surface is smooth;

[0036] b. Sample dehydration: use alcohol for gradient dehydration 6 times, each dehydration time is 20 minutes, and the alcohol concentration is 51%, 62%, 71%, 82%, 91%, 99% in each dehydration;

[0037] c. Sample drying: use liquid CO 2 Drying by critical point drying method;

[0038] d. Sample corrosion: soak the sample in 98% concentrated sulfuric acid for 8 minutes;

[0039] e. Sample sticking table: paste carbon-coated conductive adhesive tape on the sample table, trim the dried sample into a flat section of 4mm×4mm, and paste it on the conductive adhesive tape;

[0040] f. Sample coating: Spray the bonded sample with gold for 200s at a current of 15mA with an ion sputt...

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Abstract

The invention discloses a method for preparing a xylem sample of haloxylon ammodendron for a scanning electronic microscope. The method is characterized by mainly comprising the steps of sample preparation, sample dehydration, sample drying, sample corrosion, bonding the sample to a table and coating the sample with a film. Compared with the prior art, the sample is complete in structure, the organization structure in the tangent plane of the xylem is clear when the sample is observed by the scanning electronic microscope, images are highly stereoscopic, and the ultrastructure of the xylem of the haloxylon ammodendron can be conveniently observed and analyzed.

Description

technical field [0001] The invention belongs to the technical field of microscopic observation samples, and relates to a preparation method of scanning electron microscope samples, in particular to a preparation method of Haloxylon xylem scanning electron microscope samples. Background technique [0002] Haloxylon is a plant of the genus Haloxylon Chenopodiaceae, a shrub or a small semi-tree. It is the construction species of the Haloxylon desert vegetation, which is widely distributed in sub-desert and non-desert regions, and forms a unique horizontal landscape. Haloxylon has the characteristics of super-xerophytic, salt-tolerant, and wind-erosion-resistant. It is used as a windbreak and sand-fixing afforestation tree species in many areas, and plays an important role in desert vegetation restoration. Therefore, it is particularly important to carry out research on the adaptability of Haloxylon morphological and physiological characteristics to desert environments for under...

Claims

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Application Information

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IPC IPC(8): G01Q30/20
Inventor 宋于洋周朝彬
Owner SHIHEZI UNIVERSITY
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