Preparation method and application thereof for cell-biological bracket compound based on biological print technology

Abstract

The invention provides a preparation method and the application thereof for a cell-biological bracket compound based on biological print technology. The cell-biological compound is formed by fibrous protein which is drawn out from the autoblood of a patient and processed by an ink-jet print technology. The surface and the interior of the cell-biological compound encompass one or more trophic factors and bone mesenchymal stem cells derived from self. According to the invention, the biological print technology is adopted, the appearance shape, cells and encompassing state model of the trophic factors can be designed according to requirements and practical situation, the cell-biological bracket compound is obtained through printing accurately, the fibrous protein and the bone mesenchymal stem cells are derived from self, so that the problem of immunological rejection is avoided, the trophic factors are cultivated in the bracket and are released slowly with the degradation of the bracket material, the cell-biological compound is applied to the spinal cord injured part to promote nerve regeneration and functional reconstruction on the injured part.

Description

technical field [0001] The invention relates to the construction of a cell-bio-scaffold compound for treating spinal cord injury, in particular to a preparation method and application of the cell-bio-scaffold compound based on bioprinting technology. Background technique [0002] Spinal cord injury is a serious traumatic disease of the central nervous system, which has the characteristics of high consumption and high disability rate, and brings huge damage and heavy burden to the society. Difficulty in nerve regeneration after spinal cord injury due to interruption of spinal cord continuity, neuronal loss, lack of neurotrophic factors, glial scarring, and cavitation. Traditional treatment methods such as drugs and surgery cannot solve the problem of regeneration, while the source of allogeneic acellular scaffolds is limited and there is a large immune rejection. In recent years, basic research, especially the development of nerve tissue engineering technology, has made grea...

AI Technical Summary

Problems solved by technology

Although this method has achieved some great success in the construction of simple cavity tissues and organs, it has many shortcomings, especially in the construction of tissues and organs with complex internal structures. In this method, cells cannot be precisely planted into The inside of the scaffold is only randomly adsorbed on the surface. Cell activity factors can only affect the growth and differentiation of cells on the surface of the scaffold, but it is difficult to control the activity of cells inside the scaffold. In view of the limitations of traditional tissue engineering methods, looking for more scientific tissue engineering technology is imminent