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Tissue engineering nerve graft prepared by biological printing technology and preparation method thereof

A technology of tissue engineering and bioprinting, which is applied in the fields of medicine and biomedical engineering, can solve the problems of cumbersome steps and limited tensile strength of nerve conduits, and achieve the effects of abundant sources, avoiding immune tissue reaction, and safe and controllable production process

Active Publication Date: 2015-04-22
南通大学技术转移中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method requires multiple stainless steel needles of a certain diameter to be fixed in parallel in the chitosan tube, the steps are cumbersome, and the tensile strength of the nerve guide is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Prepare 50ml of benzoic acid solution of 3% polylactic acid (the concentration of benzoic acid solution is 0.1mol / L) and put it in the ink cartridge of model HP51626A;

[0050] (2) Adjust the printer nozzle needle diameter to 2μm, the number of needles to 4, the distance from the nozzle to the bottom layer to 10mm, and the booster pulse frequency to 1v;

[0051] (3) Accurately simulate the three-dimensional space structure of different nerves, compile corresponding control programs, preset the three-dimensional model on the printer, and design the shape of the tissue-engineered nerve graft: the diameter of the outer tube is 1 mm, the wall thickness is 0.5 mm, and the diameter of the nanofiber scaffold in the tube is 0.1mm. The number of fiber supports is 5, and the fiber supports are evenly distributed in the tube. Print with the parameters of step (2) to form the outer tube and the nanofiber scaffolds evenly distributed inside the tube.

[0052] Or, accurately si...

Embodiment 2

[0059] (1) Prepare 50ml of citric acid solution of 5% sodium alginate (the concentration of citric acid solution is 0.05mol / L) and put it in the ink cartridge of model HP51626A;

[0060] (2) Adjust the printer nozzle needle diameter to 100μm, the number of needles to 16, the distance from the nozzle to the bottom layer to 25mm, and the booster pulse frequency to 15v;

[0061] (3) Accurately simulate the three-dimensional space structure of different nerves, compile corresponding control programs, preset the three-dimensional model on the printer, and design the shape of the tissue-engineered nerve graft: the diameter of the outer tube is 1 mm, the wall thickness is 0.5 mm, and the diameter of the nanofiber scaffold in the tube is 0.1mm. The number of fiber supports is 5, and the fiber supports are evenly distributed in the tube. Print using the parameters of step (2) to form the outer tube and the nanofiber scaffold inside the tube.

[0062] Alternatively, accurately simulat...

Embodiment 3

[0069] (1) Prepare 50ml of acetic acid solution of 5% chitosan (the concentration of acetic acid solution is 0.1mol / L) and put it in the ink cartridge of model HP51626A;

[0070] (2) Adjust the printer nozzle needle diameter to 50μm, the number of needles to 9, the distance from the nozzle to the bottom layer to 40mm, and the booster pulse frequency to 10v;

[0071] (3) Accurately simulate the three-dimensional space structure of different nerves, compile corresponding control programs, preset the three-dimensional model on the printer, and design the shape of the tissue-engineered nerve graft: the diameter of the outer tube is 1 mm, the wall thickness is 0.5 mm, and the diameter of the nanofiber scaffold in the tube is 0.1mm. The number of fiber supports is 5, and the fiber supports are evenly distributed in the tube. Print using the parameters of step (2) to form the outer tube and the nanofiber scaffold inside the tube.

[0072] Or, accurately simulate the three-dimension...

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Abstract

The invention provides a tissue engineering nerve graft based on a biological printing technology and a preparation method thereof. The tissue engineering nerve graft comprises an outer tube and a tube inside scaffolds, wherein trophic factors and / or cells can cover the inner surface and the outer surface. A high-fidelity printing operation is performed according to the actual nerve shape demand by utilizing the biological printing technology, a polymer solution is printed into the specified nerve graft in a three-dimensional way by using an ink-jet printer by adjusting the sizes and the quantity of the jet nozzles of the ink-jet printer, the distances from the jet nozzles to a bottom layer and the pulse frequency of a supercharger and writing a control program of specific printing, and the specified nerve graft is applied to treatment of peripheral nerve defect and spinal cord injury.

Description

technical field [0001] The invention relates to the fields of medicine and biomedical engineering, in particular to a tissue engineered nerve graft based on bioprinting technology and a preparation method thereof. Background technique [0002] In the real world, traffic accidents, work accidents, sports accidents, earthquakes, wars, and clinical operations will all cause peripheral nerve damage. Clinically, long-distance nerve defects cannot be compensated for by end-to-end sutures. Don't rely on a graft to bridge the repair. At present, autologous nerve transplantation is still the best way to treat these diseases clinically. Due to the limited source of autologous nerves for transplantation, new trauma, difficulty in matching tissue structure and size, and long-term denervation of the donor site, the application of autologous nerve transplantation is very limited. In recent years, tissue engineering based on the principles and technologies of life science and engineering...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/02A61L27/18A61L27/20A61L27/22A61L27/24A61L27/38A61L27/54
Inventor 杨宇民顾晓松丁斐赵亚红
Owner 南通大学技术转移中心有限公司
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