The invention discloses a temporo-mandibular joint biological condyle constructed based on a tissue engineering related technology. The temporo-mandibular joint biological condyle is characterized bycomprising an upper cartilage layer, a middle cellular hydrogel layer and a lower phrenological compound layer which are mutually laminated, wherein the upper cartilage layer comprises a tissue surface making contact with an upper articular disc, a connecting surface making contact with the middle cellular hydrogel layer and a dense tissue layer positioned between the tissue surface and the connecting surface. According to the temporo-mandibular joint biological condyle disclosed by the invention, on the basis of ensuring accurate control over a three-dimensional morphological structure, functional reconstruction of a temporo-mandibular joint is realized; besides, the temporo-mandibular joint biological condyle has the advantages of reducing operative wound, facilitating the use in the operation, reducing osteotomy and bone grinding change, avoiding skull base damage and massive hemorrhage risk and the like so as to overcome the defects of current artificial joint replacement and costal cartilage grafting; transformation from joint reconstruction and replacement to joint reconstruction and regeneration is realized.