Preparation method of interpenetrating network composite hydrogel

Abstract

The invention relates to a preparation method of composite hydrogel with an interpenetrating network structure. The preparation method comprises the following process steps of: (1) preparing a collagen solution with acetic acid, and preparing methacrylated chondroitin sulfate solution and methacrylated hyaluronic acid solution with deionized water, distilled water or phosphate buffer solution; (2) adjusting the pH value of the collagen solution prepared in the step (1) to 7.0-7.4 with sodium hydroxide solution under stirring at normal pressure and 0-4 DEG C, then adding the methacrylated chondroitin sulfate solution and methacrylated hyaluronic acid solution prepared in the step (1) to the collagen solution under stirring, uniformly mixing three solutions, then adding phosphate buffer solution for diluting until the collagen concentration in the mixed solution is 5-9mg / mL, then adding an initiator and a catalyst, and uniformly mixing to form a precursor solution; and (3) injecting theprecursor solution prepared in the step (2) to a mold, and placing the mold holding the precursor solution in an environment of normal pressure and 37 DEG C for 30-60minutes.

Description

technical field [0001] The invention belongs to the field of tissue engineering materials, in particular to a preparation method of collagen / chondroitin sulfate / hyaluronic acid interpenetrating network composite hydrogel. Background technique [0002] Articular cartilage injury is one of the common intractable diseases in clinical practice. Articular cartilage is limited by its own physiological structure, and often cannot repair itself after injury. Although the current clinical methods for the treatment of articular cartilage damage have certain short-term curative effect, they are all based on fibrocartilage repair, lacking the mechanical properties and durability of normal hyaline cartilage, so the repair effect is usually poor, and it is difficult to obtain satisfactory long-term results. Phase clinical effect. [0003] Cartilage tissue engineering provides a new method for the treatment of cartilage damage. Cartilage tissue engineering is to embed or plant seed cell...

AI Technical Summary

Problems solved by technology

[0005] However, the collagen / chondroitin sulfate / hyaluronic acid (CCH) composite hydrogel obtained by various methods currently does not have a complex network structure similar to natural cartilage tissue, only collagen fiber network or CS and HA pass through. The cross-linked network formed by the cross-linking agent and collagen, so the obtained CCH composite hydrogel has improved mechanical properties and degradation properties, but still needs to be further improved

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