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Collagen-chitosan / fibrin glue asymmetric bracket and the preparing method and the application thereof

A technology of fibrin glue and fibrinogen, applied in medical science, prosthesis, etc., to achieve the effect of good cell compatibility

Inactive Publication Date: 2007-10-31
韩春茂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There is no related report on the composite of fibrin glue and collagen / chitosan porous scaffold to make porous scaffold material suitable for tissue engineering skin construction

Method used

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  • Collagen-chitosan / fibrin glue asymmetric bracket and the preparing method and the application thereof
  • Collagen-chitosan / fibrin glue asymmetric bracket and the preparing method and the application thereof
  • Collagen-chitosan / fibrin glue asymmetric bracket and the preparing method and the application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Histological observation of collagen-chitosan / fibrin glue asymmetric scaffold.

[0044] Collagen and chitosan were dissolved in 0.5mol / L acetic acid solution respectively, and a concentration of 0.5% collagen solution and a 0.5% chitosan solution were prepared, and the chitosan solution was added to the collagen solution, wherein chitosan The content of sugar (by weight) is 10%, and it is stirred evenly; it is frozen at -20° C. for 2 hours by a freeze-freeze-drying method, and then freeze-dried in a freeze dryer for 24 hours. The lyophilized collagen / chitosan scaffold was vacuum dry-heated and cross-linked in a vacuum oven at 105°C for 24 hours, and placed in 1-ethyl-3-3 (dimethylaminopropyl )-carbodiimide solution (40mmol / l) and N-hydroxysuccinimide mixed solution for cross-linking for 24 hours, after repeated rinsing with three distilled water, freeze-lyophilize again to obtain cross-linked collagen-chitosan Sugar porous scaffold. Place the collagen-chitosan porous ...

Embodiment 2

[0047] Ultrastructural observation of collagen-chitosan / fibrin glue asymmetric scaffold under scanning electron microscope.

[0048] Collagen and chitosan were dissolved in 0.5mol / L acetic acid solution respectively, and a concentration of 0.5% collagen solution and a 0.5% chitosan solution were prepared, and the chitosan solution was added to the collagen solution, wherein chitosan The content of sugar (by weight) is 10%, and it is stirred evenly; it is frozen at -20° C. for 2 hours by a freeze-freeze drying method, and then placed in a freeze dryer for 24 hours. The lyophilized collagen / chitosan scaffold was vacuum dry-heated and cross-linked in a vacuum oven at 105°C for 24 hours, and placed in 1-ethyl-3-3 (dimethylaminopropyl )-carbodiimide solution (40mmol / l) and N-hydroxysuccinimide mixed solution for cross-linking for 24 hours, after repeated rinsing with three distilled water, freeze-lyophilize again to obtain cross-linked collagen-chitosan Sugar porous scaffold. Pla...

Embodiment 3

[0051] Study on Cytocompatibility of Collagen-Chitosan / Fibrin Glue Asymmetric Scaffold

[0052] Collagen and chitosan were dissolved in 0.5mol / L acetic acid solution respectively, and a concentration of 0.5% collagen solution and a 0.5% chitosan solution were prepared, and the chitosan solution was added to the collagen solution, wherein chitosan The content of sugar (by weight) is 10%, and it is stirred evenly; it is frozen at -20° C. for 2 hours by a freeze-freeze-drying method, and then freeze-dried in a freeze dryer for 24 hours. The lyophilized collagen / chitosan scaffold was vacuum dry-heated and cross-linked in a vacuum oven at 105°C for 24 hours, and placed in 1-ethyl-3-3 (dimethylaminopropyl )-carbodiimide solution (40mmol / l) and N-hydroxysuccinimide mixed solution for cross-linking for 24 hours, after repeated rinsing with three distilled water, freeze-lyophilize again to obtain cross-linked collagen-chitosan Sugar porous scaffold.

[0053] Soak the collagen-chitosa...

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Abstract

The invention discloses a collagen-chitose or fibrin glue asymmetric support, preparing method and application, which is characterized by the following: incorporating collagen-chitose porous material layer and fibrin glue layer; arranging the fibrin glue layer on the surface of collagen-chitose porous material layer evenly; forming interface between the fibrin glue layer and the surface of collagen-chitose porous material layer. This product possesses good cell compatibility, which is fit for complexing skin construction. This invention also discloses the preparing method of the asymmetric support and application.

Description

technical field [0001] The invention relates to a porous support material suitable for tissue engineering skin construction and a preparation method thereof, in particular to a collagen-chitosan / fibrin glue asymmetric support and a preparation method and application thereof. Background technique [0002] Clinically, skin defects caused by various reasons such as burns, trauma, diabetes, and venous ulcers are common diseases. The current conventional treatment method is to do skin grafting, such as autologous skin grafting, allograft skin or xenograft skin grafting. Among them, autologous skin transplantation is regarded as the first choice of treatment because there is no problem of rejection. However, the problem of insufficient autologous skin sources often exists, especially when encountering a large area of ​​burn patients, this contradiction between supply and demand is more prominent. In the past 20 years, with the development of material sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60
Inventor 韩春茂孙锦章石海飞毛峥伟周杰马列高长有
Owner 韩春茂
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