Artificial blood vessel stent with aligned fibers and manufacturing method of artificial blood vessel stent

A technology of artificial blood vessels and fiber orientation, applied in fiber processing, non-woven fabrics, pharmaceutical formulations, etc., to achieve the effect of narrow fiber diameter distribution, simple equipment, and favorable diffusion

Inactive Publication Date: 2017-03-22
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of existing artificial vascular stents and inspired by natural blood vessels, the present invention proposes a method for electrospinning to prepare a vascular stent imitating the fiber layer of smooth muscle orientation in human blood vessels, and guides the orientation of smooth muscle through the orientation of the fibers arrangement, the present invention has successfully prepared an artificial vascular scaffold that guides the alignment of smooth muscle cells, and is expected to be applied in clinical use

Method used

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  • Artificial blood vessel stent with aligned fibers and manufacturing method of artificial blood vessel stent
  • Artificial blood vessel stent with aligned fibers and manufacturing method of artificial blood vessel stent

Examples

Experimental program
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Effect test

Embodiment 1

[0029] In the first step, the pure polylactic acid (PLA, (C 6 H 8 O 4 ) n ) Is dissolved in a mixed solvent (the mass ratio of acetone and N,N-dimethylformamide in the mixed solvent w / w=1:1), and is formulated into an 8wt% solution as a spinning solution.

[0030] The second step is to place the spinning solution in a syringe pump equipped with a needle with a diameter of 0.5mm, apply a voltage of 10kV and perform electrospinning at a working distance of 15cm, and receive the fiber through a rotating motor with a stainless steel tube. Adjust the rotation speed of the rotating electric machine to 2000r / min, and obtain the fiber membrane with orientation arrangement.

[0031] In the third step, the stainless steel tube with the fiber membrane is soaked in deionized water for 30 minutes to remove the tube, and then dried at 35°C to obtain the target artificial blood vessel stent. Such as figure 1 In the artificial blood vessel stent shown, the artificial blood vessel stent is composed...

Embodiment 2

[0033] Put pure polyurethane (PU, C 28 H 44 N 2 O 11 ), polycaprolactone (PCL, C 6 H 10 O 2 ) The mixture of the two is dissolved in a mixed solvent (the mass ratio of acetone and N,N-dimethylformamide in the mixed solvent w / w=1:1), and a 10wt% solution is prepared as a spinning solution;

[0034] Put the spinning solution in a syringe pump equipped with a needle with a diameter of 0.5~1.0mm, apply a voltage of 15kV and perform electrospinning at a working distance of 10cm, receive nanofibers through a rotating motor with a metal tube, and adjust the rotating motor The oriented fiber can be obtained on the outer circumference of the metal tube. The rotation speed of the rotating electric machine is 2400 r / min.

[0035] Then, the stainless steel tube with oriented fibers was soaked in deionized water for 1 hour to realize the tube removal, and dried at 30°C to obtain an artificial blood vessel stent with oriented fibers. The artificial blood vessel stent is composed of oriented nan...

Embodiment 3

[0037] The pure polycaprolactone (PCL, (C 6 H 10 O 2 ) n ), a mixture of polylactic acid-glycolic acid copolymer (PLGA, 75:25) dissolved in a mixed solvent, the mixed solvent is acetone and N, N-dimethylformamide mass percentage w / w=1 :4, formulated into a spinning solution with a mass percentage of 15wt%.

[0038] The spinning solution is placed in a syringe with an inner diameter of 1mm and fixed on a syringe pump. The oriented composite fiber membrane is obtained by electrospinning. The electrospinning parameters are voltage 25kV, receiving distance 15cm, and motor rotation speed 600r / min, to obtain an oriented and aligned artificial blood vessel fiber membrane.

[0039] Then it is soaked in deionized water for 30 minutes, taken off the tube, and then dried at 30°C to obtain the target artificial blood vessel stent. The fiber arrangement of the target artificial blood vessel stent is an oriented structure, and can guide smooth muscle cells to oriented arrangement, and has good...

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Abstract

The invention discloses an artificial blood vessel stent with aligned fibers and a manufacturing method of the artificial blood vessel stent, and belongs to the field of tissue engineering. According to the artificial blood vessel stent provided by the invention, a bio-compatible polymer material is taken as a spinning solution; firstly, the influence of different rotating speeds on fiber alignment is researched by virtue of an electrostatic spinning method, so that a rotating speed that the best alignment degree is achieved is determined; and then, the aligned fiber artificial blood vessel stent is manufactured on the basis of the rotating speed; the artificial blood vessel stent is relatively good in biocompatibility; and in addition, the alignment of smooth muscle cells on blood vessels can be guided. The artificial blood vessel stent provided by the invention is applicable to large-scale production and is expected to be applied to actual clinical diagnosis.

Description

Technical field [0001] The invention belongs to the technical field of tissue engineering, and specifically relates to an artificial blood vessel stent with fiber orientation arrangement and a preparation method thereof. Background technique [0002] In recent years, various vascular diseases have become the number one killer of human health. At present, the main method for treating vascular diseases is vascular transplantation. Autologous blood vessels are the best choice for vascular transplantation. Due to their limited sources, they cannot meet the increasing demand for artificial vascular stents. The ideal artificial blood vessel stent needs to meet the following conditions: firstly, it has good biocompatibility, non-toxic to cells, non-toxic to the human body and non-carcinogenic, and has good mechanical properties, matches with natural blood vessels, is convenient, fast and cheap. Biocompatibility is the most basic prerequisite for the use of vascular stents. Many scient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/50A61L27/56D04H1/728D01D5/00
CPCA61L27/18A61L27/507A61L27/56A61L2400/12D01D5/0069D01D5/0092D04H1/728C08L67/04C08L75/04
Inventor 赵勇王女刘凯
Owner BEIHANG UNIV
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