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Preparation and application of chitosan-based nano-fiber

A nanofiber, chitosan-based technology, applied in fiber processing, fiber chemical characteristics, spinning solution preparation, etc., can solve problems such as complex process, achieve simple process, improve cell compatibility, and have broad application prospects.

Inactive Publication Date: 2008-10-08
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in general, electrospinning technology requires special equipment and complex processes, and the diameter of the prepared nanofibers is about hundreds of nanometers, which is larger than the nanofibers of natural extracellular matrix.

Method used

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  • Preparation and application of chitosan-based nano-fiber
  • Preparation and application of chitosan-based nano-fiber
  • Preparation and application of chitosan-based nano-fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 Preparation of chitosan / sodium phosphate tripolyphosphate nanofibers

[0030] The preparation method of chitosan / sodium tripolyphosphate nanofiber comprises the steps:

[0031] (1) Take by weighing 0.003 gram of chitosan (degree of deacetylation 85%) and be dissolved in the adipic acid solution of 30 milliliters 2% (g / mL), preparation concentration is the chitosan acetonitrile of 0.01% (g / mL). Diacid solution;

[0032] (2) Weigh 0.001 gram of sodium tripolyphosphate and dissolve it in 10 milliliters of deionized water to make a sodium tripolyphosphate solution with a concentration of 0.01% (g / mL);

[0033] (3) Under magnetic stirring at normal temperature, the chitosan adipic acid solution of 0.01% (g / mL) is added in the sodium tripolyphosphate solution of 0.01% (g / mL) with the speed of 1mL / min, keeps and Control the pH of the total system of the mixed solution between 3 and 6 until the dropwise addition is completed, and then stir for 1 hour;

[0034] (...

Embodiment 2

[0035] Example 2 Preparation of chitosan / sodium alginate nanofibers

[0036] The preparation method of chitosan / sodium alginate nanofiber, its step is as follows:

[0037] (1) Take by weighing 0.03 gram of chitosan (deacetylation degree 91%) and be dissolved in the adipic acid solution of 30 milliliters 2% (g / mL), preparation concentration is the chitosan acetonitrile of 0.1% (g / mL). Diacid solution;

[0038](2) Weigh 0.01 gram of sodium alginate and dissolve it in 10 milliliters of deionized water to make a sodium alginate solution with a concentration of 0.1% (g / mL);

[0039] (3) Under magnetic stirring at room temperature, add 0.1% (g / mL) chitosan adipic acid solution to 0.1% (g / mL) sodium alginate solution at a speed of 1 mL / min, keep and control The pH of the total system of the mixed solution is between 3 and 5 until the dropwise addition is completed, and then stirred for 1 hour;

[0040] (4) Centrifuge the solution obtained in the above step (3) at 20,000rpm for 30m...

Embodiment 3

[0041] Example 3 Preparation of collagen / chitosan / sodium tripolyphosphate nanofibers

[0042] (1) Take by weighing 0.03 gram of chitosan (deacetylation degree 100%) and be dissolved in the adipic acid solution of 30 milliliters 2% (g / mL), the preparation concentration is the chitosan acetonitrile of 0.1% (g / mL). Diacid solution;

[0043] (2) Take by weighing 0.003 gram of collagen and dissolve it in chitosan solution to obtain a composite solution of chitosan / collagen; Take by weighing 0.01 gram of sodium tripolyphosphate and dissolve it in 10 milliliters of deionized water to form a concentration of 0.1% (g / mL) of sodium tripolyphosphate solution;

[0044] (3) Under magnetic stirring at normal temperature, the chitosan adipic acid solution of 0.1% (g / mL) is added in the sodium tripolyphosphate solution of 0.1% (g / mL) with the speed of 1mL / min, keeps and Control the pH of the total system of the mixed solution between 3 and 5 until the dropwise addition is completed, and th...

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Abstract

The invention relates to a preparation method and application of chitosan-based nano-fiber, in which the preparation method includes the following steps that the drugs or bio-activator such as growth factors are dissolved or dispersed in the chitisan adipic acid solution with the concentration of 0.01% to 3% (g / ml), and then the gelatin or collagen are added to dissolve. The biodegradable polyanion is dissolved in the de-ionized water and in preaparation to be the solution with the same concentration as that of the chitisan. The mixed chitisan solution is slowly added into the polyanion solution. After stirred uniformly, the nano-fiber loaded with the bio-activator is obtained through centrifuging, washing and cooling-drying. The chitosan-based nano-fiber prepared through the invention is biodegradable and has the imitated extracellular matrix structure, in which the controlled release of bio-activator can be achieved. By the adding of collagen or gelatin, the cellular compatibility of nano-fiber or the control of the release speed of the bio-activator can be improved. Therefore, the invention has a broad prospect in the fields of tissue engineering, clinical wound healing and so on.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method and application of chitosan-based nanofibers. Background technique [0002] Natural polymer chitosan has low toxicity, good biocompatibility and biodegradability, and is widely used in biomedical fields. On the one hand, the structure of chitosan is similar to that of glycosaminoglycans in extracellular matrix (ECMs), which can be prepared into macroscopic shapes with different microscopic topography, and has a certain mechanical strength to adapt to the repair of tissue defects in different parts The required three-dimensional porous scaffold can be used as a three-dimensional carrier for cells in the field of tissue engineering to repair or reconstruct diseased tissues; Slow-controlled release system for biologically active substances. Especially in the fields of tissue engineering, wound repair and release of drugs / bioactive substances, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01D1/02D01D5/00D01D5/40D01F9/00D01F9/08D01F9/04D01F8/02D01F8/18
Inventor 曾戎屠美查振刚刘宏伟
Owner JINAN UNIVERSITY
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