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Biological small-diameter artificial blood vessel and preparation method thereof

A small-bore, vascular technique with applications in biomedical engineering

Active Publication Date: 2012-12-26
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is also a domestic patent (CN200510110957.2) for the preparation of artificial biological valves using PC cross-linked decellularized heart valves, but the structure of the heart valve is relatively simple, with only outer endothelial cells and inner mesenchymal cells, which is far less complex than the structure of the blood vessel wall

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  • Biological small-diameter artificial blood vessel and preparation method thereof
  • Biological small-diameter artificial blood vessel and preparation method thereof
  • Biological small-diameter artificial blood vessel and preparation method thereof

Examples

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preparation example Construction

[0059] In a specific embodiment, the preparation method of the present invention mainly includes the steps of decellularization treatment and cross-linking treatment.

[0060] In a specific embodiment, the preparation method of the present invention comprises:

[0061] (1) obtaining mammalian small-caliber blood vessel material;

[0062] (2) performing decellularization on the small-diameter blood vessel material to obtain a natural extracellular matrix that still preserves the shape of the blood vessel; and

[0063] (3) Using proanthocyanidins to cross-link the natural extracellular matrix.

[0064] Herein, small-diameter blood vessels refer to blood vessels with an inner diameter of 6 mm or less in mammals.

[0065] The present invention mainly uses small-diameter blood vessels of mammals (preferably larger mammals, such as pigs, cows, rabbits, sheep and dogs, etc.) to prepare the artificial small-diameter blood vessels of the present invention. Preferably, bovine or porc...

Embodiment 1

[0102] Example 1: Preparation of decellularized bovine carotid arteries with small caliber

[0103] 1. Decellularization of small-bore vascular raw materials

[0104] In this example, decellularized bovine carotid artery small-diameter blood vessels were used as raw materials, and the decellularized treatment included:

[0105] (1) Take fresh bovine carotid artery small-caliber blood vessels from the slaughterhouse, clean them three times with D-Hanks solution after removing fat and other sundries in a sterile environment;

[0106] (2) Soak in D-Hanks solution at 37°C for 30 minutes, perfuse 1-5 mL of preheated (about 37°C) D-Hanks solution with a concentration of 0.25% trypsin per centimeter of blood vessel length; then soak it for 37 minutes Digest at ℃ for 5 minutes;

[0107] (3) Perfuse 1-5 mL of D-Hanks solution below 4°C per centimeter of blood vessel length to stop digestion, and then wash with D-Hanks solution three times.

[0108] (4) Immerse the small-diameter blo...

Embodiment 2

[0117] Example 2: Performance testing of cross-linked decellularized small-bore blood vessels

[0118] The following nine methods were used to evaluate the properties of the cross-linked decellularized small-diameter blood vessels prepared by the method in Example 1.

[0119] 1. Paraffin-embedded routine pathological section staining (HE staining) observation

[0120] The small-diameter blood vessels prepared according to the method of Example 1 were soaked and fixed in 4% formalin for 3 days; they were soaked in a series of ethanol solutions in sequence (volume fractions of ethanol were respectively 30%, 50%, 70% , 90%, 95%, 100%), each concentration was dehydrated for 10 minutes; the sample was immersed in conventional molten paraffin liquid for embedding, after sectioning, HE staining was performed by conventional methods, and observed under a microscope for cells or Cell debris remained and photographed. In addition, the PC-crosslinked decellularized small-diameter blood...

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Abstract

The invention discloses a biological small-diameter artificial blood vessel and preparation method thereof. The method comprises a step of crosslinking the natural extracellular matrix of vessels by using procyanidins. The small-diameter artificial blood vessel of the invention has the characteristics of improved mechanical strength and stability, non-toxicity, enzymatic degradation resistance in vivo, anti-calcification, platelet adhesion resistance, low immunogenicity, and very little protein release from cross-linking agent and the small-diameter vessel. These crosslinking characteristics are beneficial for the immigration, prolongation, and long-term anti-calcification of the small-diameter vascular smooth muscle cells and vascular endothelial cells of a human body itself after the small-diameter artificial blood vessel is implanted in the human body.

Description

technical field [0001] The invention belongs to the field of biomedical engineering and relates to a cross-linking preparation method of artificial small-diameter blood vessels. technical background [0002] Cardiovascular disease and peripheral vascular disease have high morbidity, disability and mortality [1]. In 2003, the World Health Organization reported that about 16.7 million people died of cardiovascular disease in the world every year, accounting for 20% of the total death toll. 1 / 3[2]. Preliminary statistics show that about 2.6 million people die of cardiovascular disease in my country every year. Every 12 seconds, one person dies from the disease, and every 15 seconds, one person loses the ability to work due to the disease. The incidence of cardiovascular disease in my country is increasing every year, and the resulting death has accounted for about 44% of the death toll [3], among which small vessel disease is more common. The usual way to treat severe small v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L33/04A61F2/06
Inventor 常江翟万银侯永泰吴剑英
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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