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Artificial nerve scaffold and preparation method and application thereof

A technology of artificial nerves and nerve guides, applied in medical science, prostheses, surgery, etc., can solve the problems of nerve diameter mismatch between donor and recipient, damaged function of donor area, difficulty in scale-up production, etc., to prevent connective Scar tissue grows in, promotes the speed of nerve regeneration, and is conducive to the effect of growing in and rebuilding

Active Publication Date: 2017-07-04
烟台蓝创生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its main disadvantages are: the source of autologous nerve is limited, the diameter of the donor and recipient nerves does not match, and cutting the autologous nerve will also cause damage to the donor area and certain dysfunction
[0005] However, there are many defects in the nerve guide products currently on the market, such as: ① involving the use of fine molds, it is difficult to scale up production; ② the form structure and material formulation of the product need to be optimized to ensure that the nerve guide has sufficient mechanical Swelling and collapse; ③ The biocompatibility and biological activity of the product need to be improved to meet the clinical requirements for repairing long-distance peripheral nerve defects

Method used

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  • Artificial nerve scaffold and preparation method and application thereof
  • Artificial nerve scaffold and preparation method and application thereof
  • Artificial nerve scaffold and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Dosing

[0038] Dissolve collagen and polylactic acid (PLA) in hexafluoroisopropanol (HFIP) at a mass ratio of 1:9, with a material-to-liquid ratio of 1:10, and stir until completely dissolved to obtain a uniform spinning solution for the outer layer of the nerve guide (O);

[0039] The collagen was dissolved in hexafluoroisopropanol (HFIP) with a material-to-liquid ratio of 1:10, and stirred until it was completely dissolved to obtain a uniform spinning solution (I) for the inner layer of the nerve guide.

[0040] Dissolve collagen in pure water so that the final concentration reaches 1 mg / ml to obtain the "natural polymer binder", and store it at 4°C after filtration and sterilization.

[0041] 2. Preparation of two-component nanofiber membrane

[0042] Nanofiber membranes with a certain orientation are obtained by electrospinning with a conjugate electrostatic spinning device, that is, the nozzles of the two spinning solutions are arranged on both sides above th...

Embodiment 2

[0051] 1. Dosing

[0052] Dissolve gelatin and polylactide caprolactone copolymer in trifluoroethanol according to the mass ratio of 2:8, the ratio of solid to liquid is 1:20, and stir until completely dissolved to obtain a uniform outer layer spinning solution of nerve guide (O) ;

[0053] Gelatin and polylactide caprolactone were dissolved in trifluoroethanol at a mass ratio of 50:1, and the material-to-liquid ratio was 1:20, and stirred until completely dissolved to obtain a uniform inner layer spinning solution (I) of the nerve conduit.

[0054] Dissolve gelatin in pure water so that the final concentration reaches 3mg / ml to obtain the "natural polymer binder", and store it at 4°C after filtration and sterilization.

[0055] 2. Preparation of two-component nanofiber membrane

[0056] Nanofiber membranes with a certain orientation are obtained by electrospinning with a conjugate electrostatic spinning device, that is, the nozzles of the two spinning solutions are arranged...

Embodiment 3

[0065] 1. Dosing

[0066]Dissolve chitosan and polylactide caprolactone copolymer in acetone / acetic acid mixed solvent (volume ratio 20:1) according to the ratio of mass ratio 4:6, and the ratio of solid to liquid is 1:8, and stir until completely dissolved to obtain a uniform The outer layer spinning solution (O) of the nerve guide;

[0067] Dissolve collagen and polyglycolic acid (PGA) in hexafluoroisopropanol (HFIP) at a mass ratio of 10:1, with a material-to-liquid ratio of 1:8, and stir until completely dissolved to obtain a uniform nerve guide inner layer spinning Liquid (I).

[0068] The nerve growth factor was dissolved in pure water to a final concentration of 50 μg / ml, and stirred until completely dissolved to obtain a uniform core layer solution (Y).

[0069] Dissolve sodium hyaluronate in pure water so that the final concentration reaches 10mg / ml to obtain the "natural polymer binder", and store it at 4°C after filtration and sterilization.

[0070] 2. Preparati...

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Abstract

The invention discloses an artificial nerve scaffold capable of inducing nerve regeneration active. The artificial nerve scaffold includes a dual-nerve catheter, oriented nano fiber beams and a natural polymer adhesive; the dual-nerve catheter is directly prepared outside the oriented nano fiber beams; and the natural polymer adhesive is poured into clearances of the oriented nano fiber beams to acquire the artificial nerve scaffold. the oriented nano fiber beams are rolled by a dual-component nano fiber film; the nano fiber beams are sparsely arranged, thereby facilitating growth of nerve cells; the outer layer of the nerve catheter is formed by a compact nano fiber film pipe having excellent hydrophobicity and can prevent growth of connective tissues; the inner layer of the nerve catheter is formed by a dual-component nano fiber film pipe, and is conductive to reconstruction of capillary vessels; and the natural polymer adhesive is an aqueous solution prepared by a natural polymer material, and stem cells can be added into the natural polymer adhesive. The invention discloses a preparation method of the artificial nerve scaffold, and application of the artificial nerve scaffold to peripheral nerve injury repairing.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and relates to an artificial nerve repairing material filled with active orientation nanofibers, a preparation method thereof, and an application in repairing peripheral nerve injuries. Background technique [0002] Peripheral nerve injury is a common clinical disease with poor prognosis and high disability rate, which brings huge economic loss and mental burden to patients, families and society. Therefore, how to promote nerve regeneration and restore the function of target organs has always been a hot spot of concern in the whole society. For peripheral nerve defects, autologous nerve transplantation is still the "gold standard" for repair. Its main disadvantages are: the source of autologous nerve is limited, the diameter of the donor and recipient nerves does not match, and cutting the autologous nerve will also cause damage to the donor site and certain dysfunction. Therefore, looking for...

Claims

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Application Information

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IPC IPC(8): A61F2/02A61B17/11A61L27/18A61L27/20A61L27/22A61L27/24A61L27/26A61L27/38A61L27/54A61L27/58
CPCA61B17/1128A61F2/02A61L27/18A61L27/20A61L27/222A61L27/227A61L27/24A61L27/26A61L27/3878A61L27/54A61L27/58A61L2300/216A61L2300/252A61L2300/414A61L2300/43A61L2430/32
Inventor 李东升曲桂武杨小平张淑敏宫世周高久香刘慧萍
Owner 烟台蓝创生物技术有限公司
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