74results about How to "Achieve in situ regeneration" patented technology

The invention discloses a gene recombination engineering bacterium and its application in catalyzing the synthesis of D-pantolactone, which belongs to the field of biocatalysis research. The genetically recombined engineering bacterium uses E. coli as a host bacterium, and the host bacterium contains a recombinant expression plasmid comprising a conjugated polyketide reductase gene and a glucose dehydrogenase gene, and the conjugated polyketide reductase gene The nucleotide sequence is shown in SEQ ID NO.1. The engineering bacterium is induced to express polyketide reductase and glucose dehydrogenase at the same time, and it is applied to the asymmetric catalytic synthesis of D-pantolactone. Through the synergistic effect of the above two enzymes, the in situ regeneration of the coenzyme NADPH can be realized The purpose of high-efficiency production from ketopantoolactone to D-pantoolactone is achieved. The method simplifies the production process, avoids the addition of extra coenzymes and reduces the production cost.

The invention discloses a method and device for treating oily wastewater with activated carbon fiber and its regeneration method and device. Specifically, the method disclosed in the invention uses an electrochemical reaction device in which activated carbon fiber felt is used as the anode and mesh-shaped titanium metal is used as the cathode. The combination of adsorption-electrolysis-desorption of oil in oily wastewater and regeneration of activated carbon fibers was studied. This electrochemical reaction device is composed of a reactor, an electric mixer and an externally connected regulated DC power supply. The reactor is equipped with multiple sets of detachable anodes and cathodes. The anode is composed of activated carbon fiber felt as the main body and titanium-based metal oxide as the support. The net-like pure titanium metal is the cathode electrode, and the anode and cathode groups are alternately connected by wires. The power supply and the number of electrode groups can be disassembled to increase or decrease, and the number of electrode groups can be adjusted according to the processing requirements. Compared with the existing technology, the method of the present invention realizes the integration of activated carbon fiber electrochemical treatment of oily wastewater and its in-situ regeneration technology, prolongs the regeneration cycle, reduces the process flow and secondary pollution, is easy to operate and control, and is convenient industrial applications.

The invention belongs to the biology and biological technical field, and discloses a plant in-situ regeneration and agrobacterium transformation method. Agrobacterium is coated on a wound of a plant root stock, a callus which is generated by inducing a plant in-vitro tissue on a culture medium is used as a scion, and the scion is grafted onto the root stock through a cleft graft method. Root stock wound cells can realize in-situ organ regeneration under the induction of an exogenous callus, so that a transgenic regeneration bud can be obtained. Compared with the traditional plant genetic transformation method combining the tissue culture, a one-by-one genotype fumbling tissue culture condition can be avoided, a novel technological platform can be provided to the genetic transformation of plants, meanwhile, a novel plant regeneration system is established, and a research system is provided to the study of plant development biology.

A method of a microwave intermittently liquid activated carbon desulfurization and denitrification. It is used to adsorb or microwave radiation treatment of boiler flue gas with two or more adsorption beds equipped with activated carbon particles., The adsorption bed enters the working state, and the sulfur nitrogen oxide in the flue gas in the activated carbon has the activated carbon inhalation of the active carbon suction to be saturated after the working state.The smoke adsorption of the bed, the recycled recycled state adsorption bed adopts microwave liquid liquid carbon particles, and sulfur nitrogen oxides that are adsorbed on the surface of the activated carbon surface are quickly restored to monocular sulfur and nitrogen by charcoal at high temperatures.Alternate cycle.In order to reduce the reduction of degradation temperature and reduce carbon loss, metal oxides were used as a reaction catalyst.The present invention can greatly reduce the energy consumption of microwave radiation, reduce the loss of carbon, and achieve the regeneration of activated carbon in situ. It has the characteristics of good removal effect, short activated carbon regeneration time, low removal cost, high recovery rate, and easy industrialization implementation.