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Double-silk fibroin nerve graft

A silk fibroin and nerve transplantation technology, applied in the field of biomedical materials, can solve the problems of lack of flexibility and strength, poor mechanical properties of scaffolds, etc., and achieve the effect of improving mechanical properties, good mechanical properties, and good tissue compatibility.

Inactive Publication Date: 2018-06-05
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, silk fibroin tissue engineering scaffolds prepared by a single traditional method (such as freeze-drying, electrospinning, rotary evaporation, etc.) have poor mechanical properties, especially in terms of flexibility and strength, and cannot meet clinical requirements.

Method used

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  • Double-silk fibroin nerve graft
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of double silk fibroin nerve graft described in embodiment 1 of the present invention

[0033] (1) The preparation of mulberry silk fibroin fiber: get mulberry silk, in 0.2% Na 2 CO 3 Boil in the solution to remove the outer sericin, repeat 3 times for 30 minutes each time, thoroughly wash with three-distilled water, and then place it in an ultra-clean bench to dry to obtain silk fibroin fiber.

[0034] (2) Preparation of tussah silk fibroin protein: get 25g tussah silk, put it in 1000ml0.2% Na 2 CO 3 Boil in the solution for 30 minutes each time, repeat 3 times, thoroughly wash with triple-distilled water, and then place it on a super-clean table to dry to obtain tussah silk fibroin.

[0035] Preparation of regenerated tussah silk fibroin protein: get 6 g of tussah silk fibroin protein prepared above and dissolve in 25 mL lithium thiocyanate solution (9mol / L) to obtain a brown solution. The dissolution temperature is 40° C., and the solution is packe...

Embodiment 2

[0039] The preparation of embodiment two control catheters

[0040] Referring to the method of Example 1, a catheter with silk fibroin skeleton and shell (control 1) was prepared.

[0041] The 3% regenerated tussah silk fibroin protein solution is concentrated to 5-10%, poured into a mold, and freeze-dried at -70°C to form a conduit. The obtained catheter was soaked in 60% ethanol for 12 hours to make it denatured, then soaked and washed with deionized water, and dried naturally to obtain a catheter with only tussah silk fibroin shell (control 2).

Embodiment 3

[0042] Example 3 Investigation of Cell Adhesion and Growth Performance

[0043] (1) Preparation of mulberry silk fibroin film: the mulberry silk fiber obtained in Example 1 (1) is dissolved in a ternary solvent system (anhydrous CaCl 2 : Anhydrous C 2 h 5 OH: triple distilled water = 1:2:8) to obtain a yellow transparent solution with a dissolution temperature of 73±2°C. The solution was placed in a cellulose tube (molecular weight cut-off: 12-14KD), and dialyzed with triple distilled water for 3 days. The dialyzed silk fibroin solution was poured into a petri dish, and placed in an ultra-clean bench to air dry. After being dried, it is peeled off to form a silk fibroin film.

[0044] (2) Preparation of tussah silk fibroin protein film: Pour the tussah silk fibroin protein solution dialyzed in Example 1 (2) into a petri dish, and put it in an ultra-clean table to blow air to dry. After drying, it is peeled off to form a tussah silk film.

[0045] (3) Cell culture: steril...

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Abstract

The invention discloses a double-silk fibroin nerve graft. The double-silk fibroin nerve graft is characterized by comprising a skeleton and a tussah silk fibroin shell, wherein the skeleton is produced by mulberry silk fibers through weaving. By combining the property characteristics of mulberry silks and tussah silks, the mechanical properties of a silk fibroin nerve graft are improved. The prepared double-silk fibroin nerve graft has high mechanical strength and good flexibility and can resist the stretching of a suture line, and when the formation of a conduit is 50%, the compressive strength can reach 1.8N; and the double-silk fibroin nerve graft has very important clinical significances to the rapid acceleration of nerve regeneration and the long-distance restoration of defected nerves.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a double silk fibroin nerve graft for bridging defective nerves. Background technique [0002] Nerve injury is a common clinical disease. The loss of body function caused by it brings great harm and inconvenience to patients, and also brings heavy economic and mental burdens to families and even the whole society. The peripheral nervous system has a certain ability to regenerate. For short-distance nerve defects, the damaged nerve can regenerate itself and re-establish corresponding functions. However, the repair of long-distance defects requires the help of nerve grafts. Although autologous transplantation is still the main method for repairing nerve defects, its efficacy is limited and there are many shortcomings, such as lack of sources, denervation dysfunction at the donor site, and difficulty in matching the size and tissue structure of the donor. Therefore, people bega...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/38A61L27/50A61L27/54A61L27/56
CPCA61L27/227A61L27/383A61L27/50A61L27/54A61L27/56A61L2300/414A61L2430/32C08L89/00
Inventor 杨宇民王亚玲邵健李贵才顾晓松凌珏
Owner NANTONG UNIVERSITY
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