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Nerve graft preparing method and product of nerve graft

A nerve transplantation and nerve technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of lack of biological components for nerve regeneration and growth, lack of supporting tissue skeleton, unsatisfactory effect, etc. The effect of improving structural integrity

Active Publication Date: 2016-09-07
ZHEJIANG YUANTAI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these catheters lack biological components that promote nerve regeneration and growth, and there is no tissue skeleton to support nerve growth, so the effect is not ideal

Method used

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  • Nerve graft preparing method and product of nerve graft
  • Nerve graft preparing method and product of nerve graft

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A preparation method of a nerve graft, the preparation process is as follows figure 1 As shown, pig nerve tissue is used as raw material, including freeze-thaw and chemical washing process, including the following steps:

[0053] 1) Extraction of porcine nerve tissue

[0054] Extract sciatic nerve, tibial nerve and common peroneal nerve tissues from pigs aged 6-12 months, and obtain them 3-6 hours after slaughter. The length of the nerve tissue is 10-30cm, and the diameter is 2-6mm;

[0055] 2), remove the fat tissue and connective tissue connected with the nerve tissue

[0056] Rinse 5 times with sodium phosphate buffer, place in sodium phosphate buffer, and store in -80°C refrigerator for later use;

[0057] 3), liquid nitrogen freeze-thaw

[0058] The nerve tissue in step 2) was rinsed with distilled water at room temperature, placed in liquid nitrogen for 2 minutes, and then melted in a water bath at 37°C, and repeated 5 times.

[0059] 4), chemical washing

[00...

Embodiment 2

[0072] Compared with Example 1, the difference lies in the times of freezing and thawing of liquid nitrogen and the time of shaking and rinsing during chemical washing:

[0073] Liquid nitrogen freeze-thaw repeated 3 times;

[0074] For chemical washing, rinse the nerve tissue after freezing and thawing with liquid nitrogen in step 3) with distilled water at room temperature, place it in solution A, shake and wash for 5 hours; then place it in solution B, shake and wash for 10 minutes;

[0075] Place in solution C, shake and rinse for 12 hours; then place in solution B, shake and wash for 5 minutes, repeat 3 times; place in solution A, shake and wash for 5 hours; place in solution B, shake and wash for 10 minutes;

[0076] Place in solution C, shake and rinse for 10 hours; finally place in solution B, shake and wash for 5 minutes, repeat 3 times.

Embodiment 3

[0078] Compared with Example 1, the difference lies in the times of freezing and thawing of liquid nitrogen and the time of shaking and rinsing during chemical washing:

[0079] Liquid nitrogen freeze-thaw repeated 3 times;

[0080] For chemical washing, rinse the nerve tissue after freezing and thawing with liquid nitrogen in step 3) with distilled water at room temperature, place it in solution A, shake and wash for 20 hours; then place it in solution B, shake and wash for 30 minutes;

[0081] Place in solution C, shake and rinse for 36 hours; then place in solution B, shake and wash for 10 minutes, repeat 3 times; place in solution A, shake and wash for 20 hours; place in solution B, shake and wash for 30 minutes; In solution C, shake and rinse for 24 hours; finally place in solution B, shake and wash for 10 minutes, repeat 3 times.

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Abstract

The invention discloses a nerve graft preparing method and a product of a nerve graft. The preparing method comprises the following steps of extracting pig nervous tissue, removing adipose tissue and connective tissue which are connected with the nervous tissue, freeze thawing through liquid nitrogen, chemically washing and sterilizing. According to the nerve graft preparing method, two methods of freeze thawing and chemical washing are combined. Thus, the structural integrity of a nerve tissue skeleton is improved, cellular components in the pig nervous tissue are completely removed, and the immunity is reduced. The nerve graft product prepared from the nerve graft can achieve goals of quick growth of nerves and functional recovery and provides a feasible program for clinical treatment.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a preparation method of a nerve graft, and also relates to a nerve graft product prepared by the method. Background technique [0002] Peripheral nerve injuries in humans are very common, mainly occurring in traumatic accidents or surgical operations. Nerve rupture often leads to loss of human function and even disability. Therefore, nerve rupture requires timely surgical repair. The basic method of nerve repair is to suture the two broken ends, but in general, nerve damage results in the loss of a section of nerve, forming a gap and requiring nerve transplantation. [0003] After nerve injury, the distal nerve degeneration, necrosis, nerve tissue is absorbed, but the skeletal pipeline supporting the nerve still exists. Proximal nerves have the ability to regenerate. In the presence of intact neuroskeletal conduits, proximal nerve endings grow and can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
CPCA61L27/3675A61L27/3687A61L27/3691A61L2430/32A61L2430/40
Inventor 陈焕新胡少青徐永军
Owner ZHEJIANG YUANTAI BIOTECH CO LTD
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