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Nerve graft conduit based on chitosan and preparation method thereof

A chitosan and graft technology, which is applied in the field of biomedical materials, can solve problems such as adverse reactions, defective nerve repair functions, and can not perfectly match nerve defects in different parts, and achieves good biocompatibility and good use effect.

Pending Publication Date: 2021-10-26
JIANGSU YITONG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above three patents all disclose the preparation technology of nerve repair grafts. Nerve repair grafts are prepared through specific raw materials and processes, which have the function of repairing defective nerves. However, the degradation time of the prepared grafts is uncontrollable and cannot be perfectly matched. Nerve defects of different parts and different lengths and thicknesses lead to the nerve repair grafts prepared by the above-mentioned prior art often have the situation that the nerves have not grown completely, and the grafts have decomposed, and the repair effect cannot be effectively guaranteed; The degradation is slow, and the graft exists in the human body for a long time after the nerve repair is completed, resulting in adverse reactions

Method used

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  • Nerve graft conduit based on chitosan and preparation method thereof
  • Nerve graft conduit based on chitosan and preparation method thereof
  • Nerve graft conduit based on chitosan and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0032] The preparation method of chitosan-based nerve graft conduit comprises the following steps:

[0033] Step 1. Design the molecular weight range of chitosan and the porosity of the catheter according to the required degradation time of the catheter.

[0034] Step 2, refining chitosan to obtain refined chitosan. The specific method for refining chitosan is: put chitosan raw materials into NaOH or KOH solution with a mass concentration of 10-80%, react at a temperature of 30-150°C for 1-24 hours, wash and dry to obtain refined chitosan Chitosan.

[0035] Step 3, as shown in Table 1, the chitosan molecular weight intercepted by molecular screening membranes with different apertures is different; molecular screening membrane is used to carry out molecular weight screening to purified chitosan, and a series of chitosans with different molecular weights are separated.

[0036]The specific method of using molecular screening membrane to separate chitosan is: dissolve the purif...

Embodiment 1

[0043] Example 1 Making a nerve guide with a degradation time of 40 days

[0044] Step 1, the medium-term use catheter with a degradation time of 40 days, the chitosan molecular weight ranges from 100,000 to 150,000, and the tube porosity is 70%.

[0045] Step 2, putting the chitosan raw material into NaOH solution with a mass concentration of 30%, reacting at 100° C. for 8 hours, washing with water and drying to obtain refined chitosan.

[0046] Step 3, dissolving the refined chitosan, and selecting two ultrafiltration membranes with corresponding pore sizes according to the upper limit and lower limit of the molecular weight distribution of chitosan designed in step 1, the pore sizes are 0.01 μm and 0.05 μm respectively. The chitosan solution passes through an ultrafiltration membrane with a pore size of 0.05 μm and an ultrafiltration membrane with a pore size of 0.01 μm in turn, intercepting the solution between the two layers of ultrafiltration membranes, and drying to obt...

Embodiment 2

[0049] Example 2 Making a nerve guide with a degradation time of 20 days

[0050] Step 1. For short-term use catheters with a degradation time of 20 days, the chitosan molecular weight ranges from 10,000 to 20,000, and the catheter porosity is 80%.

[0051] Step 2, putting the chitosan raw material into NaOH solution with a mass concentration of 30%, reacting at 100° C. for 8 hours, washing with water and drying to obtain refined chitosan.

[0052] Step 3, dissolving the purified chitosan, according to the upper limit and lower limit of the molecular weight distribution of chitosan designed in step 1, select two ultrafiltration membranes with corresponding apertures, the apertures are respectively 0.005 μm and 0.006 μm. The chitosan solution passes through an ultrafiltration membrane with a pore size of 0.006 μm and an ultrafiltration membrane with a pore size of 0.005 μm in turn, intercepting the solution between the two layers of ultrafiltration membranes, and drying to obta...

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Abstract

The invention relates to a nerve graft conduit based on chitosan and a preparation method thereof. The nerve graft conduit based on chitosan is the conduit prepared by taking chitosan as a main raw material, the degradation time in a human body is accurately controlled to be 1 week to 6 months, the porosity of the conduit is 65-85%, the molecular weight distribution of the chitosan is 10K-500k, according to the porosity and the molecular weight of chitosan, the conduit can be divided into a short-term-use conduit, a middle-term-use conduit and a long-term-use conduit , the nerve conduit has good biocompatibility with the human body, the nerve conduit with corresponding repair time can be made according to nerves at different parts, different defect lengths and the thickness of defective nerves, the nerve graft conduit meets the nerve repairing growth rule, the use effect is good, and the nerve graft conduit can be widely applied clinically.

Description

【Technical field】 [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a chitosan-based nerve graft catheter and a preparation method thereof. 【Background technique】 [0002] Clinically, peripheral nerve defects caused by trauma or surgery are more common, but in the case of long peripheral nerve loss, the nerve repair cannot be completed by directly suturing the nerve stumps on both sides. Nerve allografts, xenogeneic nerve grafts, or artificially synthesized nerve grafts can be used to repair nerve conduits. However, the source of autologous nerve transplantation and decellularized allogeneic nerve transplantation is limited, and it will cause damage to the donor site. The method of xenogeneic nerve transplantation has the risk of spreading diseases and viruses. Artificially synthesized nerve transplantation is currently an ideal method. A form of neural repair. Therefore, it is an urgent clinical need to prepare nerve...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/56A61L27/50
CPCA61L27/20A61L27/56A61L27/50A61L2430/32C08L5/08
Inventor 张添赵敏健姜育辉唐璐冯珊珊
Owner JIANGSU YITONG BIOTECHNOLOGY CO LTD
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