Melatonin nerve conduit composition and nerve conduit, as well as preparation method and application thereof

Abstract

The invention provides a melatonin nerve conduit composition and a nerve conduit, as well as a preparation method and application thereof. The composition comprises a biodegradable material, melatoninand a biocompatible adhesive material; various conduits applied to biomedicine are molded by utilizing 3D printing or co-melting of an electrostatic spinning biodegradable material and melatonin or an organic solvent; and after the conduits are molded, a biological adhesive substance layer is printed or coated to the inner wall according to requirements, and cells are induced to differentiate andgrow in conduits. The invention further provides a conduit bracket essential to biology, which is prepared from an ideal biomedicinal functional material, and has the advantages of simple preparation, low cost, easily controlled quality, wide application and the like. Sciatic nerve defect repairing experiments for rats indicate that the melatonin nerve conduit can be used for promoting nerve regeneration, improving maturation of regenerated nerves, increasing the number of effective nerve fibers and promoting functions of sciatic nerves to be restored to normal, has the effect superior to autologous nerve grafting restoration, and has a good application prospect.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a melatonin nerve guide, a preparation method and an application thereof. Background technique [0002] Various biomedical catheters currently include degradable and non-degradable catheters. For example, polyethylene tubes have been used as nerve catheters to study nerve regeneration. Due to the non-degradable catheters, after the completion of nerve regeneration, if the catheters are not treated, it may cause Tissue fibrosis causes toxic and side effects such as inflammation, and the clinical application effect is not very satisfactory. At present, various biodegradable materials have been developed to prepare various biomedical catheters, such as collagen, polylactic acid, etc., but because these materials are used alone, there are some problems such as strength, nerve regeneration speed, and toxic side effects. Recently, it has been developed to further research on these ...

AI Technical Summary

Problems solved by technology

[0004] Although some research progress has been made on nerve guides, such as the Chinese invention patent CN100479785C which discloses a method for preparing nerve guides, there is still a certain distance from autologous nerve repair.

In addition, there are many patents disclosing different preparation methods (such as Chinese invention patent CN101439205A, open date 2009.5.27; CN101507842A, open date 2009.8.19, CN106668938A, open date 2017.05.17; CN106924820A, open date 2017.07.07), But these catheters either have issues with strength, toxicity, quality control or cost

Chinese patent 2010800025691 discloses a method and equipment for preparing a three-dimensional porous tubular scaffold. It specifically discloses that the 3D tubular scaffold can be made of non-biodegradable polymers, biodegradable polymers or combinations thereof. Biodegradable polymers include, for example , polylactic acid (PLA), polyglycolic acid (PGA), etc., 3D tubular scaffolds can be added with hormones, examples of hormones include melatonin, etc., 3D tubular scaffolds can also be used as peripheral nerve conduits to guide damaged nerve regeneration; Although the patent discloses the use of melatonin and polylactic acid to prepare nerve conduits, it does not disclose the role of melatonin in promoting nerve repair

None of the nerve guides disclosed in the prior art are ideal biomedical guides

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